The Responses of Rhizosphere Soil of Caragana korshinskii under Soil Sterilization, AM Fungi Inoculation and N Addition

doi:10.7525/j.issn.1673-5102.2024.04.011

Abstract

Abstract:

Based on the fact that soil microorganisms can enhance the adaptability of plants to global change， the effects of soil microorganisms on the ecological stoichiometric characteristics of rhizosphere soil microorganisms were studied under the background of nitrogen deposition， and then the regulation of changes in ecological stoichiometric characteristics on the soil microbial nutrient limitation was explored. Under the premise of soil sterilization treatment（sterilized soil（+S） and non-sterilized soil（-S））， one-year-old potted seedlings of Caragana korshinskii were inoculated in two ways（inoculation（+M） and without inoculation（-M））， and nitrogen addition treatments（no nitrogen application（CK）， low nitrogen（LN， 3 g·m^-2·a^-1）， high nitrogen（HN， 6 g·m^-2·a^-1）） were set up. Compared with the original microbial community composition in non-sterilized soil without fungus inoculation， the correlation between ecological stoichiometric characteristics of microhabitat and microbial nutrient limitation under different treatments was explored.Results were as follows：（1）In sterilized soil treatment， the relative abundance of fungi in the rhizosphere soil of C. korshinskii mycorrhizal seedlings was significantly increased by 82.20%， 25.00% and 59.84% under CK， LN and HN treatments than that of non-mycorrhizal seedlings， respectively. （2）In the treatment of non-sterilized soil， the microbial biomass carbon-to-phosphorus ratio in the rhizosphere soil of non-mycorrhizal C. korshinskii seedlings under LN treatment was lower by 46.28% compared to CK treatment（P<0.05）， while that of mycorrhizal seedlings was 56.76% higher compared to CK treatment（P<0.05）. （3）Under the nitrogen addition treatments， stoichiometry of carbon and nitrogen-related soil enzyme activity， stoichiometry of carbon and phosphorus-related soil enzyme activity， and vector length in the rhizosphere soil of C. korshinskii non-mycorrhizal seedlings under sterilized soil treatment were significantly lower than those of mycorrhizal seedlings（P<0.05）. （4）PLS-PM path analysis showed that the total effect coefficient of microbial regulation on microbial nutrient limitation was greater than that of nitrogen addition treatment. In summary， soil indigenous microorganisms and mycorrhizal fungi synergistically regulated the enzymatic stoichiometric ratio of rhizosphere soil， and enhanced the adaptation of soil microbial nutrient limitation to nitrogen addition.

Key words: Caragana korshinskii, microbial regulation, nitrogen addition, ecological stoichiometry, microbial nutrient limitation

CLC Number:

Q938.13

Yixue LI, Dongmei YE, Longfei HAO, Tingyan LIU, Jiajing DUAN, Zhengying NIE. The Responses of Rhizosphere Soil of Caragana korshinskii under Soil Sterilization, AM Fungi Inoculation and N Addition[J]. Bulletin of Botanical Research, 2024, 44(4): 590-601.

Figures/Tables 7

Table 1

Fig.1

Table 2

Table 3

Enzymatic activities and its stoichiometric characteristics in rhizosphere soil of C. korshinskii seedings under soil sterilization， seedling inoculation and nitrogen addition treatments

处理Treatments			E_C/（nmol·g^-1·h^-1）	E_N/（nmol·g^-1·h^-1）	E_P/（nmol·g^-1·h^-1）	E_C∶E_N	E_C∶E_P	E_N∶E_P	V_L	V_A/（°）	E_C∶E_N∶E_P
+S	-M	CK	2.41±0.29f	5.90±0.59g	7.49±0.14e	0.48±0.04d	0.43±0.06d	0.88±0.04f	0.65±0.07d	48.84±1.48a	1∶2∶2
		LN	2.58±0.23f	19.16±2.64f	8.36±0.48e	0.32±0.02e	0.44±0.04d	1.38±0.03abc	0.55±0.04d	35.90±0.51def	1∶3∶2
		HN	2.53±0.09f	15.51±0.80f	8.23±0.99e	0.34±0.01e	0.44±0.01d	1.31±0.06cd	0.55±0.01d	37.33±0.22cd	1∶2∶2
	+M	CK	6.75±0.87e	15.28±1.80f	8.25±0.39e	0.70±0.02b	0.89±0.04bc	1.29±0.03cd	1.13±0.04bc	37.90±0.41cd	1∶1∶1
		LN	6.66±0.20e	12.95±0.70fg	9.64±0.59e	0.74±0.01b	0.84±0.01c	1.13±0.01e	1.12±0.02bc	41.48±0.18b	1∶1∶1
		HN	7.31±0.82e	14.43±2.18f	8.80±1.40e	0.75±0.01b	0.92±0.06bc	1.23±0.06d	1.19±0.05bc	39.12±1.42c	1∶1∶1
-S	-M	CK	19.79±0.14a	40.63±2.13e	17.96±0.37d	0.81±0.01a	1.03±0.01a	1.28±0.01cd	1.31±0.01a	37.96±0.22cd	1∶1∶1
		LN	19.22±1.60ab	54.92±0.99d	23.15±2.24b	0.74±0.02b	0.94±0.01ab	1.28±0.03cd	1.19±0.02b	38.00±0.53cd	1∶1∶1
		HN	14.57±0.36d	66.62±6.35c	19.04±0.99cd	0.64±0.01c	0.91±0.01bc	1.42±0.02ab	1.11±0.01bc	35.09±0.12ef	1∶1∶1
	+M	CK	16.48±0.69cd	77.26±3.91b	19.40±1.46cd	0.64±0.00c	0.95±0.01ab	1.47±0.02a	1.15±0.01bc	34.25±0.22f	1∶1∶1
		LN	16.38±0.19cd	81.62±2.30ab	22.03±0.15bc	0.63±0.00c	0.90±0.00bc	1.42±0.01ab	1.10±0.00bc	35.09±0.01ef	1∶1∶1
		HN	17.50±0.77bc	88.12±2.64a	27.50±1.67a	0.64±0.01c	0.86±0.00bc	1.35±0.01bc	1.08±0.01c	36.47±0.15de	1∶1∶1
灭菌处理Sterilization treatment（S）			***	***	***	***	***	***	***	***
接菌处理Inoculation treatment（M）			***	***	**	***	***	**	***	***
氮添加处理Nitrogen addition（N）			NS	***	**	***	NS	***	**	***
灭菌处理×接菌处理（S×M）			***	***	NS	***	***	NS	***	NS
灭菌处理×氮添加处理（S×N）			*	**	NS	NS	*	***	NS	***
接菌处理×氮添加处理（M×N）			**	**	*	***	NS	***	NS	***
灭菌处理×接菌处理×氮添加处理（S×M×N）			*	NS	**	*	NS	***	NS	***

