Functional Analysis of a Zinc-finger-like Gene from Nicotiana tabacum

doi:10.7525/j.issn.1673-5102.2012.04.010

Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (4): 430-436.doi: 10.7525/j.issn.1673-5102.2012.04.010

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Functional Analysis of a Zinc-finger-like Gene from Nicotiana tabacum

QU Guan-Zheng;ZHENG Tang-Chun;MA Lin;SHAO Long-Ting;CAO Feng-Juan;LI Kai-Long*

1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin　150040；2.Linjiang Forestry Bureau of Jilin Province,Linjiang　134600

Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
Contact: LI Kai-Long
Supported by:

Abstract

Abstract: In the present study, a Zinc-finger-like cDNA (NtZFL) was cloned from Nicotiana tabacum, the gene open reading frame (ORF) was 321 bp in length and encoded a predicated polypeptide of 106 amino acids. Expression of NtZFL in tobacco plant in response to biotic and abiotic stress was studied by QRT-PCR, the results showed that NtZFL was responsive to the treatments of MV, H₂O₂, ABA and cold. Northern blot analysis showed that NtZFL gene was expressed in different tissues of tobacco plant, achieving maximum in the young leaves and flowers. Subcellular localization analysis showed that the NtZFL protein was localized in the cell wall. GUS activity driven by NtZFL promoter was expressed in the whole of the young plant, but strongly detected in roots and leaf veins.

Key words: Nicotiana tabacum, NtZFL, stress, subcellular localization, promoter

CLC Number:

Q786

QU Guan-Zheng;ZHENG Tang-Chun;MA Lin;SHAO Long-Ting;CAO Feng-Juan;LI Kai-Long*. Functional Analysis of a Zinc-finger-like Gene from Nicotiana tabacum[J]. Bulletin of Botanical Research, 2012, 32(4): 430-436.

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Functional Analysis of a Zinc-finger-like Gene from Nicotiana tabacum

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