Effects of Nitrogen Addition on Root Growth, Biomass Allocation and Non-structural Carbohydrate Content of Cinnamomum bodinier Seedlings in Rare Earth Tailings

doi:10.7525/j.issn.1673-5102.2024.01.011

Abstract

Abstract:

In order to explore the effects of nitrogen addition on the growth and non-structural carbohydrate（NSC） content of Cinnamomum bodinieri seedlings in rare earth mine tailings， one-year-old C.bodinieri cuttings were used as materials， and calcium ammonium nitrate was used as nitrogen fertilizer（containing N 15%）， and three nitrogen levels（CK（0）， N1（1.8 g per plant）， N2（3.6 g per plant）） were set up to analyze the differences in root growth， biomass distribution and NSC of C.bodinieri seedlings under three nitrogen addition levels， and to explore the response of C.bodinieri in rare earth tailings to nitrogen addition. The results showed that： Nitrogen addition increased the biomass accumulation of different tissues of C.bodinieri seedlings in rare earth tailings， and the leaf biomass and canopy biomass at N1 level were significantly increased by 44.75% and 57.43%（P<0.05）， respectively， compared with N2. Compared with CK and N1 treatment， the leaf mass ratio of N2 level was significantly increased by 123.53% and 15.85%（P<0.05）， respectively. Nitrogen addition significantly promoted the root length and root surface area of both coarse roots（diameter>2mm） and fine roots（diameter≤2mm）（P<0.05）， and the promotion effect of N1 treatment was the most significant（P<0.05）. The specific root length and specific surface area under N2 treatment were higher than those of CK and N1， and significantly different from CK（P<0.05）. For NSC， N1 treatment increased the soluble sugar mass fraction in leaves and stems， while the NSC mass fraction of fine roots under N2 treatment was significantly lower than that of CK and N1 by 46.49% and 28.61%， respectively. In summary， during vegetation restoration in rare earth mine tailings， 1.8 g per plant calcium ammonium nitrate fertilizer might be suitable for fertilization management of C.bodinieri seedlings.

Key words: rare earth mine tailings, nitrogen addition, Cinnamomum bodinieri, root growth, biomass allocation, non-structural carbohydrate

CLC Number:

Q948

Zhiqi CHEN, Haina ZHANG, Jiali LIU, Xianghui LU, Baocheng YANG. Effects of Nitrogen Addition on Root Growth, Biomass Allocation and Non-structural Carbohydrate Content of Cinnamomum bodinier Seedlings in Rare Earth Tailings[J]. Bulletin of Botanical Research, 2024, 44(1): 86-95.

