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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (6): 932-942.doi: 10.7525/j.issn.1673-5102.2020.06.016

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Subcellular Localization of the Protein Coded by the UDP-Glucose Dehydrogenase Gene from Paper Mulberry and Functional of Its Promoter 5′-end Deletion Fragment

Ren-Hua JI1, Wen-Bo ZHANG1, Xiao-Fei LIN2, Ying-Liang BAO1, Ri-Ge-Le TE1, Hui-Ga BAO3, Shu-Lan BAI1()   

  1. 1.Forestry College,Inner Mongolia Agricultural University,Hohhot 010019
    2.College of Life Science,Inner Mongolia University,Hohhot 010021
    3.Wetlands Authority of Wuhai,Wuhai 016000
  • Received:2019-12-17 Online:2020-11-20 Published:2020-11-04
  • Contact: Shu-Lan BAI E-mail:baishulan2004@163.com
  • About author:JI Ren-Hua(1986—),female,Ph.D.,majoring in the forest biotechnology.
  • Supported by:
    National Natural Science Foundation of China(31660213);Autonomous Region Graduate Research Innovation Fundation of Inner Mongolia(B20171012913)


We analyzed the function of cis-acting elements of UDP-Glucose Dehydrogenase Gene from Paper Mulberry(DDBJ, BpUGDH accession No.LC457701)promoter, the five different lengths of BpUGDH promoter 5'-terminal deletion fragment were ligated with the GUS gene by using 5'terminal deletion and homologous recombination techniques, and conducted through heterologous expression in Nicotiana benthamiana by agro-infiltration method. In order to locate the specific location of the protein encoded by BpUGDH gene in cells, subcellular localization of BpUGDH protein was carried out by using the fusion target gene of GFP reporter gene. The results show that the sequences within -244 bp can mediate the induction of GUS gene expression, and the region between -973, -465, -355, -281, -244 bp of the BpUGDH promoter were crucial for regulating the promoter activity. Furthermore, BpUGDH was located in chloroplast.

Key words: Cis-acting element, BpUGDH, subcellular localization, paper mulberry, 5'-end deletion analysis method, transient expression

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