Abstract: For establishing an efficient method in vitro rooting protocol for regenerated shoos of Begonnia×Rieger and decreasing the cost of rooting, the effects of basal medium strength, white sugar concentration and agar concentration were investigated in this study.The results showed that the strength of basal medium and white sugar concentration produced more effects on rooting. Considering rooting frequency and rooting quality, 1/4MS basal medium and 15 g·L^-1 white sugar were the best choice for rooting of Begonnia×Rieger. In all treatment of different agar concentration, there were no obvious difference in rooting frequency and rooting quality, for decreasing the cost, we selected 3 g·L^-1 agar as the best concentration for rooting.

Key words: Begonnia×Rieger, regenerated shoots, in vitro rooting