Abstract: Cytochemical localization of glycoproteins at the cell surface in the leaves of wheat was observed by electron microscopy using ruthenium red changes in the cell surface, glycoproteins in cold hardened.the thickness of glycoproteins layer seemed to have increased during the seedlings hardened at 2-3℃.prolong the time of cold hardened the glycoproteins layer appeared to have moved into the intercellular spaces and thinner.the glycoproteins at the cell surface in hardy winter wheat seedlings appeared richer than those of non-hardy spring wheat seedling.the results mentioned above suggested that plant cell surface may be closely associated with the plant's ability to resist cold hardiness.

Key words: Triticum aestivum L., Glycoprotein, Cytochemistry, Resist cold hardiness