Contamination Control during Tissue Culture of Peony

doi:10.7525/j.issn.1673-5102.2012.01.014

Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (1): 84-90.doi: 10.7525/j.issn.1673-5102.2012.01.014

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Contamination Control during Tissue Culture of Peony

WANG Ji-Feng;LI Qing;BAO Xin

School of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Flowers,Beijing　100083

Received:1900-01-01 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20
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Abstract

Abstract: To solve the problem of explant contamination during peony tissue culture, the explants of different seasons were studied. The results showed that soaking explants in 0.1%-0.2% HgCl2 for 8 min, etiolation treatment, low temperature and electronic vibration are effective for sterilization; Antibiotics added into the media can inhibit contamination caused by endophytes.

Key words: peony, explant, tissue culture, contamination, antibiotics

CLC Number:

S682.1+2

WANG Ji-Feng;LI Qing;BAO Xin. Contamination Control during Tissue Culture of Peony[J]. Bulletin of Botanical Research, 2012, 32(1): 84-90.

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Contamination Control during Tissue Culture of Peony

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