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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (3): 417-422.doi: 10.7525/j.issn.1673-5102.2014.03.020

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Critical Cis-acting Element in the Promoter of the Stress-tolerant Gene AtRPK1

SHI Cui-Cui;LI Xiao-Xu;LI Jing-Lan;REN Ya-Nan;ZHANG Fu-Zhen;HUANG Zhan-Jing;GE Rong-Chao*   

  1. 1.College of Life Science,Hebei Normal University,Shijiazhuang 050016;2.No.1 High School of Yongnian,Handan 057150
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: GE Rong-Chao
  • Supported by:

Abstract: To identify the critical cis-acting element, the deletion analysis was performed using the promoter region of the stress resistance related gene AtRPK1 in Arabidopsis. By the 5′ terminal deletion methods, the 203, 316, 604, 809 bp promoter fragments were obtained and constructed into p1300-pro-GUS expression vector and transferred into Arabidopsis. By GUS staining detection of 809 bp length promoter transgenic Arabidopsis, it was found that the AtRPK1 gene was expressed in the leaves, stems, flowers and roots. However, the expression of AtRPK1 was stronger in meristem organization and vascular tissue. The detection results show that the region from the transcription start sites to the -114 section of AtRPK1 gene promoter contains the critical cisacting elements. After the stress of 200 mmol·L-1 NaCl, quantification detection results of β-glucuronidase activity showed that the GT-1 cis-element GAAAAA at -19 section may be directly related to the salt stress response.

Key words: AtRPK1, promoter, critical cis-acting element, salt-responsive element

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