Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (3): 321-328.doi: 10.7525/j.issn.1673-5102.2021.03.001
• Research report • Next Articles
Hong-Mei TIAN, Xiao GUO, Kui-Ling WANG, Ying-Kun SUN()
Received:
2019-11-25
Online:
2021-05-20
Published:
2021-03-24
Contact:
Ying-Kun SUN
E-mail:sunyk678@163.com
About author:
TIAN Hong-Mei(1994-),female,master,major in the landscape plant genetic breeding.
Supported by:
CLC Number:
Hong-Mei TIAN, Xiao GUO, Kui-Ling WANG, Ying-Kun SUN. Plant Regeneration System from Cotyledon of Camellia japonica (Naidong)[J]. Bulletin of Botanical Research, 2021, 41(3): 321-328.
Add to citation manager EndNote|Ris|BibTeX
URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.03.001
Table 2
Effects of different hormone combinations on cotyledon callus growth
实验编号 Test No. | 激素配比 Hormone combination | 增殖倍数 Multiplication rate | 生长状态 Growing status | |
---|---|---|---|---|
NAA(mg·L-1) | 6-BA(mg·L-1) | |||
1 | 0.1 | 2.0 | 2.31 | 黄绿色,微泛红,颗粒细小紧致 Yellow-green and pink,creamy and compact |
2 | 0.1 | 1.5 | 2.77 | 浅黄绿色,少数微红,比较水润 Pale greenish-yellow,a little callus was pink,moist |
3 | 0.1 | 1.0 | 1.15 | 30 %的愈伤组织为黄绿色,其余为灰褐色 Thirty percent callus was yellow-green,the rest was beige |
4 | 0.1 | 0.5 | 2.42 | 表面干燥,颗粒状明显,生长量大 The surface was dry,granular,growth increment larger |
5 | 0.2 | 2.0 | 2.08 | 浅黄绿色,比较水润Pale greenish-yellow,moist |
6 | 0.2 | 1.5 | 1.77 | 黄绿色,比较水润Yellow-green,moist |
7 | 0.2 | 1.0 | 1.67 | 黄绿色,表面比较干燥Yellow-green,the surface was dry |
8 | 0.2 | 0.5 | 1.27 | 黄绿色,表面干燥,愈伤边缘略发黑 Yellow-green , the surface was dry,callus of peripheral was black |
9 | 0.3 | 2.0 | 1.85 | 微泛红,表面干燥,颗粒细小紧致 Pink,the surface was dry,creamy,creamy and compact |
10 | 0.3 | 1.5 | 2.67 | 黄绿色,表面稍微干燥Yellow-green,the surface was a little dry |
11 | 0.3 | 1.0 | 1.86 | 黄绿色,比较水润Yellow-green,moist |
12 | 0.3 | 0.5 | 1.91 | 黄绿色,比较水润Yellow-green,moist |
13 | 0.4 | 2.0 | 1.89 | 浅黄绿色,比较水润Pale greenish-yellow,moist |
14 | 0.4 | 1.5 | 3.29 | 愈伤组织为浅红色和黄绿色,6.67%的愈伤出现不均质颗粒 Yellow-green and lighter red,nodular of 6.67 % callus |
15 | 0.4 | 1.0 | 3.16 | 黄绿色,少数微微泛红,表面干燥 Yellow-green,a little callus was pink,the surface was dry |
16 | 0.4 | 0.5 | 1.17 | 干燥,愈伤表面呈黑褐色Dry,the surface of callus was dark brown |
17 | 0.5 | 2.0 | 2.38 | 黄绿色,比较水润Yellow-green,moist |
18 | 0.5 | 1.5 | 4.06 | 黄绿色,微微泛红,20%的愈伤出现不均质颗粒 Yellow-green and pink,nodular of 20% callus |
19 | 0.5 | 1.0 | 2.35 | 黄绿色,表面稍微干燥yellow-green,the surface was a little dry |
20 | 0.5 | 0.5 | 1.83 | 愈伤微微泛红,较水润Pink and moist |
Table3
Effects of different hormone combinations on differentiation of C. japonica (Naidong)
编号 Test No. | 不同激素配比 Different hormone combination(mg·L-1) | 丛生芽诱导率 The rate of differentiation(%) | 愈伤组织生长状态 Growing status of callus |
---|---|---|---|
1 | 0.25IBA+106-BA | 0 | 愈伤组织呈灰褐色Beige |
