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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (1): 69-73.doi: 10.7525/j.issn.1673-5102.2005.01.021

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Construction and analysis of Tamarix hispida suppression subtractive hybridization library under drought stress

LIU Gui-Feng1,2, HOU Ying-Jie2, WANG Yu-Cheng2, CHU Yan-Guang2   

  1. 1. School of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083;
    2. Forest College Northeast Forestry University, Harbin 150040
  • Received:2004-07-27 Online:2005-03-15 Published:2016-06-14

Abstract: Using cDNA from Tamarix hispida roots treated with drought stress as tester and cDNA from Tamarix hispida roots in normal growth as driver, suppression subtractive hybridization (SSH) was employed to construct cDNA subtracted library. In the library, the rate of recombination was 95%, the size of inserts was 250~600 bp. 17 drought stress-associated genes were obtained by DNA sequencing the positive clones picked randomly, such asMn-SOD myb-related protein zinc finger protein, etc. These genes included osmotic regulator signal component regulatory protein and antioxidant enzyme. GenBank had accepted all the sequences. The research had established a basis for cloning stress-resistance genes and further studying genes expression in Tamarix hispida roots under drought stress.

Key words: drought stress, Tamarix hispida, suppression subtractive hybridization, expressed sequence tags