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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (3): 436-440.doi: 10.7525/j.issn.1673-5102.2021.03.014

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Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC

Min CHEN1, Meng-Fei TIAN1, Chang-Yin FENG1, Xian-Ming MENG1, Meng LUO2, Fan-Song MU1()   

  1. 1.College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040
    2.College of Ecology&Environment,Hainan Tropical Ocean University,Sanya 572022
  • Received:2020-03-17 Online:2021-05-20 Published:2021-03-24
  • Contact: Fan-Song MU E-mail:fansong8867@nefu.edu.cn
  • About author:CHEN Min(1992—),male,master,mainly engaged in plant active ingredient research.
  • Supported by:
    Fundamental Research Funds for the Central University(2572018BU04)


In order to establish an HPLC method for the simultaneous determination of baschnaloside and rossicaside B in Boschniakia rossica dry whole, a chromatographic column: Klimail 100-5 C18 column(250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile as mobile phase A, 0.5% formic acid aqueous solution as mobile phase B, gradient elution conditions: 0?12 min, 15% A; 12?30 min, 15%?20% A; 30?40 min, 20%?25% A; 40?45 min, 25%?30% A; 45?60 min, 30%?100% A; flow rate: 1.0 mL·min-1; detection wavelength: 260 nm. Column temperature: 30℃. Injection volume: 20 μL. The linear range of naringin of baschnaloside was 4.688?150 μg·g-1R2=0.999 5); the linear range of rossicaside B was 3.438?110 μg·g-1R2=0.999 1); the average recoveries were 97.86% and 96.55%, and RSDs were 1.01 and 1.23(n=9), respectively. A high-performance liquid chromatography method was developed for the simultaneous determination of two components, baschnaloside and rossicaside B in B.rossica. The methodological verification results show that the method is simple, fast, and reproducible, and can be used to determine the contents of baschnaloside and rossicaside B in B.rossica.

Key words: Boschniakia rossica, HPLC, baschnaloside, rossicaside B

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