2 | 0.5IBA+106-BA | 0 | 与培养基接触的愈伤组织呈黑褐色,质地松散 The callus contacted to the medium was dark brown and loose structure |
3 | 0.25NAA+106-BA | 1.50 | 黄绿色,质地紧实,比较水润Yellow-green, compact, moist |
4 | 0.5NAA+106-BA | 0.89 | 浅黄绿色,较干燥,质地紧实Pale greenish-yellow,dry,compact |
5 | 0.75NAA+106-BA | 3.90 | 黄绿色,质地紧实,比较水润The callus was yellow-green,moist,compact |
6 | 1NAA+106-BA | 10.70 | 愈伤组织黄绿色,质地紧实且水润有光泽Yellow-green,compact and moist |
Table 4
Effects of media types on differentiation of C. japonica(Naidong)
编号 Test No. | 培养基种类 Media types | 生根率 Rate of rooting(%) | 生长状态 Growing status of roots |
---|---|---|---|
1 | MW | 0 | 茎段变红,膨大 The stem segments changed to red with swollen |
2 | 1/2MS | 40 | 茎段变红,膨大,茎部有不定根和少量愈伤组织的产生 The stem segments were red and swollen with adventitious roots and a little callus grow out |
1 | 王奎玲,牟少华,刘庆超,等.部分耐冬山茶栽培品种的AFLP分析[J].中国农业科学,2011,44(3):651-656. |
Wang K L,Mou S H,Liu Q C,et al.Studies on the Genetic Diversity of Some Camellia japonica(Naidong) Cultivars Based on AFLP Markers[J].Scientia Agricultura Sinica,2011,44(3):651-656. | |
2 | 王奎玲.耐冬山茶种质资源研究[D].北京林业大学,2006. |
Wang K L.Studies on the germplasm resources of Naidong Shancha(Camellia japonica L.)[D].Beijing Forestry University,2006. | |
3 | 陈艳丽.耐冬山茶组织培养再生体系的研究[D].山东:莱阳农学院,2006:1-52. |
Chen Y L.Studies on Regeneration System in Tissue Culture of Naidong Shancha(Camelliajaponica L.).Qingdao Agricultural University,2006:1-52. | |
4 | 董慧慧,王奎玲,薛秋华,等.耐冬山茶愈伤组织诱导研究[J].福建林业科技,2007,34(3):135-139. |
Dong H H,Wang K L,Xue Q H,et al.The preliminary studies on the callus induction of Camellia japonica “Naidong”[J].Jour of Fujian Forestry Sci and Tech,2007,34(3):135-139. | |
5 | 杨凯.耐冬山茶胚状体和胚性愈伤组织诱导研究[J].青岛农业大学学报:自然科学版,2013,30(3):184-187+199. |
Yang K.Induction of Somatic Embryogenesis and Embryogenic Callus from Camellia japonica[J].Journal of Qingdao Agricultural University:Natural Science,2013,30(3):184-187+199. | |
6 | 吴斌,李纪元,范正琪,等.山茶‘耐冬’愈伤组织诱导与植株再生[J].植物生理学报,2013,49(4):343-346. |
Wu B,Li J Y,Fan Z Q,et al.Callus Induction and Plant Regeneration of Camellia japonica L.‘Naidong’[J].Plant Physiology Journal,2013,49(4):343-346. | |
7 | 吴斌.山茶‘耐冬’愈伤组织诱导与植株再生的研究[D].南充:四川师范大学,2013. |
Wu B.Study on Callus Induction and Plant Regeneration of Camellia japonica L.‘Naidong’[D].Nanchong:Sichuan Normal University,2013. | |
8 | 周炳庚.应用椰子汁做培养基的初步研究[J].广东医学:现代医学版,1965(S3):186-188. |
Zhou B G.Preliminary Studies on Medium of coconut milk[J].Guangdong medicine:Modern Medicine Edition,1965(S3):186-188. | |
9 | 李青林,邹永田,刘广林,等.木本观赏植物组织培养技术[J].河北农业科学,2010,14(6):38-41+51. |
Li Q L,Zou Y T,Liu G L,et al.Tissue Culture Technology of Woody ornamental plants[J].Journal of Hebei Agricultural Sciences,2010,14(6):38-41+51. | |
10 | 曹昆,李霞.木本植物组织培养不定芽诱导研究进展[J].江苏林业科技,2008(5):43-48. |