Table 3

Table 4

Fig.2

Fig.3

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施入时间 Application time	累计施入次数 Cumulative application times	低氮处理 Low nitrogen treatment （LN）	高氮处理 High nitrogen treatment （HN）
6月 June	1	0.16	0.31
	2	0.16	0.31
	3	0.16	0.31
7月 July	4	0.33	0.66
	5	0.33	0.66
	6	0.33	0.66
8月 August	7	0.37	0.73
	8	0.37	0.73
	9	0.37	0.73
9月 September	10	0.22	0.45
9月 September	11	0.22	0.45

处理 Treatments			M_BC/（mg·kg^-1）	M_BN/（mg·kg^-1）	M_BP/（mg·kg^-1）	M_BC∶M_BN	M_BC∶M_BP	M_BN∶M_BP
+S	-M	CK	95.12±12.78cd	1.81±0.02f	2.28±0.41bcd	52.50±7.26a	42.69±2.84bc	0.86±0.18ef
		LN	73.29±8.28d	3.87±0.84def	1.89±0.49cd	19.81±2.16cde	41.79±5.81bc	2.09±0.09de
		HN	70.85±14.74d	2.63±0.53def	2.95±0.40bcd	26.91±0.51c	23.66±1.99d	0.88±0.07ef
	+M	CK	85.03±14.15d	2.19±0.25ef	3.34±0.32b	38.72±3.19b	25.24±2.10d	0.65±0.02f
		LN	90.60±8.69cd	5.27±0.80cdef	3.13±0.46bcd	17.55±1.54cdef	29.59±3.23cd	1.68±0.05def
		HN	166.17±3.16a	7.24±1.29cd	3.46±0.24b	24.48±4.36cd	48.33±2.35b	2.07±0.28de
-S	-M	CK	142.28±24.25a	14.17±3.66b	1.75±0.25d	10.85±1.94efg	81.77±7.45a	7.83±1.02a
		LN	136.70±6.34ab	19.25±0.69a	3.15±0.28bc	7.10±0.16g	43.92±3.33b	6.18±0.39b
		HN	157.94±10.83a	17.28±1.23ab	3.04±0.61bcd	9.16±0.35fg	55.13±8.12b	6.01±0.78b
	+M	CK	100.79±3.79bcd	16.73±1.09ab	3.49±0.26b	6.05±0.23g	29.05±1.29cd	4.80±0.04c
		LN	127.76±9.01abc	6.77±1.47cde	2.93±0.57bcd	20.31±3.54cde	45.54±4.85b	2.31±0.24d
		HN	129.19±14.33abc	8.89±1.62c	5.03±0.50a	14.90±1.21defg	25.62±0.40d	1.75±0.17def
灭菌处理Sterilization treatment（S）			***	***	NS	***	***	***
接菌处理Inoculation treatment（M）			NS	*	***	NS	***	***
氮添加处理Nitrogen addition（N）			*	NS	**	***	NS	*
灭菌处理×接菌处理（S×M）			***	***	NS	**	***	***
灭菌处理×氮添加处理（S×N）			NS	*	NS	***	*	***
接菌处理×氮添加处理（M×N）			**	**	NS	**	***	NS
灭菌处理×接菌处理×氮添加处理（S×M×N）			*	**	NS	NS	***	NS