Figures/Tables 5

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References 45

1	郭钟群，赵奎，金解放，等.离子型稀土矿环境风险评估及污染治理研究进展［J］.稀土，2019，40（3）：115-126.
	GUO Z Q， ZHAO K， JIN J F，et al.Reviews on environmental assessment and pollution prevention of ion adsorption type rare earth ores［J］.Chinese Rare Earths，2019，40（3）：115-126.
2	DAI W J， ZHANG P， YANG F，et al.Effects of composite materials and revegetation on soil nutrients，chemical and microbial properties in rare earth tailings［J］.Science of the Total Environment，2022，850：157854.
3	张海娜，鲁向晖，金志农，等.高温条件下稀土尾砂干旱对4种植物生理特性的影响［J］.生态学报，2019，39（7）：2426-2434.
	ZHANG H N， LU X H， JIN Z N，et al.Effects of drought on physiological characteristics of seedlings of four species grown on rare earth mill tailings at high temperatures［J］.Acta Ecologica Sinica，2019，39（7）：2426-2434.
4	BARŁÓG P， GRZEBISZ W， ŁUKOWIAK R.Fertilizers and fertilization strategies mitigating soil factors constraining efficiency of nitrogen in plant production［J］.Plants，2022，11（14）：1855-1855.
5	YAN G Y， XING Y J， HAN S J，et al.Long-time precipitation reduction and nitrogen deposition increase alter soil nitrogen dynamic by influencing soil bacterial communities and functional groups［J］.Pedosphere，2020，30（3）：363-377.
6	LIN J X， WANG Y N， SUN S N，et al.Effects of arbuscular mycorrhizal fungi on the growth，photosynthesis and photosynthetic pigments of Leymus chinensis seedlings under salt-alkali stress and nitrogen deposition［J］.Science of the Total Environment，2017，576：234-241.
7	高钿惠，王卫锋，尚佳州，等.中金杨幼苗叶功能性状与碳氮分配对氮添加的响应［J］.生态学杂志，2022，41（5）：841-848.
	GAO D H， WANG W F， SHANG J Z，et al.Responses of leaf functional traits and carbon and nitrogen allocation in hybrid poplar （Populus×euramer-icana‘Zhongjin2’） seedlings under two-year nitrogen addition［J］.Chinese Journal of Ecology，2022，41（5）：841-848.
8	高晋丽，宋艳宇，宋长春，等.不同氮素添加量对大兴安岭冻土区泥炭地植物细根形态的影响模拟研究［J］.生态学报，2021，41（20）：8171-8177.
	GAO J L， SONG Y Y， SONG C C，et al.Effects of different concentrations of nitrogen additions on fine root morphological characteristics in permafrost peatland in the Daxing’an Mountains［J］.Acta Ecologica Sinica，2021，41（20）：8171-8177.
9	董姣姣，龚吉蕊，翟占伟，等.内蒙古温带草原大针茅叶片光合生理特性对氮添加的响应［J/OL］.生态学报（2023-03-23）［2023-07-19］..
	DONG J J， GONG J R， ZHAI Z W，et al.Response of the photosynthetic physiological characteristics to nitrogen addition of Stipa grandis leaves in a temperate grassland of the Inner Mongolia［J/OL］.Acta Ecologica Sinica（2023-03-23）［2023-07-19］..
10	裴昊斐，高卫东，方娇阳，等.模拟氮沉降对一年生香椿幼苗生长和光合特性的影响［J］.中国生态农业学报（中英文），2019，27（10）：1546-1552.
	PEI H F， GAO W D， FANG J Y，et al.Effects of simulated nitrogen deposition on growth and photosynthetic characteristics of one-year-old Toona sinensis seedlings［J］.Chinese Journal of Eco-Agriculture，2019，27（10）：1546-1552.
11	WIŚNIEWSKA-KADŻAJAN B， JANKOWSKI K.Effect of slurry used with soil conditioners and fertilizers on structural，non-structural carbohydrate and lignin content［J］.Agronomy Journal，2021，113（3）：2812-2820.
