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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (2): 243-247.doi: 10.7525/j.issn.1673-5102.2013.02.021

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ISSR Identification of Clone Plants of Solanum tuberosum Hybrids F1

GAN Lin;YU Xiao-Xia;JU Tian-Hua;YU Zhuo*;MENG Mei-Lian;ZHANG Zi-Qiang;LI Chang-Qing   

  1. 1.College of Agronomy,Inner Mongolia Agricultural University,Hohhot　010019；2.Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture,China Agricultural University,Beijing　100193；3.Chifeng Tianze BioTechnology CO,LTD,Chifeng　025457
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
  • Contact: YU Zhuo
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Abstract: In order to provide reliable materials for potato varieties breeding, the study was to identify the authenticity of clones of hybrids F1 of two potato combinations, ‘J07-4’בLongshu No.6’ and ‘J07-6’בLongshu No.6’, using ISSR molecular markers. The results were that five primers were selected from 152 ISSR primers, primers AF18550, AW75511 and AW20617 were suitable to identify 5 clones of ‘J07-4’בLongshu No.6’ hybrids F1, and AW20607 and AF18549 were suitable to identify 7 clones of ‘J07-6’בLongshu No.6’ hybrids F1. A total of 12 clones were identified as true hybrids based on the paternal-specific bands in progeny’s bands. ISSR fingerprints of different clones of hybrids F1 were established. It showed that it was feasible to use ISSR molecular markers to identify clones of potato hybrids F1.

Key words: Potato, hybrids F1, clones, ISSR identification

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