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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (4): 515-520.doi: 10.7525/j.issn.1673-5102.2015.04.007

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Sequence Analysis of ITS Regions and ndhA Gene for Determining Phylogenetic Relationship of Diospyros kaki(persimmon) With Other Related Wild Diospyros(Ebenaceae) Species

FU Jian-Min1,2;LIANG Jin-Jun1,2;WUYUN Tana1,2;LIANG Yu-Qin1,2;SUN Peng1,2;LI Fang-Dong1,2*   

  1. 1.China Paulownia Research Center,Zhengzhou 450003;
    2.Non-timber Forest Research and Development Center of CAF,Zhengzhou 450003
  • Online:2015-07-20 Published:2016-03-09
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Abstract: We used eight species(30 genotypes) of Diospyros spp. to study the interspecific relationship and intraspecific variation by ndhA and ITS sequences for calculating substitution model of two sequence and their combination, and then building three phylogenetic trees constructed by ML method based model parameters. The length range of ndhA sequence were 1492 to 1511, 14 informative sites, and the length range of ITS sequence were 660 to 761, 56 informative sites. The best fit models of ITS, ndhA and ndhA+ITS were(TrN+I+G), (F81+I) and (GTR+I+G), respectively. D.kaki was closely related to D.oleifera and D.artrotricha, but distant from D.virginiana and D.cathayensis. The length of D.kaki cultivar was 730, including four variation sites. We analyzed the genetic diversity of selected 21 D.kaki cultivars based on four variation sites. The results suggested that ndhA and ITS sequence variation analysis could explain the relationship between D.kaki and its related species, and the intraspecific variation could be analyzed according to four variation sites.

Key words: cpDNA, ndhA, ITS, Diospyros kaki, relationship

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