Abstract: A dirigent-like protein (DIR) full length cDNA was cloned from Tamarix androssowii cDNA library. The Tadir gene is 724 bp in length without its polyA sequence, including 26 bp of 5′untranslated region and 143 bp of 3′untranslated region. It has an open reading frame (ORF) of 555 bp, encoding 184 amino acid residues. The protein molecular weight is 19.69 kD and its isoelectric point is 6.96. Hydrophobicity analysis showed that there was a hydrophilic region between 1 to 41 residues. This gene was accepted by Genbank, the accession numbers are DQ462418 (gene) and ABE73781 (protein). Under the stress of 0.4 mol·L^-1 NaCl and NaHCO₃, expression of Tadir gene has been changed by real-time PCR. Therefore, this gene may be associated with salt resistance of T.androssowii.

Key words: Tadir gene, gene cloning, sequence analysis, Tamarix androssowii, real-time PCR