Leaf Phenotype Variation and Heterosis in F1 Progeny of Cross Between Castanea mollissima and C.henryi

doi:10.7525/j.issn.1673-5102.2021.02.016

Abstract

Abstract:

In order to explore the genetic variation and heterosis of leaf traits in cross F₁ progeny of Castanea， 14 traits of leaf phenotype and photosynthetic physiology of F₁ progeny（including 235 individuals） of 9 combinations （including interspecific and intraspecific cross between C.mollissima and C.henryi） and their parents were studied by using the statistical methods of Nested anova， Pearson correlation analysis and principal component analysis. The results showed： ①The average coefficient of variation of 14 traits ranged from 4.40%-27.76%， indicating that there were abundant genetic diversity in leaf phenotype and photosynthetic physiological traits in F₁ hybrid progeny of Castanea. ②The average of phenotypic differentiation coefficient among combination were 32.32%， which showed that the variation within combinations was the main source of variation. ③The mid-parent heterosis rate of 14 traits ranged from -27.16%-90.53%， and the heterosis of different combinations was different. ④The Pearson correlation analysis showed that there were 46 and 13 pairs of correlations reached extremely significant levels（P<0.01） and significant levels（P<0.05）， respectively， and 5 leaf phenotype traits， such as leaf length and leaf width， and 4 photosynthetic physiological indexes， such as chlorophyll a and chlorophyll b， had a great influence on each other. ⑤2 crossing combinations ‘Kuili’×YLZ No.15 and ‘Kuili’×YLZ No.1 were finally selected by principal component analysis， and their offspring had the characteristics of large leaf area， high dry matter and photosynthetic pigment content， which can provide the main materials for further screening of excellent offspring.

Key words: Castanea, hybrid offspring, genetic variation, heterosis

CLC Number:

S792.17

Ping-Sheng ZHANG, Xi-Bing JIANG, Bang-Chu GONG, Yang XU, Jun-sheng LAI, Cong-Lian WU. Leaf Phenotype Variation and Heterosis in F₁ Progeny of Cross Between Castanea mollissima and C.henryi[J]. Bulletin of Botanical Research, 2021, 41(2): 281-294.

Figures/Tables 7

Table 1

Table 2

Genetic variation analysis of leaf phenotype and Photosynthetic physiological characters in cross F1 generation of Castanea

杂交组合 Hybrid combinations	参数 Parameter	叶长 LL（cm）	叶宽 LW（cm）	叶形指数/LSI	叶面积 LA（cm²）	叶柄长 PL（mm）	叶鲜重 LFW（g）	叶干重 LDW（g）	含水率 LMC（%）	比叶重 SLW（g·cm^-2）	叶厚 LT（mm）	叶绿素a Chla（mg·g^-1）	叶绿素b Chlb（mg·g^-1）	叶绿素总量 Chla+b（mg·g^-1）	类胡萝卜素 Cx.c（mg·g^-1）
C1	平均值Mean	16.51BCD	5.98B	2.78B	74.42BC	15.35BC	1.51B	0.71C	52.88%B	0.0097A	0.142C	0.44AB	0.23BC	0.67ABC	0.52A
	CV(%)	8.12	7.69	10.79	12.05	25.47	21.85	16.90	5.73	8.25	6.34	13.64	13.04	13.43	23.08
	T_a(%)	103.9	81.81	127.52	102.72	91.31	110.22	120.34	93.35	118.29	85.54	95.65	92.00	95.71	106.12
C2	平均值Mean	17.80A	6.03B	2.99A	76.92B	16.30AB	1.57B	0.78B	50.38%C	0.0100A	0.144C	0.43AB	0.23BC	0.67ABC	0.51AB
	CV(%)	9.78	12.77	10.70	20.18	16.26	28.03	26.92	4.21	11.00	10.42	18.60	13.04	16.42	27.45
	T_a(%)	106.46	80.94	132.89	102.23	91.21	112.95	127.87	89.98	123.46	85.71	110.26	104.55	109.84	124.39
C3	平均值Mean	16.55BC	5.87B	2.85B	69.19CD	15.20BC	1.18CD	0.52F	56.26%A	0.0075C	0.206B	0.41BC	0.22CD	0.63CD	0.45B
	CV(%)	6.59	7.84	7.72	11.71	17.04	14.41	17.31	3.66	12.00	32.52	19.51	13.64	17.46	31.11
	T_a(%)	97.01	83.74	116.33	95.50	79.71	85.51	82.54	102.59	87.21	121.89	110.81	100.00	106.78	121.62
C4	平均值Mean	16.93B	5.54C	3.11A	64.75DE	15.29BC	1.15D	0.54EF	53.02%B	0.0084B	0.322A	0.39CD	0.21D	0.60DE	0.44B
	CV(%)	11.46	14.98	10.93	24.53	16.22	25.22	24.07	3.75	10.71	7.76	15.38	9.52	15.00	25.00
	T_a(%)	94.53	75.07	127.46	84.33	81.03	80.42	84.38	95.74	101.20	190.53	111.43	105.00	109.09	133.33
C5	平均值Mean	16.18BCD	5.36C	3.06A	62.13E	15.51BC	1.31CD	0.62D	52.45%B	0.0100A	0.190B	0.43AB	0.23BC	0.66ABC	0.47AB
	CV(%)	9.15	10.82	10.78	17.87	17.79	22.90	20.97	4.58	9.00	41.05	16.28	13.04	15.15	21.28
	T_a(%)	94.84	76.46	124.90	85.76	81.33	94.93	98.41	95.64	116.28	112.43	116.22	104.55	111.86	127.03
C6	平均值Mean	15.73D	5.29C	3.03A	59.45E	16.21AB	1.27CD	0.60DE	53.25%B	0.0100A	0.140C	0.36D	0.21D	0.57E	0.37C
	CV(%)	9.98	11.91	10.89	19.50	18.45	23.62	20.00	4.98	10.00	12.14	33.33	19.05	28.07	45.95
	T_a(%)	104.59	78.25	135.27	93.40	95.52	116.51	122.45	96.50	129.87	84.34	100.00	100.00	100.00	102.78
C7	平均值Mean	15.95CD	5.38C	3.00A	62.26E	14.31C	1.33C	0.62D	53.46%B	0.0099A	0.156C	0.41ABC	0.22CD	0.63BCD	0.46AB
	CV(%)	9.15	10.22	8.00	18.47	12.37	27.07	25.81	4.68	13.13	10.90	17.07	9.09	15.87	28.26
	T_a(%)	101.66	76.86	132.74	91.65	84.62	107.26	110.71	96.83	120.73	91.76	100.00	95.65	100.00	117.95
C8	平均值Mean	18.19A	6.49A	2.84B	85.45A	17.05A	1.77A	0.86A	51.06%C	0.0102A	0.153C	0.45A	0.24AB	0.70AB	0.52A
	CV(%)	7.09	10.17	9.15	15.81	16.72	19.21	18.60	4.11	9.80	13.73	15.56	12.50	12.86	21.15
	T_a(%)	101.45	72.84	137.86	83.82	111.88	89.85	87.76	98.84	112.09	81.82	145.16	126.32	140.00	185.71
C9	平均值Mean	18.02A	6.61A	2.75B	87.08A	15.88AB	1.78A	0.85AB	52.55%B	0.0098A	0.146C	0.45A	0.25A	0.71A	0.49AB
	CV(%)	7.44	7.87	6.55	13.19	16.62	16.29	16.47	3.86	12.24	13.70	20.00	16.00	17.14	26.53
	T_a(%)	105.50	77.40	132.85	89.20	102.92	93.19	88.54	102.82	104.26	78.07	132.35	119.05	131.48	153.13
总样本 Total	平均值Mean	16.87	5.84	2.93	71.29	15.68	1.43	0.68	52.81%	0.01	0.18	0.42	0.23	0.65	0.47
	CV(%)	8.75	10.47	9.50	17.03	17.44	22.07	20.78	4.40	10.68	16.51	18.82	13.21	16.82	27.76
	T_a(%)	101.10	78.15	129.76	92.07	91.06	98.98	102.56	96.92	112.60	103.57	113.54	105.24	111.64	130.23
组合间F值 F value among combination		13.11^**	20.88^**	7.53^**	23.27^**	2.91^*	19.05^**	26.98^**	18.79^**	28.17^**	71.77^**	4.69^**	6.50^**	5.33^**	4.87^**
组合内F值 F value within combination		9.65^**	18.59^**	7.82^**	15.54^**	3.91^**	12.57^**	10.95^**	6.80^**	4.69^**	34.89^**	14.71^**	12.78^**	14.87^**	10.68^**

