Effects of Successive Subculture on the Field Colonization Adaptability of Brachythecium plumosum in the Qinling Mountains

doi:10.7525/j.issn.1673-5102.2026.05.016

Abstract

Abstract:

To investigate the effects of long-term artificial subculture on the field colonization adaptability of bryophyte crust seed sources， this study conducted indoor successive subculture， outdoor colonization， and artificial rainfall simulation scouring experiments on the saxicolous moss Brachythecium plumosum from the Qinling Mountains. The differences in field colonization adaptability among seed sources with different successive subculture generations were analyzed by measuring relevant growth and physiological indicators. The results showed that：（1）The initial generation（S0）， the first successive subculture generation（S1）， and the second successive subculture generation（S2） all colonized rapidly， with coverages exceeding 90% after 32 d， among which S0 exhibited the best overall growth performance. （2）After rainfall scouring， S0 showed the smallest reduction in coverage， and possessed the strongest crust stability and resistance to water erosion. （3） After 32 d of colonization， the photosynthetic pigment density of S0 remained stable， while the malondialdehyde（MDA） and soluble substance contents gradually decreased； S0 alleviated oxidative damage by increasing peroxidase（POD） activity and maintaining catalase（CAT） activity. In contrast， the photosynthetic pigment contents of S1 and S2 decreased significantly， while their MDA and soluble substance contents increased markedly. The antioxidant enzyme-responsive patterns of S1 were similar to that of S0， whereas S2 exhibited a distinct pattern， characterized primarily by a significant increase in CAT activity， with a limited increase in POD activity. （4）Redundancy analysis（RDA） showed that the physiological response patterns of S2 were significantly different from those of S0 and S1， with photosynthetic pigment content and antioxidant enzyme activities being the key indicators driving this differentiation. In conclusion， successive subculture weakens the adaptability of B. plumosum seed sources to natural environments. In engineering applications， the number of subculture generations should be reasonably controlled to ensure the ecological function and field colonization adaptability of bryophyte crust seed sources.

Key words: successive subculture, bryophyte biocrusts, Brachythecium plumosum, physiological characteristics, environmental adaptation

CLC Number:

Q945.79

Yuhan YAN, Qiuxia MO, Chongfeng BU, Yakun TANG. Effects of Successive Subculture on the Field Colonization Adaptability of Brachythecium plumosum in the Qinling Mountains[J]. Bulletin of Botanical Research, 2026, 46(5): 960-970.

Figures/Tables 8

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因素 Factor	株密度 Plant density		株高度 Plant height		盖度 Cover
因素 Factor	F	P	F	P	F	P
继代次数 Subculture time	3.208	0.043	8.345	<0.001	1.735	0.188
定植时间 Colonization time	23.875	<0.001	1.125	0.357	15.612	<0.001
继代次数×定植时间 Subculture time×Colonization time	0.871	0.548	5.357	<0.001	0.916	0.512

生理指标 Physiological indices	继代次数 Subculture time		定植时间 Colonization duration		继代次数×定植时间 Subculture time×Colonization duration
生理指标 Physiological indices	F	P	F	P	F	P
Chl a	2.901	0.094	38.145	<0.001	11.657	0.002
Chl b	3.769	0.054	38.035	<0.001	6.832	0.010
Car	2.322	0.140	38.481	<0.001	22.266	<0.001
MDA	1.825	0.203	6.860	0.022	4.818	0.029
SS	5.069	0.025	0.009	0.926	34.913	<0.001
SP	3.630	0.059	78.463	<0.001	31.305	<0.001
POD	0.864	0.446	38.312	<0.001	8.542	0.005
SOD	5.543	0.020	38.823	<0.001	3.142	0.080
CAT	3.113	0.081	9.210	0.010	3.790	0.053