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Bulletin of Botanical Research ›› 2001, Vol. 21 ›› Issue (2): 272-277.

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GENETIC DIFFERENTIATION IN LEYMUS CHINENSIS POPULATIONS REVEALED BY RAPD MARKERS I:VARIATION ON THE FREQUENCIES OF AMPLIFIED FRAGMENTS

CUI Ji zhe1,2, ZU yuan gang1, GUAN Xiao duo3   

  1. 1. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China;
    2. Department of Biology, Harbin Normal University, Harbin 150080, China;
    3. The Research
  • Received:2000-12-10 Online:2001-06-15 Published:2016-06-14

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Abstract: Leymus chinensis is a rhizome grass species, which has 2 leaf-colored types, a grayish green and a yellowish green, in the Songnen Plain of China. To reveal the molecular bases of genetic differentiation between the 2 types, RAPD was conducted on 105 individuals from 9 populations by using 15 primers. RAPD produced 155 amplified fragments, and total percentage of polymorphic loci is 96.1. 10.1% polymorphic loci were different between the 2 types. There were similarities of certain polymorphic loci among the populations sampled in the similar habitat. There were characterized polymorphic loci for 9 populations respectively.

Key words: Leymus chinensis, genetic differentiation, RAPD