处理Treatments			D_OC/（mg·kg^-1）	A_N/（mg·kg^-1）	A_P/（mg·kg^-1）	D_OC∶A_N	D_OC∶A_P	A_N∶A_P
+S	-M	CK	3.78±0.07bc	5.95±0.33de	2.89±0.11bcd	0.64±0.02ef	1.31±0.02cde	2.05±0.03bc
		LN	3.63±0.22cd	7.38±0.28ab	3.05±0.13bc	0.49±0.02f	1.19±0.04de	2.42±0.01b
		HN	3.64±0.11cd	8.04±1.01a	2.53±0.07def	0.46±0.04f	1.44±0.01abc	3.16±0.32a
	+M	CK	3.29±0.19cd	2.44±0.05e	2.09±0.06f	1.35±0.05bc	1.57±0.04ab	1.17±0.01ef
		LN	2.86±0.22d	3.75±0.54de	2.09±0.08f	0.78±0.09ef	1.37±0.06bcd	1.79±0.21cd
		HN	3.11±0.10cd	3.69±0.04de	2.23±0.17ef	0.84±0.02de	1.41±0.06bc	1.67±0.10cd
-S	-M	CK	3.95±0.60bc	3.74±0.59de	2.65±0.19cde	1.23±0.06c	1.47±0.12abc	1.22±0.16ef
		LN	3.23±0.07cd	3.65±0.87de	2.52±0.23def	1.13±0.01cd	1.30±0.08cde	1.15±0.06ef
		HN	3.53±0.11cd	4.55±0.93cd	3.07±0.13bc	0.64±0.00ef	1.15±0.03e	1.79±0.03cd
	+M	CK	5.05±0.11a	3.36±0.14de	3.23±0.14b	1.61±0.05b	1.57±0.03ab	0.98±0.03fg
		LN	5.08±0.52a	3.09±0.69de	3.67±0.18a	2.02±0.26a	1.37±0.07bcd	0.70±0.09g
		HN	4.57±0.05ab	4.48±0.66cd	2.83±0.21bcd	1.11±0.21cd	1.63±0.11a	1.54±0.16de
灭菌处理Sterilization treatment（S）			***	***	***	***	NS	***
接菌处理Inoculation treatment（M）			*	***	NS	***	***	***
氮添加处理Nitrogen addition（N）			NS	*	NS	***	**	***
灭菌处理×接菌处理（S×M）			***	***	***	NS	NS	***
灭菌处理×氮添加处理（S×N）			NS	NS	NS	***	NS	**
接菌处理×氮添加处理（M×N）			NS	NS	NS	NS	NS	NS
灭菌处理×接菌处理×氮添加处理（S×M×N）			NS	NS	***	*	**	*

[1]	Zhiqi CHEN, Haina ZHANG, Jiali LIU, Xianghui LU, Baocheng YANG. Effects of Nitrogen Addition on Root Growth, Biomass Allocation and Non-structural Carbohydrate Content of Cinnamomum bodinier Seedlings in Rare Earth Tailings [J]. Bulletin of Botanical Research, 2024, 44(1): 86-95.
[2]	Yuhang SU, Xiaoqian SONG, Jingwen ZHENG, Zhonghua ZHANG, Zhonghua TANG. Ecological Stoichiometric Characteristics of C, N and P and Their Relationship with Soil Factors from Different Organs of the Halophytic Chenopodiaceae Plants in Hulunbuir [J]. Bulletin of Botanical Research, 2022, 42(5): 910-920.
[3]	ZHANG Shu-Qi, XU Quan, YAO Hai-Rong, YANG Qiu, LIU Wen-Jie, WANG Meng. Carbon, Nitrogen and Phosphorus Contents and Their Ecological Stoichiometry Characteristics in Leaves of Casuarina equisetifolia and Ipomoea pes-caprae in the Coastal Zone of Hainan Island [J]. Bulletin of Botanical Research, 2020, 40(2): 224-232.
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[5]	GUO Hao, ZHUANG Wei-Wei, LI Jin. Characteristics of Biomass and Stoichiometry of Four Desert Herbaceous Plants In the Gurbantunggut Desert [J]. Bulletin of Botanical Research, 2019, 39(3): 421-430.
[6]	TAN Hai-Xia, JIN Zhao-Guang, SUN Fu-Qiang, PENG Hong-Li, YANG Zhuo, GENG Shi-Gang. Stoichiometry Characteristics of Typical Halophytes in Luanhe Estuary Wetland [J]. Bulletin of Botanical Research, 2018, 38(6): 956-960.
[7]	ZHOU Yang, ZHANG Dan-Ju, SONG Si-Meng, LI Xun, ZHANG Yan, ZHANG Jian. Forest Gaps Size on Pinus massoniana Plantation of Three Natural Regeneration Herb N and P Stoichiometry [J]. Bulletin of Botanical Research, 2017, 37(6): 915-925.
[8]	GUO Hai-Xia, XU Bo, SHI Fu-Sun, WU Yan. Effects of Shading and Nitrogen Addition on the Growth and C-N Balance of Fritillaria unibracteata [J]. Bulletin of Botanical Research, 2017, 37(5): 738-743.