Abstract: A new typical and high efficient rooting and transplanting method of Populus davidiana×P.bolleana was set up from hardening off seedlings, moisturizing and pollution prevention in the study. Firstly, put the tissue culture P.davidiana×P.bolleana seedlings in large test tubes with liquid medium for rooting for 12-15 d. Secondly, refine hydroponic seedlings in the aseptic water for 2 d. Then, transplant the seedlings into soil and cover with plastic cups to moisturizing, which will be removed after 15 d. At last, the 17 d old seedlings with soil were transplanted into the field. Using the above method, the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic, simple, small-scaled and high efficient but also suitable for many other woody plants, so it is easy to generalize.

Key words: Populus davidiana×, P.bolleana, tissue culture, rooting, transplanting, survival rate