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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (3): 380-385.doi: 10.7525/j.issn.1673-5102.2014.03.015

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Rooting and Transplanting Method of Populus davidiana×P.bolleana Tissue Culture Seedlings

WANG Na;DOU Kai;WANG Zhi-Ying;ZHANG Rong-Shu;WANG Yu-Cheng;LIU Zhi-Hua*   

  1. 1.State Key Laboratory of Tree Genetics Breeding,Northeast Forestry University,Harbin 150040;2.School of Forestry,Northeast Forestry University,Harbin 150040;3.College of Landscape,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: LIU Zhi-Hua
  • Supported by:

Abstract: A new typical and high efficient rooting and transplanting method of Populus davidiana×P.bolleana was set up from hardening off seedlings, moisturizing and pollution prevention in the study. Firstly, put the tissue culture P.davidiana×P.bolleana seedlings in large test tubes with liquid medium for rooting for 12-15 d. Secondly, refine hydroponic seedlings in the aseptic water for 2 d. Then, transplant the seedlings into soil and cover with plastic cups to moisturizing, which will be removed after 15 d. At last, the 17 d old seedlings with soil were transplanted into the field. Using the above method, the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic, simple, small-scaled and high efficient but also suitable for many other woody plants, so it is easy to generalize.

Key words: Populus davidiana×, P.bolleana, tissue culture, rooting, transplanting, survival rate

CLC Number: