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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 798-806.doi: 10.7525/j.issn.1673-5102.2021.05.018

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Metabolic Differences Between Wild and Cultivated Species of Taxus cuspidata Based on GC-MS

Long-Jie LIU1, Ke-Xin WU1, Yun-Fei DIAO2, Zhong-Yue LI1, Li-Qiang MU1()   

  1. 1.Forestry College,Northeast Forestry University,Harbin 150040
    2.Mudingjiang Forest Ecosystem Research Station,Harbin 150081
  • Received:2021-03-20 Online:2021-09-20 Published:2021-07-05
  • Contact: Li-Qiang MU E-mail:mlq0417@163.com
  • About author:LIU Long-Jie(1993—),male,master graduate,mainly engaged in the protection and utilization of resource plants.
  • Supported by:
    Science and Technology Basic Resources Investigation Program of China(2019FY100500);Regeneration Diffusion Mechanism and Conservation Strategy of Taxus cuspidata wild population(20189037)


In order to explore the differences of metabolites between wild and cultivated Taxus cuspidata, nontargeted metabolomics was analyzed. Using GC-MS and multivariate statistical analysis to compare the bark of wild species and cultivated species, the differences in their metabolites were found. A total of 45 significant metabolites were detected, including 4 amino acids, 10 organic acids and some sugar alcohols. Among them, 22 metabolites were up-regulated and the 23 metabolites were down-regulated. KEGG pathway analysis showed that seven pathways had significant effects, including sulfur metabolism, arginine and proline metabolism, pentose phosphate pathway, unsaturated fatty acid biosynthesis, galactose metabolism, alanine, aspartate and glutamic acid metabolism, phenylpropanoid biosynthesis. The metabolites such as amino acids and organic acids were highly expressed in cultivated T. cuspidata. The stress resistance of wild T. cuspidata was weaker than that of cultivated T. cuspidata. However, the expression of carbohydrate was higher in wild species. T. cuspidata wild species had better glucose metabolism ability to resist low temperature stress. The results provided a theoretical basis for the protection of T. cuspidata wild species and stress resistance mechanism of T. cuspidata.

Key words: Taxus cuspidata, metabonomics, GC-MS

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