Abstract: For improving the rooting percentage and providing an optimal technology for seedling production, we studied the rooting technology of in vitro and ex vitro of Acacia mangium×A.auriculiformis hybrid by using the proliferation seedlings as materials (in vitro rooting with the proliferation cultured for 30 d, 2-3 cm seedling; and ex vitro rooting with the proliferation cultured for 45 d, 3-5 cm seedlings), and compared the economic benefits of two techniques for seedling production. Inoculating the aseptic plants which had been multiplication cultured for 30 d to 1/2MS with combination of 1.0 mg·L^-1 IBA, 0.5 mg·L^-1 NAA, 3% sucrose could get the highest rooting rate of 99.43%, and the survival rate was 98.0% after transplanted to the greenhouse 15 d. However, when immersing the aseptic seedlings which had been multiplication cultured for 45 d with 100 mg·L^-1 IBA for 1 hour, the rooting rate was 94.67%. For producing 10 000 seedlings, the economic benefit of ex vitro rooting was more than RMB 1 288.26 Yuan than that of in vitro rooting when the benefit was considerable. The ex vitro rooting technique was more available for the seedling production of Acacia mangium×A.auriculiformis.

Key words: Acacia mangium×A.auriculiformis, in vitro rooting, ex vitro rooting, economic benefit