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Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (4): 460-464.doi: 10.7525/j.issn.1673-5102.2007.04.017

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Cloning and Characterization of Beclin1-like Gene in Eupatorium adenophorum Buds

WANG Min;LIU Ying;LI Si-Bin;JIANG Yang;QI Chang-Qing;WANG Li;ZU Yuan-Gang*   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20
  • Contact: ZU Yuan-Gang
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Abstract: It was found in the present study that programmed cell death (PCD) took place during the tapetum degenerating and pollen developing by microscopical observation and DNA ladder detection in Eupatorium adenophorum buds. Simultaneously, Beclin1-like gene was cloned from E. adenophorum buds at four different growth stages by RT-PCR method. The partial Beclin1-like gene (679 bp) was 98% similar to that of tobacco leaves (AY701316). Northern blotting showed that the expression of Beclin1-like gene in the stage Ⅱ was the strongest. The Beclin1-like gene was cloned in E. adenophorum buds for the first time and some relationships between the Beclin1-like Gene and PCD during the procreation of E. adenophorum were demonstrated. In addition, the results provided a new idea to further explore the invading mechanism of E. adenophorum.

Key words: Eupatorium adenophorum, buds, programmed cell death(PCD), Beclin1, Northern blotting analysisaf

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