Table 2

Table 3

Table 4

Heterosis analysis of leaf phenotype and photosynthetic physiological characters in cross F1 generation of Castanea

性状 Trait	杂交组合 Hybrid combinations	母本 Female	父本 Male	中亲值 MPV	中亲优势 H_m	中亲优势率 RH_m（%）	高亲值 BPV	超亲优势 H_b	超亲优势率 RH_b（%）
叶长 LL(cm)	C1	16.07	15.71	15.89	0.62	3.90	16.07	0.44	2.74
	C2	16.07	17.37	16.72	1.08^**	6.46	17.37	0.43	2.48
	C3	18.44	15.67	17.06	-0.51^*	-2.99	18.44	-1.89^**	-10.25
	C4	18.44	17.37	17.91	-0.98^**	-5.47	18.44	-1.51^**	-8.19
	C5	15.67	18.44	17.06	-0.88^**	-5.16	18.44	-2.26^**	-12.26
	C6	15.67	14.41	15.04	0.69	4.59	15.67	0.06	0.38
	C7	15.71	15.67	15.69	0.26	1.66	15.71	0.24	1.53
	C8	18.49	17.37	17.93	0.26	1.45	18.49	-0.30	-1.62
	C9	18.49	15.67	17.08	0.94^**	5.50	18.49	-0.47	-2.54
叶宽 LW(cm)	C1	7.38	7.23	7.31	-1.33^**	-18.19	7.38	-1.40^**	-18.97
	C2	7.38	7.51	7.45	-1.42^**	-19.06	7.51	-1.48^**	-19.71
	C3	7.25	6.77	7.01	-1.14^**	-16.26	7.25	-1.38^**	-19.03
	C4	7.25	7.51	7.38	-1.84^**	-24.93	7.51	-1.97^**	-26.23
	C5	6.77	7.25	7.01	-1.65^**	-23.54	7.25	-1.89^**	-26.07
	C6	6.77	6.75	6.76	-1.47^**	-21.75	6.77	-1.48^**	-21.86
	C7	7.23	6.77	7.00	-1.62^**	-23.14	7.23	-1.85^**	-25.59
	C8	10.30	7.51	8.91	-2.42^**	-27.16	10.30	-3.81^**	-36.99
	C9	10.30	6.77	8.54	-1.93^**	-22.60	10.30	-3.69^**	-35.83
叶厚 LT(mm)	C1	0.164	0.168	0.166	-0.024^**	-14.46	0.168	-0.026^**	-15.48
	C2	0.164	0.171	0.168	-0.024^**	-14.29	0.171	-0.027^**	-15.79
	C3	0.167	0.171	0.169	0.037^**	21.89	0.171	0.035^**	20.47
	C4	0.167	0.171	0.169	0.153^**	90.53	0.171	0.151^**	88.30
	C5	0.171	0.167	0.169	0.021	12.43	0.171	0.019	11.11
	C6	0.171	0.160	0.166	-0.026^**	-15.66	0.171	-0.031^**	-18.13
	C7	0.168	0.171	0.170	-0.014^**	-8.24	0.171	-0.015^**	-8.77
	C8	0.203	0.171	0.187	-0.034^**	-18.18	0.203	-0.05^**	-24.63
	C9	0.203	0.171	0.187	-0.041^**	-21.93	0.203	-0.057^**	-28.08
叶面积 LA(cm²)	C1	75.43	69.46	72.45	1.97	2.72	75.43	-1.01	-1.34
	C2	75.43	75.04	75.24	1.68	2.23	75.43	1.49	1.98
	C3	78.51	66.39	72.45	-3.26^*	-4.50	78.51	-9.32^**	-11.87
	C4	78.51	75.04	76.78	-12.03^**	-15.67	78.51	-13.76^**	-17.53
	C5	66.39	78.51	72.45	-10.32^**	-14.24	78.51	-16.38^**	-20.86
	C6	66.39	60.91	63.65	-4.20	-6.60	66.39	-6.94^*	-10.45
	C7	69.46	66.39	67.93	-5.67^*	-8.35	69.46	-7.20^**	-10.37
	C8	128.85	75.04	101.95	-16.50^**	-16.18	128.85	-43.4^**	-33.68
	C9	128.85	66.39	97.62	-10.54^**	-10.80	128.85	-41.77^**	-32.42
叶形指数 LSI	C1	2.18	2.17	2.18	0.60^**	27.52	2.18	0.60^**	27.52
	C2	2.18	2.32	2.25	0.74^**	32.89	2.32	0.67^**	28.88