12	LATT C R， NAIR P K R， KANG B T.Reserve carbohydrate levels in the boles and structural roots of five multipurpose tree species in a seasonally dry tropical climate［J］.Forest Ecology and Management，2001，146（1/3）：145-158.
13	苏原，周家如，王亭帅，等.氮添加降低盐渍化草地赖草非结构性碳水化合物含量［J/OL］.草地学报（2023-07-05）［2023-08-15］..
	SU Y， ZHOU J R， WANG T S，et al.Nitrogen addition decreased the non-structural carbohydrates content of Leymus secalinus in a salinized grassland［J/OL］.Acta Agrestia Sinica（2023-07-05）［2023-08-15］..
14	祁瑜，黄永梅，王艳，等.施氮对几种草地植物生物量及其分配的影响［J］.生态学报，2011，31（18）：5121-5129.
	QI Y， HUANG Y M， WANG Y，et al.Biomass and its allocation of four grassland species under different nitrogen levels［J］.Acta Ecologica Sinica，2011，31（18）：5121-5129.
15	刘洋，彭云峰，门明新，等.青藏高原高寒草原根系动态对氮添加的响应及其调控因素［J］.应用生态学报，2021，32（9）：3119-3126.
	LIU Y， PENG Y F， MEN M X，et al.Response of root dynamics to nitrogen addition and the influencing factors in a Tibetan alpine steppe，China［J］.Chinese Journal of Applied Ecology，2021，32（9）：3119-3126.
16	王娇，关欣，张伟东，等.杉木幼苗生物量分配格局对氮添加的响应［J］.植物生态学报，2021，45（11）：1231-1240.
	WANG J， GUAN X， ZHANG W D，et al.Responses of biomass allocation patterns to nitrogen addition of Cunninghamia lanceolata seedlings［J］.Chinese Journal of Plant Ecology，2021，45（11）：1231-1240.
17	史顺增，熊德成，冯建新，等.模拟氮沉降对杉木幼苗细根的生理生态影响［J］.生态学报，2017，37（1）：74-83.
	SHI S Z， XIONG D C， FENG J X，et al.Ecophysiological effects of simulated nitrogen deposition on fine roots of Chinese fir （Cunninghamia lanceolata） seedlings［J］.Acta Ecologica Sinica，2017，37（1）：74-83.
18	冯瑜，张国防，李左荣.模拟不同施肥处理对芳樟树高和地径的影响［J］.中南林业科技大学学报，2015，35（6）：34-39.
	FENG Y， ZHANG G F， LI Z R.Effects of simulated different fertilization treatments on tree height and base diameter of Cinnamomum camphora var.1inaloolifera Fujita［J］.Journal of Central South University of Forestry & Technology，2015，35（6）：34-39.
19	于丽敏，王传宽，王兴昌.三种温带树种非结构性碳水化合物的分配［J］.植物生态学报，2011，35（12）：1245-1255.
	YU L M， WANG C K， WANG X C.Allocation of nonstructural carbohydrates for three temperate tree species in northeast China［J］.Chinese Journal of Plant Ecology，2011，35（12）：1245-1255.
20	李合生.植物生理生化实验原理和技术［M］.北京：高等教育出版社，2000：194-205.
	LI H S.Principles and techniques of plant physiological biochemical experiment［M］.Beijing：Higher Education Press，2000：194-205.
21	LIU M H， CHEN S X， KORPELAINEN H，et al.Nitrogen addition affects eco-physiological interactions between two tree species dominating in subtropical forests［J］.Plant Physiology and Biochemistry，2021，162：150-160.
22	李树萍，董琼，李世民，等.树番茄幼苗生长及氮积累与分配对光照和氮素添加的响应［J］.江西农业大学学报，2023，45（1）：156-168.
	LI S P， DONG Q， LI S M，et al.Responses of Cyphomandra betacea Sendt.seedling growth and nitrogen accumulation and allocation to light and nitrogen addition［J］.Acta Agriculturae Universitis Jiangxiensis，2023，45（1）：156-168.
23	齐成媚，肖丽酾，刘辉龙，等.模拟氮沉降下假臭草的生长特征［J］.热带作物学报，2023，44（3）：589-596.
	QI C M， XIAO L S， LIU H L，et al.Growth characteristics of Praxelis clematidea （Griseb.） under simulated nitrogen deposition［J］.Chinese Journal of Tropical Crops，2023，44（3）：589-596.
