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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (3): 416-420.doi: 10.7525/j.issn.1673-5102.2016.03.015

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Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata

WANG Meng-Liang1, JIAO Jin1,2, XING Jie3, TIAN Jun-Sheng3, CUI Jin-Long1, WANG Jun-Hong1   

  1. 1. Insititute of Applied Chemistry, Shanxi University, Taiyuan 030006;
    2. Insititute of Biotechnology, Shanxi University, Taiyuan 030006;
    3. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006
  • Received:2015-09-29 Online:2016-05-15 Published:2016-05-20

Abstract: We studied the effects on the key signal molecules and the main secondary metabolites's content in tissue culture of Rhodiola crenulata inoculated with endophic fungi ZPRa-R-1. We selected Greiss method, RP-HPLC and Titanium sulfate method to detect the contents of NO, SA and H2O2, respectively, and detected the key enzymes, PAL and CA4H by spectrophotometry. p-Tyrosol and salidroside in Rhodiola seedling co-cultured with fungi were evaluated by RP-HPLC. The content of NO increased firstly, followed by SA and H2O2 after that Rhodiola seedling inoculated with endophic fungi. The maximum contents of NO, SA and H2O2 were detected at 8, 10 and 12 d, and they were 0.193 μmol·g-1, 0.062 μg·g-1 and 0.092 μmol·g-1, respectively. The maximum contents of PAL and CA4H were 166.400 U·g-1, 0.625 U·g-1·h-1, and were 8 and 1.5 times as much as that of control, repectively. The contents of p-tyrosol and salidroside were accumulated, and the time of each of them reaching the maximum content was on 8 and 10 d, and the contents were 8.656 and 0.498 mg·g-1, and were 7 and 2.5 times as much as that of control, respectively. Therefore, ZPRa-R-1 has the capacity of regulating signals and promoting the activities of PAL and CA4H, and finally promoted accumulation of p-tyrosol and salidroside in co-culturing seedling of fungi and Rhodiola.

Key words: Rhodiola crenulata, endophytic fungi, signal molecule, p-tyrosol, salidroside

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