PCR-RFLP ANALYSIS ON CHLOROPLAST DNA OF ACTINIDIA

Abstract

Abstract: Twentyseven species and fifteen variations of Actinidia were investigated through PCR-RFLP analysis of two chloroplast DNA fragments, including rbcL gene and psbA gene. The two chloroplast gene fragments were digested by two restriction endonucleasea respectively, which yielded twentyfive restriction sites and twentyfour of them were polymorphic. Haplotype of Actinidia was geographically established. Pattern of phylogene in Actinidia and relations between some important species were explored. Genetic information of molecular systematics in Actinidia was expanded by above all.

Key words: Aceinidia, chloroplast DNA, phylogene

LI Jian-Zi, LI Si-Guang, LUO Yu-Ping, CHEN Shao-Feng. PCR-RFLP ANALYSIS ON CHLOROPLAST DNA OF ACTINIDIA[J]. Bulletin of Botanical Research, 2003, 23(3): 328-333.

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PCR-RFLP ANALYSIS ON CHLOROPLAST DNA OF ACTINIDIA

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