Metabolic Differences Between Wild and Cultivated Species of Taxus cuspidata Based on GC-MS

doi:10.7525/j.issn.1673-5102.2021.05.018

Abstract

Abstract:

In order to explore the differences of metabolites between wild and cultivated Taxus cuspidata， nontargeted metabolomics was analyzed. Using GC-MS and multivariate statistical analysis to compare the bark of wild species and cultivated species， the differences in their metabolites were found. A total of 45 significant metabolites were detected， including 4 amino acids， 10 organic acids and some sugar alcohols. Among them， 22 metabolites were up-regulated and the 23 metabolites were down-regulated. KEGG pathway analysis showed that seven pathways had significant effects， including sulfur metabolism， arginine and proline metabolism， pentose phosphate pathway， unsaturated fatty acid biosynthesis， galactose metabolism， alanine， aspartate and glutamic acid metabolism， phenylpropanoid biosynthesis. The metabolites such as amino acids and organic acids were highly expressed in cultivated T. cuspidata. The stress resistance of wild T. cuspidata was weaker than that of cultivated T. cuspidata. However， the expression of carbohydrate was higher in wild species. T. cuspidata wild species had better glucose metabolism ability to resist low temperature stress. The results provided a theoretical basis for the protection of T. cuspidata wild species and stress resistance mechanism of T. cuspidata.

Key words: Taxus cuspidata, metabonomics, GC-MS

CLC Number:

S791.49

Long-Jie LIU, Ke-Xin WU, Yun-Fei DIAO, Zhong-Yue LI, Li-Qiang MU. Metabolic Differences Between Wild and Cultivated Species of Taxus cuspidata Based on GC-MS[J]. Bulletin of Botanical Research, 2021, 41(5): 798-806.

