Response of Photosynthesis and Leaf Morphological Characteristics to Drought Stress in Glycyrrhiza uralensis

doi:10.7525/j.issn.1673-5102.2021.03.016

Abstract

Abstract:

The changes of photosynthesis， stomatal changes and glandular morphologic of leaves in Glycyrrhiza uralensis were measured with pot-culture drought stress in greenhouse， and the drought resistance of them were evaluated by membership function and drought resistance coefficient. The response of leaf photosynthesis， stomatal micromorphology and glandular morphology to drought stress was studied by pot controlled water natural drought method. The results showed that leaf net photosynthetic rate（P_n）， stomatal conductance（G_s） and transpiration rate（T_r） have been rising since the degree of drought resistance after the trend of lower， and the intercellular CO₂（C_i） concentration increased rapidly in the severe drought stress. Moreover， leaf total stomata density and stomata opening than with the intensification of the degree of drought stress， the trend of first increases and then decreases， and stomatal opening width is gradually decreasing trend. Importantly， since the degree of drought stress， leaf epidermis and lower epidermis gland density overall showed a trend of increase in the total number of gland color as the degree of drought stress increased gradually deepened， shape appears irregular fold and retraction. The glandular characteristics on the leaf surface of G. uralensis are involved in the regulation of drought resistance， so as to avoid the damage of drought stress to G.uralensis. Under severe drought stress， stress accelerated the programmed death of stomatal cells（PCD）， and G.uralensis seedlings lost their ability to resist drought.

Key words: Glycyrrhiza uralensis, drought stress, photosynthesis, stomatal, glands

CLC Number:

S567.7

Dong ZHANG, Yan LIU, Han ZHANG, Zi-Jian ZHANG, Yang WANG, Mei-Cen LIU. Response of Photosynthesis and Leaf Morphological Characteristics to Drought Stress in Glycyrrhiza uralensis[J]. Bulletin of Botanical Research, 2021, 41(3): 449-457.

Figures/Tables 6

Fig.1

PlateⅠ

Table1

Table 2

Table 3

Fig.2

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材料 Material	处理 Treatment	上表皮密度 Stomatal density of upper epidermis （个·mm^-2）	下表皮密度 Stomatal density of lower epidermis （个·mm^-2）	上表皮开张比 Porosity opening ratio of upper epidermis（%）	下表皮开张比 Porosity opening ratio of lower epidermis（%）	上表皮开张宽度 Stomatal opening width of upper epidermis（μm）	下表皮开张宽度 Stomatal opening width of lower epidermis（μm）
新叶 Young leaves	CK	75.3±4.5b	216.7±4.5ab	46.0±1.0b	34. 7±1.5b	1.36±0.05b	2.14±0.05a
	LS	91.3±5.1a	224±2.6a	53. 7±1.5a	48.0±2.0a	1.72±0.04a	1.74±0.05b
	MS	66.7±3.5bc	203.7±3.5bc	44.3±1.2b	30.7±2.1b	0.78±0.04c	0.60±0.03c
	SS	61.7±4.7c	180.7±3.1c	38.3±1.5c	13.7±1.5c	0.58±0.03d	0.52±0.01c
成熟叶 Mature leaves	CK	71.3±4.5b	260±3.0a	68. 7±1.5a	57.33±0.6b	1.92±0.04a	2.4±0.1a
	LS	104.7±6.5a	201.3±4.2b	71.3±1.5a	73.33±1.5a	1.89±0.03a	2.1±0.02b
	MS	87.7±2.5b	142.3±1.5c	30.7±2.5b	51±1.0b	1.15±0.05b	0.77±0.03c
	SS	38.3±3.5c	213.3±2.5b	22.3±2.5c	10.33±1.5c	0.95±0.05c	0.73±0.04c
衰老叶 Aging leaves	CK	76.3±2.5a	167.3±2.5a	58±2.0b	23.67±1.5b	1.18±0.03b	0.98±0.02a
	LS	62.7±2.5b	141.3±3.5b	67.7±1.2a	29±1.0a	1.38±0.03a	0.94±0.03a
	MS	46.3±3.5c	173.7±2.5a	21±2.6c	12.33±1.5cd	0.78±0.03c	0.71±0.02bc
	SS	24.0±3.6d	163.7±4.0a	17.7±0.6c	7.67±1.5d	0.77±0.03c	0.58±0.03c

材料 Material	处理 Treatment	上表皮 Upper epidermis （ind.·mm^-2）	下表皮 Lower epidermis （ind.·mm^-2）	表皮 Epidermis （ind.·mm^-2）
新叶 Young leaves	CK	2.18±0.15c	5.08±0.23c	7.26±0.12c
	LS	2.18±0.44c	7.63±0.15ab	9.81±0.58b
	MS	3.63±0.44b	6.17±0.15c	9.80±0.44b
	SS	5.16±0.38a	8.35±0.15a	13.51±0.52a
成熟叶 Mature leaves	CK	2.98±0.44a	4.79±0.58c	7.77±0.58c
	LS	3.50±0.15bc	5.48±0.44a	8.98±0.65bc
	MS	1.96±0.22b	5.81±0.36c	7.77±0.44c
	SS	1.16±0.30c	7.63±0.22b	8.79±0.22a
衰老叶 Aging leaves	CK	0.87±0.15bc	4.54±0.44b	5.41±0.15b
	LS	1.69±0.22b	3.60±0.30c	5.29±0.22b
	MS	1.23±0.15b	3.85±0.22b	5.08±0.29b
	SS	2.40±0.36a	6.90±0.44a	9.30±0.36a

材料 Material	处理 Treatment	上表皮 Upper epidermis （ind./mm²）	下表皮 Lower epidermis （ind./mm²）	表皮 Epidermis （ind./mm²）
新叶 Young leaves	CK	5.81±0.44bc	4.72±0.36c	10.53±0.73c
	LS	3.63±0.36c	3.63±0.22c	17.26±0.94b
	MS	11.98±0.58a	10.89±0.58a	22.88±0.91a
	SS	2.18±0.22c	9.08±0.73ab	11.26±0.58c
成熟叶 Mature leaves	CK	3.99±0.29b	5.08±0.51c	9.08±0.36b
	LS	1.09±0.15c	5.45±0.44c	6.54±0.29c
	MS	5.08±0.44b	22.50±0.87a	27.60±0.44a
	SS	9.80±0.80a	17.79±0.94b	27.60±0.80a
衰老叶 Aging leaves	CK	5.08±0.36b	5.08±0.51b	10.17±0.15b
	LS	9.80±0.22a	2.54±0.29c	12.35±0.22a
	MS	4.36±0.22b	5.45±0.36b	9.80±0.58b
	SS	1.45±0.15c	8.35±0.65a	9.80±0.44b

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