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Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (1): 107-110.

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Study on DNA extraction of Zelkova

JIN Xiao-Ling1,2, WU YUN Ta-Na2, ZHANG Zhi-Jun2, HE Ping2,3   

  1. 1. Zhejiang Normal University, Jinhua 321004;
    2. The Key Laboratory of Non-timber Breeding and Cultivation of National Forestry Ministry, Center-South Forestry University, Zhuzhou 412006;
    3. International Engineering Consulting Corporation, Dept.Agriculture, Forestry and Water, Beijing 100044
  • Received:2003-06-17 Online:2004-03-15 Published:2016-06-14

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Abstract: The content of phenolic compound, polysaccharides and other secondary metabolites is high in ligneous plant, which influences frequently the yield and quality of genome DNA when being extracted.In this study, a new method was introduced which do best for characterization the purity and concentration of genome DNA.The result showed that it could effectively eliminate the affections of the secondary materials to extracted genome DNA from Zelkova, and which be suitable for RAPD analysis and other geneticanalysis.

Key words: Zelkova, extraction of genome DNA, RAPD