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Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (3): 343-346.

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Athyriaceae plant DNA isolation and the effects on RAPD analysis

ZHANG Xiu-Ying, FAN Ya-Wen   

  1. Department of Biological, Harbin Normal University, Harbin 150080
  • Received:2003-11-28 Online:2004-09-15 Published:2016-06-14

Abstract: 4 species of Athyriaceae from Mudanjiang,Heilongjiang Province have been used for the isolation of the DNA and the analysis of RAPD.In the process of testing the content of DNA templates,DNA polymerase,primers,Mg2+ and dNTP,a suitable way has been found that very useful for isolating the DNA of Pteridophyta and its RAPD analyzing.The result suggest that the new way can be a good start for RAPD analysis in the Athyriaceae genetic diversity research.

Key words: Athyriaceae, RAPD, random primer