24	WANG M， SHI S， LIN F，et al.Effects of soil water and nitrogen on growth and photosynthetic response of Manchurian ash （Fraxinus mandshurica） seedlings in northeastern China［J］.PLoS One，2012，7（2）：e30754.
25	毛晋花，邢亚娟，闫国永，等.陆生植物生物量分配对模拟氮沉降响应的Meta分析［J］.生态学报，2018，38（9）：3183-3194.
	MAO J H， XING Y J， YAN G Y，et al.A Meta-analysis of the response of terrestrial plant biomass allocation to simulated N deposition［J］.Acta Ecologica Sinica，2018，38（9）：3183-3194.
26	WANG R Z， CAVAGNARO T R， JIANG Y，et al.Carbon allocation to the rhizosphere is affected by drought and nitrogen addition［J］.Journal of Ecology，2021，109（10）：3699-3709.
27	王立冬，梁海燕，王燕茹，等.不同氮素形态与配比对杉木和木荷幼苗光合特性及生长的影响［J］.四川农业大学学报，2023，41（2）：217-224.
	WANG L D， LIANG H Y， WANG Y R，et al.Effects of different nitrogen forms and ratios on photosynthetic characteristics and growth in Cunninghamia lanceolata and Schima superba seedlings［J］.Journal of Sichuan Agricultural University，2023，41（2）：217-224.
28	高歌，葛晓改，周君刚，等.施氮和干旱对杉木和青冈幼苗生物量及根系形态的影响［J］.生态环境学报，2022，31（12）：2292-2301.
	GAO G， GE X G， ZHOU J G，et al.Effect of drought stress and nitrogen addition on the biomass and root morphology of Cunninghamia lanceolata and Cyclobalanopsis glauca seedlings［J］.Ecology and Environmental Sciences，2022，31（12）：2292-2301.
29	代泽成，刘月秀，党宁，等.长期氮水添加对温带草原土壤化学性质和微生物学特性的短期遗留效应［J］.应用生态学报，2023，34（7）：1834-1844.
	DAI Z C， LIU Y X， DANG N，et al.Short-term legacy effects of long-term nitrogen and water addition on soil chemical properties and micro-bial characteristics in a temperate grassland［J］.Chinese Journal of Applied Ecology，2023，34（7）：1834-1844.
30	吴茜，丁佳，闫慧，等.模拟降水变化和土壤施氮对浙江古田山5个树种幼苗生长和生物量的影响［J］.植物生态学报，2011，35（3）：256-267.
	WU Q， DING J， YAN H，et al.Effects of simulated precipitation and nitrogen addition on seedling growth and biomass in five tree species in Gutian Mountain，Zhejiang Province，China［J］.Chinese Journal of Plant Ecology，2011，35（3）：256-267.
31	王晓荣，潘磊，唐万鹏，等.氮素添加对中亚热带栎属不同树种幼苗生长及生物量分配的短期影响［J］.东北林业大学学报，2014，42（6）：24-28.
	WANG X R， PAN L， TANG W P，et al.Short-term effects of nitrogen addition on seedling growth and biomass allocation with different tree species of Quercus in the mid-subtropics of China［J］.Journal of Northeast Forestry University，2014，42（6）：24-28.
32	PERCHLIK M， TEGEDER M.Leaf amino acid supply affects photosynthetic and plant nitrogen use efficiency under nitrogen stress［J］.Plant Physiology，2018，178（1）：174-188.
33	SHI F X， SONG C C， ZHANG X H，et al.Plant zonation patterns reflected by the differences in plant growth，biomass partitioning and root traits along a water level gradient among four common vascular plants in freshwater marshes of the Sanjiang Plain，northeast China［J］.Ecological Engineering，2015，81：158-164.
34	高剑飞，韩飞，张家铱，等.根性状的共变性和可塑性驱动不同水稻基因型对氮环境的适应性［J］.植物营养与肥料学报，2022，28（4）：611-621.
	GAO J F， HAN F， ZHANG J Y，et al.The covariation and plasticity of root traits drive different rice genotypes to adapt to the nitrogen environment［J］.Journal of Plant Nutrition and Fertilizers，2022，28（4）：611-621.
35	贾林巧，陈光水，张礼宏，等.罗浮栲和米槠细根形态功能性状对短期氮添加的可塑性响应［J］.应用生态学报，2019，30（12）：4003-4011.