	C3	2.55	2.34	2.45	0.40^**	16.33	2.55	0.30^**	11.76
	C4	2.55	2.32	2.44	0.67^**	27.46	2.55	0.56^**	21.96
	C5	2.34	2.55	2.45	0.61^**	24.90	2.55	0.51^**	20.00
	C6	2.34	2.14	2.24	0.79^**	35.27	2.34	0.69^**	29.49
	C7	2.17	2.34	2.26	0.74^**	32.74	2.34	0.66^**	28.21
	C8	1.80	2.32	2.06	0.78^**	37.86	2.32	0.52^**	22.41
	C9	1.80	2.34	2.07	0.68^**	32.85	2.34	0.41^**	17.52
叶柄长 PL(mm)	C1	17.96	15.65	16.81	-1.46	-8.69	17.96	-2.61^*	-14.53
	C2	17.96	17.77	17.87	-1.57^**	-8.79	17.96	-1.66^**	-9.24
	C3	19.97	18.16	19.07	-3.87^**	-20.29	19.97	-4.77^**	-23.89
	C4	19.97	17.77	18.87	-3.58^**	-18.97	19.97	-4.68^**	-23.44
	C5	18.16	19.97	19.07	-3.56^**	-18.67	19.97	-4.46^**	-22.33
	C6	18.16	15.78	16.97	-0.76	-4.48	18.16	-1.95^*	-10.74
	C7	15.65	18.16	16.91	-2.60^**	-15.38	18.16	-3.85^**	-21.20
	C8	12.70	17.77	15.24	1.81^**	11.88	17.77	-0.72	-4.05
	C9	12.70	18.16	15.43	0.45	2.92	18.16	-2.28^**	-12.56
叶鲜重 LFW(g)	C1	1.47	1.27	1.37	0.14	10.22	1.47	0.04	2.72
	C2	1.47	1.31	1.39	0.18^*	12.95	1.47	0.10	6.80
	C3	1.55	1.20	1.38	-0.20^**	-14.49	1.55	-0.37^**	-23.87
	C4	1.55	1.31	1.43	-0.28^**	-19.58	1.55	-0.40^**	-25.81
	C5	1.20	1.55	1.38	-0.07	-5.07	1.55	-0.24^**	-15.48
	C6	1.20	0.97	1.09	0.18^*	16.51	1.20	0.07	5.83
	C7	1.27	1.20	1.24	0.09	7.26	1.27	0.06	4.72
	C8	2.62	1.31	1.97	-0.20^**	-10.15	2.62	-0.85^**	-32.44
	C9	2.62	1.20	1.91	-0.13^*	-6.81	2.62	-0.84^**	-32.06
叶干重 LDW(g)	C1	0.63	0.55	0.59	0.12^**	20.34	0.63	0.08^*	12.70
	C2	0.63	0.59	0.61	0.17^**	27.87	0.63	0.15^**	23.81
	C3	0.69	0.56	0.63	-0.11^**	-17.46	0.69	-0.17^**	-24.64
	C4	0.69	0.59	0.64	-0.10^**	-15.63	0.69	-0.15^**	-21.74
	C5	0.56	0.69	0.63	-0.01	-1.59	0.69	-0.07^**	-10.14
	C6	0.56	0.42	0.49	0.11^**	22.45	0.56	0.04	7.14
	C7	0.55	0.56	0.56	0.06	10.71	0.56	0.06	10.71
	C8	1.36	0.59	0.98	-0.12^**	-12.24	1.36	-0.50^**	-36.76
	C9	1.36	0.56	0.96	-0.11^**	-11.46	1.36	-0.51^**	-37.50
叶片含水率 LMC（%）	C1	56.89	56.41	56.65	-3.77^**	-6.65	56.89	-4.01^**	-7.05
	C2	56.89	55.09	55.99	-5.61^**	-10.02	56.89	-6.51^**	-11.44
	C3	55.67	54.00	54.84	1.42^**	2.59	55.67	0.59	1.06
	C4	55.67	55.09	55.38	-2.36^**	-4.26	55.67	-2.65^**	-4.76
	C5	54.00	55.67	54.84	-2.39^**	-4.36	55.67	-3.22^**	-5.78
	C6	54.00	56.35	55.18	-1.93^**	-3.50	56.35	-3.10^**	-5.50
	C7	56.41	54.00	55.21	-1.75^**	-3.17	56.41	-2.95^**	-5.23
	C8	48.22	55.09	51.66	-0.60	-1.16	55.09	-4.03^**	-7.32
	C9	48.22	54.00	51.11	1.44^**	2.82	54.00	-1.45^**	-2.69
比叶重 SLW(g·cm^-2)	C1	0.008 4	0.008 0	0.008 2	0.0015^**	18.29	0.008 4	0.001 3^**	15.48
