Microwave-assisted Polysaccharide Extraction of Aralia chinensis L. Root and Composition Determination of Monosaccharide

doi:10.7525/j.issn.1673-5102.2015.05.023

Abstract

Abstract: We extracted the Aralia chinensis polysaccharide(ACP) from A.chinensis L. root by microwave-assisted technique. With the single factor experiment, we optimized four factors affecting the yield of A.chinensis root including time, temperature, microwave power and solid-liquid ratio by using orthogonal design method. The optimum extracting conditions for polysaccharide yield of 15.189% was under the ratio of solid to liquid 1∶30 (g·mL^-1), 40 min, 50℃, and microwave power of 400 W. Polysaccharides were deproteinized by Sevage method after dialysis treatment, and A.chinensis root had infrared spectral scanning polysaccharide characteristic absorption peaks. Ee measured the molecular weight of A.chinensis root polysaccharides by using SEC-HPLC and LLS: Mn=4.8×10⁵ g·mol^-1. A.chinensis root hydrolysis was derivatized by gas chromatography-mass spectrometry (GC-MS) analysis, and the polysaccharide of A.chinensis were composed of D-arabinose, D-glucose and D-galactose, with the molar ratio of 4.67∶76.44∶18.18.

Key words: microwave-assisted extraction, Aralia chinensis L. polysaccharide, orthogonal test, molecular weight, monosaccharide composition

CLC Number:

WANG Yi-Feng；HOU Hong-Hong；JIN Jie；ZHAO Bo；CAO Jia-Hao；LI Xiao-Jiao. Microwave-assisted Polysaccharide Extraction of Aralia chinensis L. Root and Composition Determination of Monosaccharide[J]. Bulletin of Botanical Research, 2015, 35(5): 779-785.

References

1.中国科学院中国植物志编辑委员会.中国植物志:第54卷［M］.北京:科学出版社,1978:159-160.

2.王忠壮,胡晋红.中国楤木属植物的生物学研究及应用［M］.上海:第二军医大学出版社,2001:8.

3.姜鸑,姜新.中国楤木属植物研究现状与展望［J］.河北农业科学,2008,12(5):87-90.

4.郑玲玲,装凌鹏.中国楤木属植物研究进展［J］.中国民族医药杂志,2010(6):57-59.

5.王丽君,姜虹.龙牙楤木多糖抗肿瘤活性及对荷瘤小鼠免疫功能的影响［J］.中国免疫学杂志,2011,27:130-134.

6.彭会军,邹群.植物多糖生物活性研究进展［J］.西北药学杂志,2008,23(6):406-408.

7.Zhao Ting,Mao Guanghua,Mao Riwen.Antitumor and immunomodulatory activity of a water-soluble low molecular weight polysaccharide from Schisandra chinensis(Turcz.) Baill［J］.Food and Chemical Toxicology,2013,55:609-616.

8.Zhang Ting,Gao Jie,Jin Zhengyu.Protective effects of polysaccharides from Lilium lancifolium on streptozotocin-induced diabetic mice［J］.International Journal of Biological Macromolecules,2014,65:436-440.

9.Liu Jun,Wen Xiaoyuan,Zhang Xueqing.Extraction,characterization and in vitro antioxidant activity of polysaccharides from black soybean［J］.International Journal of Biological Macromolecules,2015,72:1182-1190.

10.Bing S J,Kim M J,Ahn G,et al.Acidic polysaccharide of Panax ginseng regulates the mitochondria/caspase-dependent apoptotic pathway in radiation-induced damage to the jejunum in mice［J］.Acta Histochemica,2014,116(3):514-521.

11.雒羽.飞天蜈蚣七种有效成分的提取纯化工艺及其应用研究［D］.西安:西北大学,2012.

12.宁德利,阮洪生,韩承伟,等.紫外分光光度法测定长白中国楤木根、茎、叶中多糖含量［J］.黑龙江八一农垦大学学报,2009,21(3):67-69.

13.刘艳,田吉,何兵,等.四川楤木根茎叶嫩芽中多糖含量的紫外分光光度法测定［J］.时珍国医国药,2011,22(7):1653-1654.

14.蔡静.飞天蜈蚣七多糖提取纯化工艺、结构及抗氧化活性研究［D］.西安:西北大学,2011.

15.栗舒雅,李峰,张沙艳,等.长白楤木根多糖理化性质的研究［J］.安徽农业科学,2007,35(11):3214-3215.

16.王悦,陈阿梅,李秉超.长白楤木根茎叶水溶性多糖的纯化及组成分析［J］.氨基酸和生物源,2007,29(2):15-17.

17.Zhang Jing,Wang Haiyan,Xue Yusheng,et al.Cardioprotective and antioxidant activities of a polysaccharide from the root bark of Aralia elata(Miq.) Seem［J］.Carbohydre Polym,2013,93(2):442-448.

18.包淑云,赵志刚,陈靠山,等.超声波提取棉花根多糖的工艺研究［J］.时珍国医国药,2013,24(1):118-119.

19.刘延吉,李峰,郭迎春,等.苯酚—硫酸法测长白楤木根中多糖的含量［J］.安徽农业科学,2006,34(5):830-856.