	JIA L Q， CHEN G S， CHEN L H，et al.Plastic responses of fine root morphological traits of Castanopsis fabri and Castanopsis carlesii to short-term nitrogen addition［J］.Chinese Journal of Applied Ecology，2019，30（12）：4003-4011.
36	孟秋实，秦倩倩，刘艳红.氮添加对东北红豆杉幼苗生长发育及生理特征的影响［J］.生态学杂志，2022，41（12）：2325-2334.
	MENG Q S， QIN Q Q， LIU Y H.Effects of nitrogen addition on growth，development，and physiological characteristics of Taxus cuspidata seedlings［J］.Chinese Journal of Ecology，2022，41（12）：2325-2334.
37	郝龙飞，郝文颖，刘婷岩，等.氮添加及接种处理对1年生樟子松苗木根系形态及养分含量的影响［J］.北京林业大学学报，2021，43（4）：1-7.
	HAO L F， HAO W Y， LIU Y T，et al.Responses of root morphology and nutrient content of Pinus sylvestris var.mongolica seedlings to nitrogen addition and inoculation treatments［J］.Journal of Beijing Forestry University，2021，43（4）：1-7.
38	李紫帅，柳文杰，张阗真，等.不同施氮方式及施氮量对砂生槐生长及生根的影响［J］.东北林业大学学报，2023，51（1）：22-26.
	LI Z S， LIU W J， ZHANG T Z，et al.Effects of different nitrogen application methods and amounts on the growth and rooting of Sophora moorcroftiana ［J］.Journal of Northeast Forestry University，2023，51（1）：22-26.
39	王凯，沈潮，曹鹏，等.沙地樟子松幼苗干旱致死过程中非结构性碳水化合物的变化［J］.应用生态学报，2018，29（11）：3513-3520.
	WANG K， SHEN C， CAO P，et al.Changes of non-structural carbohydrates of Pinus sylvestris var.mongolica seedlings in the process of drought-induced mortality［J］.Chinese Journal of Applied Ecology，2018，29（11）：3513-3520.
40	章异平，师志强，竹磊，等.秦岭东段不同海拔栓皮栎粗根非结构性碳水化合物含量的季节动态［J］.生态学杂志，2021，40（3）：712-720.
	ZHANG Y P， SHI Z Q， ZHU L，et al.Seasonal variations of non-structural carbohydrate contents in coarse roots of Quercus variabilis Blume at different altitudes in the eastern Qinling Mountain［J］.Chinese Journal of Ecology，2021，40（3）：712-720.
41	祁琳，郭龙梅，刘尤德，等.刺槐幼苗非结构性碳水化合物对NaCl胁迫的动态响应［J］.林业科学，2022，58（1）：32-42.
	QI L， GUO L M， LIU Y D，et al.Dynamic responses of non-structural carbohydrates in Robinia pseudoacacia seedlings to NaCl stress［J］.Scientia Silvae Sinicae，2022，58（1）：32-42.
42	吕茹冰，杜莹，鲍永新，等.氮沉降对毛竹非结构性碳组成与分配的影响［J］.生态学杂志，2017，36（3）：584-591.
	LÜ R B， DU Y， BAO Y X，et al.Effects of simulated nitrogen deposition on the composition and allocation of non-structural carbohydrates of Phyllostachys edulis ［J］.Chinese Journal of Ecology，2017，36（3）：584-591.
43	XIE Y B， WAQAS M， KHAN M U，et al.Overexpression of the rice gene Lsi1 （low silicon gene 1） enhances plant-microbe interactions that result in improved chilling tolerance［J］.Plant Growth Regulation，2022，98（1）：525-538.
44	王宣璎，张翼，范秀华.氮沉降对蒙古栎幼苗生长及光合特性的影响［J］.森林与环境学报，2023，43（3）：280-286.
	WANG X Y， ZHANG Y， FAN X H.Effects of simulated nitrogen deposition on growth and photosynthetic characteristics of Quercus mongolica seedlings［J］.Journal of Forest and Environment，2023，43（3）：280-286.
45	闫道良，袁虎威，史骥清，等.施氮对铁皮石斛氮磷及非结构性碳水化合物含量的影响［J］.核农学报，2020，34（7）：1561-1567.
	YAN D L， YUAN H W， SHI J Q，et al.Effects of nitrogen application on contents of nitrogen，phosphorus and non-structural carbohydrate in Dendrobium officinale ［J］.Journal of Nuclear Agricultural Sciences，2020，34（7）：1561-1567.

