Genetic Diversity of Plant Tetraenamongolica by ISSR Markers

doi:10.7525/j.issn.1673-5102.2021.03.018

Abstract

Abstract:

Tetraena mongolica is an endangered plant belonging to a single genus， known as the ‘plant giant panda’. It has been included in the first batch of national rare and endangered plant red papers. It is a special shrub endemic to the west of Ordos Plateau and its distributed area is small. Six different geographical populations of T.mongolica were used as test materials. Six of the 30 UBC primers were screened for the most suitable primers， and the ISSR amplification reaction system and amplification procedure were reasonably optimized. The 370 bands were amplified from the primers， and 71% of T.mongolica was polymorphic bands. The results of the analysis can be shown as follows： ①The Nei’s gene diversity index was 0.316 8 and Shannon’s polymorphism information index was 0.458 6. The genetic diversity levels of T.mongolica different provenances were little different； ②The percentage of groups polymorphism allele in the population ranged from 70.00%-83.33%， the Nei’s genetic diversity index was 0.286 5-0.350 8， and the Shannon’s polymorphism information index was 0.423 6-0.504 9. The G_st of genetic differentiation coefficient between the six populations was 0.125 3， the results explain that there is a certain degree of genetic differentiation among populations； ③The gene flow of the six populations was 3.491 5>1， indicating that there was a certain gene flow among the six populations， which could prevent the genetic differentiation caused by genetic drift； ④Cluster analysis was used to classify the tetrahedrons of six different geographic populations into three groups. The populations of Qianligou， Yikebulage， and Balagong clustered into the first category， and then they were clustered with the Dengkou-taositu rabbits into the second category. There are two major categories， and the population of Natural Reserve of T.mongolica and the population Gandle Mountain constituted the third largest category. This shows that distance factors are the main factors that affect genetic differentiation among T.mongolica populations， but different habits conditions also have an impact on the genetic differentiation of T.mongolica populations.

Key words: Tetraenamongolica, genetic diversity, ISSR, population

CLC Number:

Q949.752.6

Yi-Zhong DUAN, Hai-Tao WANG, Ge-Ge ZHANG, Wei-Na YAN. Genetic Diversity of Plant Tetraenamongolica by ISSR Markers[J]. Bulletin of Botanical Research, 2021, 41(3): 466-473.

Figures/Tables 7

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编号 Note	地理位置 Location	纬度 Latitude (N)	经度 Longitude (E)	采样数 Hits	编号 Number
1	千里沟 Qianligou	106°50′05″	39°50′08″	11	QLG(1-11)
2	伊克布拉格 Yikebulage	106°48′56″	40°04′94″	11	YKBLG(1-11)
3	甘德尔山 Gandeershan	106°46′20″	39°32′18″	11	GDES(1-11)
4	四合木自然保护区 Sihemu nature reserve	106°46′03″	39°25′60″	11	SHMZRBHQ(1-11)
5	磴口-桃司兔 Dengkou-Taositu	107°05′23″	40°15′23″	11	DK-TST(1-11)
6	巴拉贡 Balagong	107°08′54″	40°15′32″	11	BLG(1-11)

序号 Note	引物 Primer	退火温度 Annealing temperature (℃)	序列 Sequence
1	810	45	GAGAGAGAGAGAGAGAT
2	811	45	GAGAGAGAGAGAGAGAC
3	814	48	CTCTCTCTCTCTCTCTA
4	824	45	TCTCTCTCTCTCTCTCG
5	835	42	AGAGAGAGAGAGAGAGYC
6	873	45	GACAGACAGACAGACA

引物序号 Primerse quence number	引物序列 Primer sequence	总条带数 The total number of bands	多态性条带数 Polymorphic band number	多态性百分率 Polymorphism percentage (%)
UBC810	GAGAGAGAGAGAGAGAT	66	55	84
UBC811	GAGAGAGAGAGAGAGAC	64	51	80
UBC814	CTCTCTCTCTCTCTCTA	56	35	63
UBC824	TCTCTCTCTCTCTCTCG	60	31	51
UBC835	AGAGAGAGAGAGAGAGYC	66	56	85
UBC873	GACAGACAGACAGACA	58	33	57
总计Total		370	261	71

种群 Population	多态位点数 Number of polymorphicloci	多态位点比率 Polymorphic loci percentage(%)	等位基因观察值 Observation of allele	有效等位基因 Effective alleles	Nei’s多样性指数 Nei's genetic diversity	Shannon’s信息指数 Shannon’s index
千里沟Qianligou	21	70.00	1.700 0	1.546 1	0.295 9	0.425 6
伊克布拉格Yikebulage	23	76.67	1.766 7	1.608 2	0.330 7	0.474 8
甘德尔山Gandeershan	23	76.67	1.766 7	1.555 1	0.309 5	0.450 7
四合木自然保护区 Sihemu nature reserve	25	83.33	1.833 3	1.642 1	0.350 8	0.504 9
桃司兔Taositu	23	76.67	1.766 7	1.498 9	0.286 5	0.423 6
巴拉贡Balagong	23	76.67	1.766 7	1.590 5	0.327 1	0.471 8
平均值Average	23	76.67	1.766 7	1.573 5	0.316 8	0.458 6

种群 Populations	千里沟 Qianligou	伊克布拉格 Yikebulage	甘德尔山 Gandeershan	四合木自然保护区 Sihemu nature reserve	桃司兔 Taositu	巴拉贡 Balagong
QLG	—	0.952 6	0.974 4	0.906 9	0.943 6	0.953 4
YKBLG	0.048 6	—	0.932 6	0.926 1	0.920 6	0.940 2
GDES	0.061 7	0.069 8	—	0.943 5	0.908 4	0.952 0
SHMZRBHQ	0.097 8	0.076 8	0.058 2	—	0.949 6	0.956 9
DK-TST	0.058 1	0.082 7	0.096 1	0.051 8	—	0.961 9
BLG	0.047 8	0.025 9	0.049 2	0.044 0	0.038 8	—