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Bulletin of Botanical Research ›› 1999, Vol. 19 ›› Issue (2): 63-68.

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EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS

Zhang Heng-qing1, An Li-jia2, Zu Yuan-gang1   

  1. 1. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry Univ., Harbin 150040;
    2. Chemical Institute, Dalian Science and Technology Univ., Dalian 116001
  • Received:1997-03-10 Online:1999-06-15 Published:2016-06-14

Abstract: The PCR reaction system by Promega was employed. The DNA templates of Pinus koraiensis extracted from young needles were amplified in RAPD. The effect of content of Mg2+, dNTP, DNA templates, primers and DNA polymorase on experimental results were tested and the optimal reaction system of RAPD for korean pine was determined.

Key words: Pinus koraiensis, RAPD, Composition