Metabolomics Analysis of Lilium davidii var. unicolor in Response to Autotoxicity Stress

doi:10.7525/j.issn.1673-5102.2024.05.016

Abstract

Abstract:

In order to explore the metabolic mechanism of Lanzhou lily（Lilium davidii var. unicolor） in response to autotoxicity stress， one-year-old（Lily1）， two-year-old（Lily2） and three-year-old（Lily3） Lanzhou lilies were used as materials， and gas chromatography-mass spectrometry（GC-MS） was used to analyze the metabolomic changes of Lanzhou lily under continuous cropping years， and a total of 124 metabolites were detected and identified. R software was used to normalize the samples， and orthogonal partial least squares discriminant analysis（OPLS-DA） was used to find differential metabolites， and related metabolite pathways were screened for joint analysis. The results showed that there were 16 differential metabolites（lipids， flavonoids， saccharides and phenols） between Lily1 and Lily2， and all of them were up-regulated； and it was significantly enriched in tyrosine metabolism， propionic acid metabolism and flavonoid biosynthetic metabolic pathways. The contents of lipids， flavonoids and saccharides increased， indicating that in the second year of continuous cropping， Lanzhou lily responded to autotoxicity stress by actively synthesizing lipids， flavonoids and saccharides. There were 21 differential metabolites between the metabolomes of Lily1 and Lily3， including one up-regulated metabolite（3-α-mannobiose） and 20 down-regulated metabolite packages （amines， acids， and lipids）； and five metabolic pathways were significantly enriched， including biosynthesis of tropane， piperidine and pyridine alkaloids， phosphate and hypophosphite metabolism， lysine degradation， glutathione metabolism and D-amino acid metabolism. There were 21 differential metabolites between Lily2 and Lily3 groups， and the only up-regulated metabolite was 3-α-mannobiose. The results showed that in the third year of continuous cropping of Lanzhou lily， saccharides， as carbohydrates， were directly involved in cell membrane stability， actively responded to autotoxicity stress. The 20 down-regulated metabolites were amines， acids and lipids； only the propionic acid metabolism and flavonoid biosynthetic metabolism pathways were significantly enriched.

Key words: Lilium davidii var. unicolor, metabolomics, autotoxicity, GC-MS

CLC Number:

Q945

Zhiyuan CHENG, Yanjiang ZHANG, Yue SHEN, Guang LI, Qin CHEN, Jie LI, Yanhong XING. Metabolomics Analysis of Lilium davidii var. unicolor in Response to Autotoxicity Stress[J]. Bulletin of Botanical Research, 2024, 44(5): 793-804.

Figures/Tables 9

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分组 Group	OPLS-DA模型评价参数 OPLS-DA model evaluation parameters			OPLS-DA验证模型参数 OPLS-DA validation model parameters
分组 Group	R²X	R²Y	Q²	R²X	R²Y	Q²
L1	0.159	0.898	0.523	0.307	0.973	0.684
L2	0.230	0.645	0.439	0.720	0.998	0.705
L3	0.222	0.668	0.503	0.704	0.999	0.747

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