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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (3): 363-369.doi: 10.7525/j.issn.1673-5102.2015.03.007

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Isolation and Functional Analysis of FLC Gene from Poplar (Populus simonii×Populus nigra)

SUN Qi;QU Chun-Pu;ZHENG Mei-Zhu;LIU Guan-Jun*   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Online:2015-05-20 Published:2015-06-24
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Abstract: MADS-box family protein is an important class of transcription factors regulating flowering. FLC is a member of MADS-box transcription factors, and the FLC gene is controlled by many pathways, and then to regulate the flowering. We cloned a FLC-homolog gene in male flower buds from Populus simonii×Populus nigra. The open reading frame (ORF) of this gene had 726 bp which encoded a protein containing 241 amino acid. The molecular weight of the predicted protein was 27.559 KD, and the pI was 9.37. By real-time quantitative PCR, vernalization could down-regulate the expression of PnFLC 57.9%-84% in the root, stem and leaves tissues of poplar. By GUS staining, the promoter of the PnFLC is highly expressed in actively dividing tissues. Furthermore, by transforming Arabopsis, PnFLC down-regulated the expression of AtAP1, AtSOC1 and AtFT, while delayed the flowering in Arabidopsis.

Key words: Populus simonii×, Populus nigra, FLC gene, flowering, vernalization

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