Figures/Tables 5

Fig.1

Fig.2

Fig.3

Table 1

Screening of differential metabolites

ID	名称Name	VIP	P-value	Fold change	log2FC
658	棕榈酸 palmitic acid	1.472 112 499	0.042 524 506	0.535 815 892	-0.900 190 724	下调
411	4-羟基苯甲醛 4-hydroxybenzaldehyde 2	1.544 527 986	0.003 955 057	1.941 45E-06	-18.974 436 85	下调
694	硬脂酸 stearic acid	1.497 638 559	0.028 629 822	0.535 337 429	-0.901 479 571	下调
506	4-羟基苯甲腈 4-Hydroxymandelonitrile	1.214 786 018	0.002 571 763	0.058 783 87	-4.088 435 851	下调
475	4-羟基苯甲酸 4-Hydroxybenzoic acid	1.368 793 998	0.021 544 072	0.669 615 096	-0.578 596 043	下调
155	4-羟基吡啶 4-HYDROXYPYRIDINE	1.443 822 285	0.009 498 300	0.840 256 426	-0.251 098 425	下调
789	龙胆二糖 Gentiobiose	1.503 333 156	0.002 166 261	3.170 004 126	1.664 484 718	上调
514	D-阿拉伯糖醇 D-Arabitol	1.465 817 496	0.000 396 016	7.113 321 81	2.830 523 433	上调
861	1-科斯糖 1-Kestose	1.268 962 621	0.001 518 882	193.666 081 7	7.597 427 495	上调
485	来苏糖 Lyxose	1.544 552 429	9.528E-05	240 552.384 5	17.875 991 58	上调
795	表儿茶素 Epicatechin	1.355 757 434	0.005 109 837	3.399 529 096	1.765 334 918	上调
425	天冬氨酸 aspartic acid	1.544 617 254	0.025 415 003	30 842.034 24	14.912 610 3	上调
571	3，4-二羟基苯甲酸 3，4-dihydroxybenzoic acid	1.380 354 325	0.017 728 938	1.839 624 364	0.879 411 21	上调
495	核糖 ribose	1.308 834 145	0.024 426 711	1.685 392 321	0.753 084 457	上调
173	磷酸甲酯 Methyl Phosphate	1.536 530 831	0.000 100 881	0.383 876 835	-1.381 284 591	下调
664	N-乙酰-β-D-甘露糖胺 N-Acetyl-beta-D-mannosamine	1.360 171 221	0.017 607 864	0.705 092 33	-0.504 115 907	下调
796	蜜二糖 melibiose	1.331 541 409	0.022 315 947	1.833 279 006	0.874 426 366	上调
824	半乳糖肌醇 Galactinol	1.380 398 803	0.005 019 350	192.384 961 2	7.587 852 217	上调
661	阿魏酸 ferulic acid	1.378 890 959	0.020 800 118	0.732 231 664	-0.449 627 934	下调
502	木糖醇 xylitol	1.125 511 036	0.020 225 209	5.213 271 765	2.382 189 069	上调
874	麦芽糖三醇 Maltotriitol	1.417 795 071	0.002 392 486	2.336 304 729	1.224 228 46	上调
811	表没食子儿茶素 Epigallocatechin	1.039 175 639	4.223 48E-06	22.554 946 83	4.495 371 98	上调
721	丁丙氧嘧啶 D-erythro-sphingosine	1.327 665 357	8.729 89E-08	198.005 361	7.629 395 681	上调
477	天冬酰胺 asparagine	1.271 606 986	0.044 071 336	0.786 124 917	-0.347 169 516	下调
163	硫酸 sulfuric acid	1.302 102 309	0.030 678 318	0.864 522 349	-0.210 024 835	下调
552	2-脱氧-D-半乳糖 2-Deoxy-D-galactose	1.544 150 397	0.008 833 495	2.629E-06	-18.537 053 48	下调
725	花生油酸 Arachidic acid	1.386 794 033	0.016 775 709	0.732 380 903	-0.449 333 921	下调
499	二氢香豆素 dihydrocoumarin 1	1.464 121 803	0.005 774 149	0.383 730 094	-1.381 836 182	下调
814	异麦芽酮糖醇 palatinitol	1.430 704 001	0.006 094 907	2.048 883 004	1.034 837 606	上调
538	瓜氨酸 citrulline	1.544 470 902	0.004 465 438	3.192 56E-07	-21.578 782 69	下调
426	鸟氨酸 ornithine	1.364 840 559	0.017 914 685	0.731 521 11	-0.451 028 597	下调
601	山梨糖 sorbose	1.544 410 152	0.001 487 191	805 180.731 4	19.618 953 12	上调
858	4-胆固醇-3-酮 4-Cholesten-3-one	1.544 608 860	0.000 762 440	59 354.002 67	15.857 057 7	上调
328	2，3-二甲基丁二酸 2，3-Dimethylsuccinic acid	1.444 865 796	0.008 480 610	0.899 640 039	-0.152 580 225	下调
376	2，4-二氨基丁酸 2，4-diaminobutyric acid	1.261 381 228	0.032 168 903	0.711 556 396	-0.490 949 99	下调
867	α-蜕皮激素 alpha-Ecdysone	1.544 592 096	0.016 276 997	77 151.015 39	16.235 397 52	上调
138	2-酮戊酸 2-Ketovaleric acid	1.343 071 542	0.021 088 473	0.795 735 998	-0.329 638 229	下调
309	尿嘧啶 uracil	1.323 357 352	0.023 793 329	0.749 594 248	-0.415 818 212	下调
61	2-羟基吡啶 2-hydroxypyridine	1.468 374 875	0.004 202 588	0.822 850 854	-0.281 297 136	下调
263	环己基甲酰胺 N-cyclohexylformamide	1.360 027 301	0.029 399 313	0.796 604 153	-0.328 065 094	下调
351	O-乙酰丝氨酸 O-acetylserine	1.336 593 662	0.046 415 957	1.736 223 273	0.795 952 486	上调
406	双（2-羟丙基）氨基 Bis（2-hydroxypropyl）amine	1.336 869 479	0.032 568 816	1.241 578 555	0.312 175 544	上调
669	顺式-植醇 cis-Phytol	1.513 264 413	0.000 672 088	0.578 733 293	-0.789 029 454	下调
456	3-苯基乳酸 3-Phenyllactic acid	1.216 295 145	0.043 426 151	15.889 975 46	3.990 044 992	上调
501	茉莉酸甲酯 Methyl jasmonate	1.397 339 118	0.006 402 332	0.708 984 225	-0.496 174 568	下调

Table 1

Fig.4

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