20.尹龙萍,邓毅,贾伟,等.山豆根粗多糖最优化提取工艺研究［J］.中药材,2007,30(3):351-353.

21.Zhao Baotang,Zhang Ji,Guo Xiao,et al.Microwave-assisted extraction,chemical characterization of polysaccharides from Lilium davidii var.unicolor Salisb and its antioxidant activities evaluation［J］.Food Hydrocolloids,2013,31:346-356.

22.宋珅,赵保堂,殷振雄,等.籽瓜多糖的提取分离及单糖组成的GC-MS分析［J］.食品发酵与工业,2013,39(5):238-245.

23.Wang Junlong,Zhang Ji,Wang Xiaofang,et al.A comparison study on microwave-assisted extraction of Artemisia sphaerocephala polysaccharides with conventional method:molecule structure and antioxidant activities evaluation［J］.Int J Biol Marchomol,2009,45:483-492.

24.乔德亮,孙传伯,何晓梅,等.三角帆蚌多糖的微波辅助提取及脱蛋白工艺［J］.天然产物研究与开发,2014,26:902-908,925.

[1]	MENG Yao, YU Lian, QIN Xiang-Yu, LIU Chang, WANG Ze-Lin, ZHAO Chun-Jian, FU Yu-Jie. Optimization of the ActiveCompoundsExtraction Process from Fig Leaves by Anionic and Cationic Surfactants and Preliminary Functional Evaluation [J]. Bulletin of Botanical Research, 2020, 40(5): 789-794.
[2]	XING Wen-Miao, CHEN Min, FENG Chang-Yin, MENG Xian-Ming, TIAN Meng-Fei, MU Fan-Song, LUO Meng. Optimization of Ultrasound Assisted Acid Hydrolysis Process for Extracting Vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. Leaf [J]. Bulletin of Botanical Research, 2020, 40(1): 148-152.
[3]	WANG Xi-Gang, ZHAN Ya-Guang, ZHANG Gui-Qin, YAN Chao-Fu, LI Xiang, ZENG Fan-Suo. Influences of Fertilization on Growth and Fruiting of Fraxinus mandschurica [J]. Bulletin of Botanical Research, 2017, 37(2): 298-303.
[4]	WANG Hua1,2；ZHOU Li-Ping1,2；LI Meng-Sha1；ZHU Liang-Yu1,2；MU Li-Qiang2；NI Hong-Wei1. Optimization of Microwave-assisted Extraction of Syringin and Oleuropein from Syringa reticulata* [J]. Bulletin of Botanical Research, 2016, 36(1): 141-145.
[5]	LI Wen-Gang；SUN Xiao-Li；GE Yun-Long；ZHAO Xiu-Hua；ZU Yuan-Gang*. Preparation Optimization and Physical-chemical Properties of Lignin Diesel [J]. Bulletin of Botanical Research, 2015, 35(5): 795-800.
[6]	LU Qi；GAO Yue；XIANG Feng-Ying；ZU Yuan-Gang*；ZHANG Ying. Polysaccharide Extraction from Peony Seed Pods by Water Bath [J]. Bulletin of Botanical Research, 2015, 35(1): 154-157.
[7]	ZHAO Lian-Fei;CHEN Feng-Li;SUN Xi-Xiao;DU Xin-Qi;ZHAO Chun-Jian*;YAO Huan-Huan;YANG Lei;WANG Gui-Bin;ZU Yuan-Gang. Microwave-assisted Extraction of Paeoniflorin and Paeonol from Tree Peony Seed Coat and Content Determination [J]. Bulletin of Botanical Research, 2014, 34(4): 561-566.
[8]	MA Fei-Yue;DUAN Kai-Fang;WANG Xi-Qing;QIN Ming-Cong;LI Ji;FU Yu-Jie. Ionic Liquids-based Microwave-assisted Extraction of Biochanin A and Genistein from Dalbergia odorifera* T.Chen Leaves [J]. Bulletin of Botanical Research, 2013, 33(4): 494-498.
[9]	PENG Xiao;DUAN Ming-Hui;PAN You-Zhi;ZHANG Dong-Yang;DUAN Xi-Hua;. Microwave-assisted Extraction of Genistein from Pigeon Pea[Cajanus cajan*(L.) Millsp.] Roots [J]. Bulletin of Botanical Research, 2012, 32(6): 756-759.
[10]	ZHANG Yu;JIANG Shou-Gang;ZU Yuan-Gang;WANG Zhuo;FU Yu-Jie. Optimization of Extration of 7-xylosyl-10-deacetylpaclitaxel from Taxus cuspidata* [J]. Bulletin of Botanical Research, 2010, 30(3): 369-372.
[11]	MA Ying;LI Kai-Long;TIAN Xin-Hua;LI Jing. Culture in Vitro and Rapid Propagation System with Potentilla fruiticosa ‘Gold Drop’ [J]. Bulletin of Botanical Research, 2009, 29(5): 623-627.
[12]	NIE Jiang-Li, PEI Yi, ZU Yuan-Gang. Study on extractive process of fruits from Schisandra chinensis by supercritical carbon dioxide [J]. Bulletin of Botanical Research, 2005, 25(2): 213-215.