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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 573-587.doi: 10.7525/j.issn.1673-5102.2021.04.013

• Research report • Previous Articles    

Transcriptome Analysis of Petals and to Explore Related Genes in Petal Development of Camellia nitidissima

Bo LI, He-Xia LIU, Qin LIU, Xing-Wen ZHOU()   

  1. Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Yulin Normal University,Yulin 537000
  • Received:2020-06-02 Online:2021-07-20 Published:2021-03-24
  • Contact: Xing-Wen ZHOU E-mail:xingwenzhou2003@163.com
  • About author:LI Bo(1981—),male,PhD,associate professor,mainly engaged in plant resources utilization research.
  • Supported by:
    National Nature Science Foundation(31860228);Key Program of Guangxi Nature Science Foundation(2018GXNSFDA281007);Project of high-level talents in Yulin normal university(G2018025)

Abstract:

Petal size was one of the main factors affecting the ornamental value of Camellia nitidissima, but its formation mechanism was unclear. In this study, the petal development process of C. nitidissima was divided into five developmental stages: young bud stage(S1), early bud stage(S2), turning yellow stage(S3), semi-blooming stage(S4) and full bloom stage(S5) respectively. The dynamic changes of transcriptome during flower development were analyzed by RNA-seq technique. Using enrichment analysis and trend analysis of differentially expressed genes, it was found that the number of differentially expressed genes in auxin transduction pathway was the largest, and some auxin response genes such as AUX1/LAX cotransporters, AUX/IAA genes and SAURs were significantly up-regulated during flowering respectively, indicated that auxin was an important regulator of petal growth. Transcription factor genes such as MYBbHLH and zinc finger protein genes were obviously up-regulated or down-regulated respectively, and some downstream functional genes such as xyloglucan endotransglucosylase/hydrolase(XTH), pectin esterase(PE) and pectin lyase(PL) were also showed significant changes respectively. The results also suggested that these genes may be candidate genes involved in petal development. In addition, the expressions of some genes related to flowering regulation, such as FTSOC1AP3PISEP3, etc., were analyzed during the petal development of C. nitidissima, but the results showed that their expressions were mainly medium and low expression. Besides, the results of KEGG enrichment analysis of highly expressed genes revealed that the synthesis of secondary metabolites was accompanied by the whole petal development of C. nitidissima. All these results would lay a theoretical foundation for further researches of the regulation mechanism of petal development of C. nitidissima.

Key words: Camellia nitidissima, petal development, differentially expressed gene, trend analysis

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