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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (6): 758-762.doi: 10.7525/j.issn.1673-5102.2010.06.024

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Inhibitory Effect of Algae Growth and HPLC Analysis of Allelochemicals from Stephania tetrandra

CHAI Min-Wei;SHI Fu-Chen*;MA Yan   

  1. College of Life Sciences,Nankai University,Tianjin 300071
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Contact: SHI Fu-Chen
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Abstract: Aqueous extracts of 12 kinds of medicinal plants were obtained by soak extraction, and their inhibitory effects on algae growth were studied. The results showed that: when the relative concentration was 1 g·L-1, aqueous extracts of Stephania tetrandra had the strongest inhibitory effect on the growth of Chlorella pyrenoidosa, and the maximum specific growth rate was -0.28 d-1. The methanol extract of S.tetrandra had significant and long-lasting inhibitory effect on the growth of C.pyrenoidosa and the lowest effective concentration in inhibiting algae was 30 mg·L-1.The identification results of HPLC showed that: the main constituents in the extracts were alkaloids such as fangchinoline and tetrandrine, etc.S.tetrandra had great prospect of application in controlling water bloom.

Key words: medicinal plant, water bloom, Stephania tetrandra, Chlorella pyrenoidosa, HPLC

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