Cao K,Li X.Progress on induction of adventitious bud in tissue culture of woody plants[J].Journal of Jiangsu Forestry Science & Technology,2008(5):43-48. | |
11 | Ledbetter D I,Preece J E.Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr.leaf explants[J].Scientia Horticulturae(Amsterdam),2004,101(1-2):0-126. |
12 | 李代丽,康向阳.植物愈伤组织培养中内外源激素效应的研究现状与展望[J].生物技术通讯,2007,18(3):546-548. |
Li D L,Kang X Y.The Status and Prospect of Exodogenous Hormone and Endogenous Hormone Impact in Plant Callus Tissue Culture[J].Letters in Biotechnology,2007,18(3):546-548. | |
13 | 王秀红.水稻不同外植体的组织培养能力及其内源激素分析[J].四川农业大学,2004,37(12):1819-1823. |
Wang X H.Analysis of Tissue Culture Abilities and Endogenous Hormones from Different Explants in Rice(Oryza sativa L.)[J].Sichuan Agricultural University,2004,37(12):1819-1823. | |
14 | 徐华松,徐九龙,黄学林.TDZ在植物组织培养中的作用[J].广西植物,1996(1):77-80. |
Xu H S,Xu J L,Huang X L.Effect of TDZ on Plant Tissue Culture[J].Guihaia,1996(1):77-80. | |
15 | Wang H C,Chen J T,Wu S P,et al.Plant regeneration through shoot formation from callus of Areca catechu L.[J].Plant Cell Tissue and Organ Culture,2003,75(1):95-98. |
16 | 陈云风,张春荣,黄霞,等.TDZ对植物体细胞胚胎发生的作用[J].植物生理学通讯,2006,42(1):127-133. |
Chen Y F,Zhang C R,Huang X,et al.Effect of TDZ on Somatic Embryogenesis of Plant[J].Plant Physiology Journal,2006,42(1):127-133. | |
17 | 吉训志,秦晓威,胡丽松,等.木本植物组织培养[J].热带农业科学,2019,39(4):33-40. |
Ji X Z,Qin X W,Hu L S,et al.Current Research on Tissue Culture of Woody Plants[J].Chinese Journal of Tropical Agriculture,2019,39(4):33-40. | |
18 | 许益娟.茶树组织培养再生体系优化与遗传转化的研究[D].南京农业大学,2012. |
Xu Y J.Studies on Optimization of the Regeneration and Genetic Transformation in Camellia sinensis(L.) O.Kuntze[D].Nanjing Agricultural University,2012. | |
19 | Cao X,Hammerschlag F A,Douglass L.A Two-step Pretreatment Significantly Enhances Shoot Organogenesis from Leaf Explants of Highbush Blueberry cv.Bluecrop[J].HortScience:a publication of the American Society for Horticultural Science,2002,37(5). |
20 | 应承纪,周建葵.云南山茶花茎尖培养中多芽体的形成和生根的研究[J].实验生物学报,1997(1):1-8+10-11. |
Ying C J,Zhou J K.Studies on the formation and rooting of bud in the shoot tip culture of Camellia reticulata Lindl[J].Acta Biologiae Experimentalis Sinica,1997(1):1-8+10-11. | |
21 | Welander M.In vitro rooting of the apple rootstock M 26 in adult and juvenile growth phases and acclimatization of the plantlets[J].Physiologia Plantarum,2006,58(3):231-238. |
[1] | Zhuanzhuan JIANG, Li GONG, Yaling SONG. The Chloroplast Division Protein PARC6 Affected the Growth of Cotyledon and Leaf in Arabidopsis thaliana [J]. Bulletin of Botanical Research, 2023, 43(5): 700-710. |
[2] | Wenjie AN, Yinjie WANG, Qingquan LIU, Yongheng YANG, Ting ZHANG, Shaoru FAN, Yongxia ZHANG, Haiyan YUAN. Establishment of an Efficient Regeneration System in Iris lacteal var. chinensis with Immature Embryo [J]. Bulletin of Botanical Research, 2023, 43(3): 333-339. |
