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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (3): 339-345.doi: 10.7525/j.issn.1673-5102.2013.03.014

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Nano-paclitaxel Transport by Human Intestinal Epithelial Caco-2 Cells

XU Chao;;JIANG Shou-Gang;;GONG Xian-Feng;ZU Yuan-Gang;*   

  1. 1.State Engineering Laboratory of BioResources EcoUtilization,Northeast Forestry University,Harbin 150040;2.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040;3.Engineering Research Center of Forest Biopreparation,Ministry of Education,Northeast Forestry University,Harbin 150040;4.Institute of Chemistry and Materials Science,Heilongjiang University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: ZU Yuan-Gang
  • Supported by:

Abstract: Many studies had proved that oral administration of paclitaxel was poorly absorbed and easy to cause hypersensitivity reactions. For this reason, we produced a new nano-paclitaxel by paclitaxel granule wrapped with mannitol and Human Serum Albumin (HSA), which had better water solubility and lower systemic toxicity. In order to research the absorption of nano-paclitaxel after oral administration, the transepithelial flux of nano-paclitaxel was studied using the human colonic cell line Caco-2 cells. The flux of nano-paclitaxel (at a concentration range of 0.5-20 μmol·L-1) across the Caco-2 cell layer was linear with time for up to 3 h. The apparent permeability coefficient (Papp) for the apical to basolateral transport of nano-paclitaxel (20.9±2.1×10-6 cm·s-1; N=3) and the apparent maximal concentration (KM) of the active efflux component (14.00 μmol·L-1) were relatively high, suggesting that nano-paclitaxel may have a better absorption rate in the intestinal tract.

Key words: nano-paclitaxel, Caco-2 cell, transport absorption, P-glycoprotein

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