	C2	0.008 4	0.007 8	0.008 1	0.0019^**	23.46	0.008 4	0.001 6^**	19.05
	C3	0.008 8	0.008 4	0.008 6	-0.0011^**	-12.79	0.008 8	-0.001 3^**	-14.77
	C4	0.008 8	0.007 8	0.008 3	0.0001	1.20	0.008 8	-0.000 4^**	-4.55
	C5	0.008 4	0.008 8	0.008 6	0.0014^**	16.28	0.008 8	0.001 2^**	13.64
	C6	0.008 4	0.007 0	0.007 7	0.0023^**	29.87	0.008 4	0.001 6^**	19.05
	C7	0.008 0	0.008 4	0.008 2	0.0017^**	20.73	0.008 4	0.001 5^**	17.86
	C8	0.010 5	0.007 8	0.009 1	0.0011^**	12.09	0.010 5	-0.000 3	-2.86
	C9	0.010 5	0.008 4	0.009 4	0.0004	4.26	0.010 5	-0.000 7^**	-6.67
叶绿素a Chla(mg·g^-1)	C1	0.47	0.45	0.46	-0.02	-4.35	0.47	-0.03	-6.38
	C2	0.47	0.31	0.39	0.04^**	10.26	0.47	-0.04^*	-8.51
	C3	0.38	0.36	0.37	0.04^**	10.81	0.38	0.03	7.89
	C4	0.38	0.31	0.35	0.04^**	11.43	0.38	0.01	2.63
	C5	0.36	0.38	0.37	0.06^**	16.22	0.38	0.05^**	13.16
	C6	0.36	0.36	0.36	0.00	0.00	0.36	0.00	0.00
	C7	0.45	0.36	0.41	0.00	0.00	0.45	-0.04^*	-8.89
	C8	0.31	0.31	0.31	0.14^**	45.16	0.31	0.14^**	45.16
	C9	0.31	0.36	0.34	0.11^**	32.35	0.36	0.09^**	25.00
叶绿素b Chlb(mg·g^-1)	C1	0.25	0.24	0.25	-0.02^**	-8.00	0.25	-0.02^**	-8.00
	C2	0.25	0.18	0.22	0.01	4.55	0.25	-0.02^**	-8.00
	C3	0.22	0.21	0.22	0.00	0.00	0.22	0.00	0.00
	C4	0.22	0.18	0.20	0.01^**	5.00	0.22	-0.01	-4.55
	C5	0.21	0.22	0.22	0.01	4.55	0.22	0.01	4.55
	C6	0.21	0.20	0.21	0.00	0.00	0.21	0.00	0.00
	C7	0.24	0.21	0.23	-0.01	-4.35	0.24	-0.02^**	-8.33
	C8	0.20	0.18	0.19	0.05^**	26.32	0.20	0.04^**	20.00
	C9	0.20	0.21	0.21	0.04^**	19.05	0.21	0.04^**	19.05
叶绿素总量 Chla+b(mg·g^-1)	C1	0.72	0.68	0.70	-0.03	-4.29	0.72	-0.05^*	-6.94
	C2	0.72	0.49	0.61	0.06^**	9.84	0.72	-0.05^*	-6.94
	C3	0.60	0.57	0.59	0.04	6.78	0.60	0.03	5.00
	C4	0.60	0.49	0.55	0.05^**	9.09	0.60	0.00	0.00
	C5	0.57	0.60	0.59	0.07^**	11.86	0.60	0.06^**	10.00
	C6	0.57	0.56	0.57	0.00	0.00	0.57	0.00	0.00
	C7	0.68	0.57	0.63	0.00	0.00	0.68	-0.05^*	-7.35
	C8	0.50	0.49	0.50	0.20^**	40.00	0.50	0.20^**	40.00
	C9	0.50	0.57	0.54	0.16^**	29.63	0.57	0.13^**	22.81
类胡萝卜素 Cx.c(mg·g^-1)	C1	0.54	0.43	0.49	0.03	6.12	0.54	-0.02	-3.70
	C2	0.54	0.27	0.41	0.10^**	24.39	0.54	-0.03	-5.56
	C3	0.39	0.34	0.37	0.08^**	21.62	0.39	0.06^*	15.38
	C4	0.39	0.27	0.33	0.11^**	33.33	0.39	0.05^*	12.82
	C5	0.34	0.39	0.37	0.10^**	27.03	0.39	0.08^**	20.51
	C6	0.34	0.37	0.36	0.01	2.78	0.37	0.00	0.00
	C7	0.43	0.34	0.39	0.07^*	17.95	0.43	0.03	6.98
	C8	0.29	0.27	0.28	0.24^**	85.71	0.29	0.23^**	79.31
	C9	0.29	0.34	0.32	0.17^**	53.13	0.34	0.15^**	44.12

