Subcellular Localization of the Protein Coded by the UDP-Glucose Dehydrogenase Gene from Paper Mulberry and Functional of Its Promoter 5′-end Deletion Fragment

doi:10.7525/j.issn.1673-5102.2020.06.016

Abstract

Abstract:

We analyzed the function of cis-acting elements of UDP-Glucose Dehydrogenase Gene from Paper Mulberry（DDBJ， BpUGDH accession No.LC457701）promoter， the five different lengths of BpUGDH promoter 5'-terminal deletion fragment were ligated with the GUS gene by using 5'terminal deletion and homologous recombination techniques， and conducted through heterologous expression in Nicotiana benthamiana by agro-infiltration method. In order to locate the specific location of the protein encoded by BpUGDH gene in cells， subcellular localization of BpUGDH protein was carried out by using the fusion target gene of GFP reporter gene. The results show that the sequences within -244 bp can mediate the induction of GUS gene expression， and the region between -973， -465， -355， -281， -244 bp of the BpUGDH promoter were crucial for regulating the promoter activity. Furthermore， BpUGDH was located in chloroplast.

Key words: Cis-acting element, BpUGDH, subcellular localization, paper mulberry, 5'-end deletion analysis method, transient expression

CLC Number:

Q943.2

Ren-Hua JI, Wen-Bo ZHANG, Xiao-Fei LIN, Ying-Liang BAO, Ri-Ge-Le TE, Hui-Ga BAO, Shu-Lan BAI. Subcellular Localization of the Protein Coded by the UDP-Glucose Dehydrogenase Gene from Paper Mulberry and Functional of Its Promoter 5′-end Deletion Fragment[J]. Bulletin of Botanical Research, 2020, 40(6): 932-942.

Figures/Tables 7

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引物Primer	序列Sequences(5'-3')
PB2-ETH（-973）-F2	GCCCAACGTTCTCGAGTTTTTCGATTTTCTTCGGTTTCCGAGA
PB3-MeJA（-465）-F3	GCCCAACGTTCTCGAGTTTTTGCTGATGTGTTAGTCTCTCTTAC
PB4-低温（-355）-F4	GCCCAACGTTCTCGAGTTTTTGAATTCTGTGAAACTCGGAT
PB5-IAA（-281）-F5	GCCCAACGTTCTCGAGTTTTTGAGGTTACTCTTAGTTCTCTA
PB6-GA（-244）-F6	GCCCAACGTTCTCGAGTTTTTGATGGCAAGAAAAGGATTGGAGG
BpUGDH-Xbal-R	CCCGGGTGGATCCAAGGCCTTCTTTGCTCCTTTATCACTCTAT

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