处理 Nitrogen treatments	叶生物量 Leaf biomass/g	茎生物量 Stem biomass/g	根生物量 Root biomass/g		冠层生物量 Canopy biomass/g	总生物量 Total biomass/g	根冠比 Root shoot ratio	叶茎比 Leaf stem ratio
处理 Nitrogen treatments	叶生物量 Leaf biomass/g	茎生物量 Stem biomass/g	粗根生物量 Coarse root biomass	细根生物量 Fine root biomass	冠层生物量 Canopy biomass/g	总生物量 Total biomass/g	根冠比 Root shoot ratio	叶茎比 Leaf stem ratio
CK	1.80±0.21c	4.75±0.77b	2.66±0.44b	1.63±0.19b	6.55±0.90c	10.83±1.35b	0.60±0.07a	0.38±0.05b
N1	8.87±1.06a	8.88±0.86a	5.75±0.80a	3.25±0.32a	17.75±1.74a	26.75±2.62a	0.55±0.04ab	1.00±0.10a
N2	6.13±0.44b	5.15±0.23b	2.93±0.25b	1.75±0.14b	11.28±1.09b	15.96±0.95b	0.41±0.02b	1.19±0.06a

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[2]	Kenian LINGHU, Shu WANG. Responses of Biomass Allocation to Population Density and Soil Water in Abutilon theophrasti at Different Growth Stages [J]. Bulletin of Botanical Research, 2023, 43(2): 272-280.
[3]	Chunyan WEI, Yueling LI, Zexin JIN, Guangyu LUO, Chao CHEN, Fangquan SHAN. Effects of Shading on Photosynthetic Characteristics and Non-structural Carbohydrate Content of Heptacodium miconioides Seedlings [J]. Bulletin of Botanical Research, 2022, 42(6): 1096-1105.
[4]	Meng-Tian DU, Bo-Yi WANG, Jing-Hang LI, Ming-Yuan ZHAO, Xiu-Wei WANG. Differences and Seasonal Dynamics of Soluble Sugar and Starch Concentrations in Fine Roots with Different Root Orders of Larix gmelinii [J]. Bulletin of Botanical Research, 2021, 41(4): 491-495.
[5]	SHANG Kan-Kan, ZHANG Xi-Jin, SONG Kun. Variation of Stem-leaf Size Relationship of Woody Plants among Different LifeForms in Shanghai Chenshan Botanical Garden [J]. Bulletin of Botanical Research, 2020, 40(5): 641-647.
[6]	XIAO Zu-Fei, WANG Ling-Ling, CAO Lu-Yao, LIAO Wen-Xuan, JIN Zhi-Nong, LI Feng, Lü Xiong-Wei, ZHANG Bei-Hong, ZHAO Jiao. Tissue Culture Technology of Stem Segment of Cinnamomum bodinieri var. citralifera [J]. Bulletin of Botanical Research, 2020, 40(2): 196-201.
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[8]	WANG Zong-Yan, WANG Kai, JIANG Tao, CAO Peng, SUN Ju, WANG Dao-Han. Staged Responses of Non-structural Carbohydrates of Pinus tabulaeformis Seedlings to Drought Stress [J]. Bulletin of Botanical Research, 2018, 38(3): 460-466.
[9]	MA Han-Ze, WANG Cheng-Zhong, LI Ding, FAN Guo-Zhen, HU Yong-Hong, SONG Zhi-Ping. Effects of Growth Environment and Tree Age on Biomass Allocation within Fruit Twigs of Fengdan(Paeonia ostii) [J]. Bulletin of Botanical Research, 2018, 38(2): 201-211.
[10]	YIN Dong-Sheng, SHEN Hai-Long, WEI Xiao-Hui. Effects of Shading on Physocarpus amurensis Seedlings Photosynthetic Ability and Carbohydrate Accumulation [J]. Bulletin of Botanical Research, 2017, 37(6): 841-847.
[11]	GUO Hai-Xia, XU Bo, SHI Fu-Sun, WU Yan. Effects of Shading and Nitrogen Addition on the Growth and C-N Balance of Fritillaria unibracteata [J]. Bulletin of Botanical Research, 2017, 37(5): 738-743.
[12]	SHU Wen-Jiang, CHAI Sheng-Feng, LI Zhao-Hai, FAN Jin-Shun, TANG Jian-Min, ZHU Shu-Fang, HE Zhi-Hong. Effects of Different Light Intensities on the Growth and Photosynthesis of Symplocos tetragona Seedlings [J]. Bulletin of Botanical Research, 2017, 37(4): 556-562.
[13]	ZHAO Jing, WANG Xi-Yuan. Allocation Patterns and Allometric Relationships between Above- and Below-ground Biomass of Three Life-form Herbaceous Plants in the Gurbantünggüt Desert [J]. Bulletin of Botanical Research, 2017, 37(2): 304-311.
[14]	LI Wen；ZHANG Cheng；WANG Qing-Cheng；HAO Long-Fei；LIU Shuang. Effect of Exponential Fertilization on Growth Dynamic,Biomass Allocation and Photosynthesis of Betula platyphylla Bare-rooted Seedlings [J]. Bulletin of Botanical Research, 2015, 35(3): 391-396.
[15]	LI Zhi-Bo;LI Ping-Heng;WANG Quan;;XU Lu;. Allometric Characteristics of Terminal Twigs of Tamarix ramosissima* and Haloxylon ammodendron [J]. Bulletin of Botanical Research, 2013, 33(3): 274-281.