[3] | Yuhui REN, Shuai NIE, Chunxue PENG, Ling YANG, Hailong SHEN. Effects of Hormone Combinations, Carbon Source Type and Proliferation Cycle on Embryogenic Callus Proliferation of Pinus koraiensis [J]. Bulletin of Botanical Research, 2022, 42(4): 704-712. |
[4] | Qiyang Gao, Yulong Huang, Wenbing Guo, Fencheng Zhao, Yang Liu. Effects of Carbon Source and Other Factors on the Proliferation of Embryogenic Callus of Elite Clone of Pinus elliottii [J]. Bulletin of Botanical Research, 2022, 42(1): 21-28. |
[5] | YUAN Yun-Xiang. Callus induction and Plant Regeneration of Euonymus microcarpus(Oliv.) Spraggue [J]. Bulletin of Botanical Research, 2020, 40(5): 673-678. |
[6] | DU Yi-Ming, ZHONG Yuan, SHANG Hong-Qin, CHENG Fang-Yun. Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’ [J]. Bulletin of Botanical Research, 2020, 40(4): 514-522. |
[7] | ZHANG Jing, NIU Zhe, FAN Wei-Fang, GAO Peng-Fei, WU Jian-Hui. Establishing Tissue Culture and Regeneration System of Scutellaria regeliana [J]. Bulletin of Botanical Research, 2020, 40(1): 50-57. |
[8] | ZHANG Lu, PAN Yuan-Zhi, LIU Shi-Liang, CHEN Yuan, SHEN Li-Jia. Embryonic Callus Induction and Plant Regeneration of Lilium leucanthum [J]. Bulletin of Botanical Research, 2019, 39(3): 338-346. |
[9] | LIN Yun, BI Hai-Yan, LI Chao, YUN Ying-Xia. Correction of Typographical Errors in the Protologue of Eleven Taxa(Dicotyledon) in China [J]. Bulletin of Botanical Research, 2019, 39(2): 310-320. |
[10] | WANG Li-Na, WANG Yu-Cheng, YANG Chuan-Ping. The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar [J]. Bulletin of Botanical Research, 2017, 37(4): 542-548. |
[11] | GAO Jie1;WANG Yuan-Zhong2;HUANG Heng-Yu1*. Embryonic Callus Induction and Plant Regeneration of Lilium fargesii [J]. Bulletin of Botanical Research, 2016, 36(1): 52-57. |
[12] | LIN Yun1;LIU Wen-Qun1;WU Hui-Bing1;SUN Miao2;SUN Qian2;YANG Zhi-Rong2;LIN Qi2*. Lectotypifications of Sixteen Names in Dicotyledon [J]. Bulletin of Botanical Research, 2015, 35(6): 807-811. |
[13] | JIN Jian-Bang1,3;CHENG Long-Xia1,3;SHI Man1,3;ZHU Zun-Ling1,2,3*. Induction and Culture of Carpinus betulus Callus [J]. Bulletin of Botanical Research, 2015, 35(5): 684-691. |
[14] | HUANG Zhen1,2;LI Yun1,2;LI Yuan1,2;WANG Pei-Qi1,2;KANG Xiang-Yang1,2*. Establishment and Regeneration of Suspension Cell Lines in Hybirds of (Populus pseudo-simonii×P.nigra ‘Zhenyin3#’)×P.×beijingensis [J]. Bulletin of Botanical Research, 2015, 35(5): 696-703. |
[15] | WANG Ling-Li1,2;LIU Wen-Zhe2*. Induction and Effects of Elicitor on Camptothecin Content in Camptotheca acuminata Callus [J]. Bulletin of Botanical Research, 2015, 35(5): 704-709. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||