Table 4

Table 5

Table 6

Table 7

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性状 Trait	方差分量 Variance components			方差分量百分比/ % Percentage of variance portion			表型分化系数 V_ST(%)
性状 Trait	组合间 Among combinations	组合内 Within combinations	随机误差 Random errors	组合间 Among combinations	组合内 Within combinations	随机误差 Random errors	表型分化系数 V_ST(%)
叶长LL（cm）	0.784 0	1.742 0	0.604 0	25.058	55.646	19.296	31.05
叶宽LW（cm）	0.227 0	0.324 0	0.055 0	37.430	53.455	9.115	41.18
叶厚LT（mm）	0.003 3	0.001 4	0.000 1	69.051	28.432	2.517	70.83
叶形指数LSI	0.015 0	0.060 0	0.026 0	14.781	59.182	26.037	19.98
叶面积LA（cm²）	99.897 0	125.941 0	25.980 0	39.670	50.013	10.317	44.23
叶柄长PL（mm）	0.004 0	0.047 0	0.048 0	4.045	47.224	48.731	7.89
叶鲜重LFW（g）	0.053 0	0.082 0	0.021 0	34.172	52.269	13.559	39.53
叶干重LDW（g）	0.016 0	0.017 0	0.005 0	42.274	44.357	13.369	48.80
含水率LMC（%）	0.000 2	0.000 4	0.000 2	31.428	45.206	23.366	41.01
比叶重SLW（g·cm^-2）	0.000 000 8	0.000 000 7	0.000 000 5	38.829	33.722	27.449	53.52
叶绿素a Chla（mg·g^-1）	0.000 7	0.004 8	0.001 1	10.365	75.546	14.089	12.06
叶绿素b Chlb（mg·g^-1）	0.000 1	0.000 7	0.000 1	13.681	68.796	17.523	16.59
叶绿素总量Chla+b（mg·g^-1）	0.001 4	0.009 2	0.002 0	11.275	72.949	15.776	13.39
类胡萝卜素Cx.c（mg·g^-1）	0.002 0	0.013 0	0.004 0	9.811	68.844	21.345	12.47
平均Mean				27.2764	53.974 4	18.750	32.32

性状 Trait	叶长 LL	叶宽 LW	叶形指数 LSI	叶面积 LA	叶柄长 PL	叶鲜重 LFW	叶干重 LDW	含水率 LMC	比叶重 SLW	叶厚 LT	叶绿素a Chla	叶绿素b Chlb	叶绿素总量 Chla+b	类胡萝卜素 Cx.c
LL	1.000
LW	0.604^**	1.000
LSI	0.123	-0.698^**	1.000
LA	0.828^**	0.911^**	-0.408^**	1.000
PL	0.326^**	0.174^**	0.063	0.243^**	1.000
LFW	0.757^**	0.748^**	-0.267^**	0.884^**	0.266^**	1.000
LDW	0.736^**	0.724^**	-0.249^**	0.861^**	0.270^**	0.978^**	1.000
LMC	-0.067	0.042	-0.131^*	-0.20	-0.075	-0.087	-0.277^**	1.000
SLW	0.184^**	0.002	0.172^**	0.154^*	0.185^**	0.545^**	0.619^**	-0.526^**	1.000
LT	0.006	-0.137^*	0.197^**	-0.145^*	-0.098	-0.246^**	-0.276^**	0.153^*	-0.318^**	1.000
Chla	0.154^*	0.149^*	-0.050	0.185^**	0.014	0.163^*	0.169^**	-0.109	0.060	-0.094	1.000
Chlb	0.189^**	0.184^**	-0.060	0.211^**	0.052	0.206^**	0.212^**	-0.114	0.089	-0.105	0.955^**	1.000
Chla+b	0.166^**	0.160^*	-0.052	0.198^**	0.025	0.178^**	0.184^**	-0.112	0.070	-0.100	0.996^**	0.975^**	1.000
Cx.c	0.126^*	0.095	-0.006	0.135^*	-0.009	0.116	0.136^*	-0.157^*	0.071	-0.039	0.938^**	0.859^**	0.926^**	1.000

参数Paraterment		主成分Principal component
参数Paraterment		Y₁	Y₂	Y₃	Y₄
特征值Eigenvalue		5.19	3.38	1.86	1.31
累计贡献率 Comulate contribution rate(%)		37.06	61.20	74.51	83.88
特征向量 Eigenvector	叶长LL	0.319	-0.186	-0.014	0.445
	叶宽LW	0.351	-0.191	-0.318	-0.093
	叶厚LT	-0.125	0.039	-0.208	0.557
	叶形指数LSI	-0.145	0.070	0.411	0.574
	叶面积LA	0.377	-0.216	-0.195	0.079
	叶柄长PL	0.139	-0.107	0.184	0.299
	叶鲜重LFW	0.379	-0.236	0.042	0.015
	叶干重LDW	0.381	-0.231	0.135	-0.031
	含水率LMC	-0.078	-0.016	-0.513	0.170
	比叶重SLW	0.167	-0.113	0.575	-0.160
	叶绿素a Chl·a	0.254	0.440	-0.015	0.013
	叶绿素b Chl·b	0.264	0.413	-0.005	0.019
	叶绿素总量Chla+b	0.260	0.436	-0.011	0.014
	类胡萝卜素Cx.c	0.228	0.434	0.011	0.030

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Leaf Phenotype Variation and Heterosis in F₁ Progeny of Cross Between Castanea mollissima and C.henryi

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编号 No.	杂交组合 Hybrid combination	株数(株) Number of plants
C1	YLZ 26号×YLZ 14号	14
C2	YLZ 26号×YLZ 15号	30
C3	YLZ 24号×‘YLZ 1号	30
C4	YLZ 24号×YLZ 15号	30
C5	YLZ 1号×YLZ 24号	30
C6	YLZ 1号×YLZ 2号	17
C7	YLZ 14号×YLZ 1号	24
C8	魁栗×YLZ 15号	30
C9	魁栗×YLZ 1号	30
总计Total		235

编号 No.	主成分值 Principal component values				综合评价F值 Comprehensive evaluation value	排名 Ranking
编号 No.	F1	F2	F3	F4	综合评价F值 Comprehensive evaluation value	排名 Ranking
C2-53	2.09	5.51	3.77	-0.86	3.01	1
C9-102	5.66	0.78	0.60	0.86	2.92	2
C9-19	4.62	3.10	-1.54	0.47	2.74	3
C9-28	5.28	0.89	1.04	-0.58	2.69	4
C6-29	4.55	3.52	-2.06	-0.51	2.64	5
C8-20	1.98	4.15	2.92	0.15	2.55	6
C2-52	1.88	5.01	0.20	1.07	2.42	7
C8-35	3.91	1.92	0.06	0.87	2.39	8
C8-56	5.97	-1.00	0.49	-0.47	2.38	9
C9-111	2.15	4.37	1.09	-1.57	2.21	10
C2-56	2.66	3.63	0.09	-0.57	2.17	11
C8-54	5.37	-0.91	0.40	-0.58	2.11	12
C8-31	4.96	-0.87	0.75	0.31	2.10	13
C9-72	4.10	0.91	-0.78	0.66	2.03	14
C8-81	4.09	1.33	-0.48	-1.12	1.99	15
C5-66	0.95	5.06	-0.23	0.22	1.87	16
C8-40	2.06	2.24	0.71	1.25	1.81	17
C2-58	3.10	0.04	1.33	1.64	1.78	18
C8-59	4.82	-0.2	-1.79	-0.55	1.73	19
C5-23	2.68	2.03	0.43	-1.11	1.71	20
C9-22	4.48	-0.54	-1.27	0.48	1.68	21
C9-11	4.80	-1.25	-0.18	-0.99	1.62	22
C9-74	2.29	1.85	0.08	0.15	1.57	23
C9-41	3.55	-0.62	-0.26	0.34	1.39	24
C7-2	3.65	-1.25	0.46	0.38	1.37	25
C2-66	2.56	1.36	-0.57	-0.68	1.35	26
C9-116	2.85	0.05	1.00	-1.17	1.30	27
C5-75	0.92	1.53	2.05	0.99	1.28	28
C1-14	1.22	2.28	1.00	-0.68	1.28	29
C9-97	1.95	1.55	-0.06	-0.35	1.26	30
C5-31	2.06	0.24	0.24	1.60	1.20	31
C8-65	3.66	-0.93	-1.11	-0.23	1.15	32
C6-20	1.63	-0.17	2.28	0.97	1.14	33
C8-82	3.22	-2.08	1.23	0.65	1.09	34
C8-24	3.19	-1.30	0.36	-0.09	1.08	35
C1-61	1.26	1.84	-0.51	0.62	1.08	36
C8-15	2.41	0.34	0.10	-0.98	1.07	37
C8-63	2.81	0.38	-1.23	-0.74	1.07	38
C4-115	1.31	0.45	-0.36	3.60	1.05	39
C1-6	2.29	1.96	-2.06	-1.99	1.03	40