Cytological investigation on microsporogenesis of cytoplasmic male sterility in radish(Raphanus sativus L.)

Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (3): 305-308.

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Cytological investigation on microsporogenesis of cytoplasmic male sterility in radish(Raphanus sativus L.)

HUANG Hao^1,2, LIU Li-Wang², CHEN Chong-Shun¹, GONG Yi-Qin², SONG Xian-Yong², WEI Kai-Yu²

1. College of Life science, Nanjing Normal University, Nanjing 210097;
2. National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing 210095

Received:2004-01-07 Online:2004-09-15 Published:2016-06-14

Abstract

Abstract: The cytology of the microsporogenesis and development of anther wall of cytoplasmic male sterile (CMS) lines A₂,A₄ and its maintainer lines B₂,B₄ was studied.The results revealed that the tapetal cells of the male sterile line A₂ developed abnormally at tetrad stage,and they became vacuolated and numerous small vacuoles are present;furthermore,the small vacuoles fused into large ones.Exine of microspore was light stained,and cell wall was destructed.Finally with degeneration of the tapetal cells,the pollen aborted.The tapetal cells of CMS line A₄ enlarged abnormally at meiotic phase,pressing microsporocyte seriously.Lately,light-staining exine of microspore could not be observed,and tapetal cells fused to form big lump invading the loculi,and finally leading to pollen grain abortion.

Key words: Raphanus sativus, cytoplasmic male sterility, microsporogenesis, cytomorphology

HUANG Hao, LIU Li-Wang, CHEN Chong-Shun, GONG Yi-Qin, SONG Xian-Yong, WEI Kai-Yu. Cytological investigation on microsporogenesis of cytoplasmic male sterility in radish(Raphanus sativus L.)[J]. Bulletin of Botanical Research, 2004, 24(3): 305-308.

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Cytological investigation on microsporogenesis of cytoplasmic male sterility in radish(Raphanus sativus L.)

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