Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (2): 185-193.doi: 10.7525/j.issn.1673-5102.2017.02.005

Previous Articles     Next Articles

The Research of Key Influence Factors of Rapid Culture of the Protonema of Didymodon ditrichoides

ZHAO Yang1,2, LI Xiao-Ming4, LI Ru-Xue3, WANG Chun3, BU Chong-Feng1,3   

  1. 1. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100;
    2. University of Chinese Academy of Sciences, Beijing 100049;
    3. Institute of Soil and Water Conservation, Northwest A & F University, Yangling 712100;
    4. College of Nature Resource and Environment, Northwest A & F University, Yangling 712100
  • Received:2016-11-03 Online:2017-03-15 Published:2017-03-11
  • Supported by:
    The Light of West China of Chinese Academy of Sciences(2014-91);The Fundamental Research Funds for the Central Universities (2014YQ006);The National Nature Science Foundation of China(41541008)

Abstract: In order to provide a rich source of mass vaccination for the field restoration of moss crust, we used two kinds of disinfection method, 0.1% NaClO(10, 15, 20, 30, 45, 60, 120 s) and 0.1% HgCl2(10, 15, 20, 30, 45, 60, 120 s), with Knop, MS and Hoagland as culture media, and set the pH values(5.5, 6, 6.5, 7, 7.5, 8, 8.5), through the single factor experiment design method, we determined the survival rate of the cormus of[Didymodon ditrichoides(Broth.)], protonema length and number of branches, thereby exploring key factors that affect the propagation of the protonema of D.ditrichoides. The results are as follows:(1) The disinfection method, culture medium and pH value have significant influence upon the survival rate of the cormus of D.ditrichoides, length of protonema and number of branches(P<0.05), in a descending order of disinfection method, culture medium, and pH value; (2) The most suitable disinfection method is 0.1% NaClO with a duration of 15-20 s; (3) The most suitable culture medium for the growth of the protonema of D.ditrichoides is Knop and Hoagland; (4) The most suitable pH value for the growth of protonema of D.ditrichoides is 7.5. Therefore, the best combination of factors for rapid cultivation of the protonema of D.ditrichoides is 0.1% NaClO disinfection 15-20 s+Hoagland/Knop+pH=7.5. Our experiment can be determined to shorten the growth cycle of D.ditrichoides and realize rapid propagation, thereby providing experience for the rapid culture and engineering application of moss crust.

Key words: Didymodon ditrichoides, protonema, disinfection method, culture medium, pH

CLC Number: