Abstract: To search new anti-infective from Scrophularia ningpoensis. Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data(UV,IR,MS,NMR) and identified by comparing with the authentic substance eight comounds were isolated from ethyl alcohol extract, They are identified as: 14-deoxy-12(R)-sulfo andrographolide(Ⅷ),cinnamic acid(Ⅰ),p-hydroxycinnamic acid(Ⅱ),succinic acid(Ⅲ),β-sitosterol(Ⅳ),daucosteol(Ⅴ),gluose(Ⅵ),ursolic acid(Ⅶ).Ⅷ was found first time in Scrophularia ningpoensis.

Key words: Scrophularia ningpoensis Hemsl, diterpenoid, 14-deoxy-12(R)-sulfo andrographolide