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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (6): 846-854.doi: 10.7525/j.issn.1673-5102.2020.06.007

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In vitro Germination,Callus Induction and Primary Haustorium Organogenesis in the Parasitic Plant Cynomorium songaricum

Xin YUE1,2, Gui-Lin CHEN1()   

  1. 1.School of Life Science,Inner Mongolia University,Hohhot 010021
    2.College of Pharmacy,Inner Mongolia Medical University,Hohhot 010021
  • Received:2019-12-20 Online:2020-11-20 Published:2020-11-04
  • Contact: Gui-Lin CHEN E-mail:guilinchen61@163.com
  • About author:YUE Xin(1983—),female,lecturer,Ph.D.,mainly engaged in biotechnology research on Traditional Chinese Medicine.
  • Supported by:
    Inner Mongolia Natural Science Foundation, PhD Foundation Project(2016BS0811);Inner Mongolia Science and Technology Innovation Guided Award Fund Project(2015cztxyd02);Specialized Research Fund for Doctoral Programs in Colleges and Universities(20091501110002);Inner Mongolia Science and Technology Achievements Transformation Project(CGZH2018127)


Cynomorium songaricum Rupr. is an obligate parasitic plant widely used as traditional Chinese medicine and Mongolian medicine. Here, we firstly describe protocols for in vitro germination, callus induction and haustorium organogenesis in C.songaricum Rupr. In this study, adequate concentrations of gibberellic acid(GA3),in combination with other plant growth regulators in the medium, promoted embryo development and germination of C.songaricum seeds. The highest callus induction rate from seeds(13.7%) after a 40 d incubation was obtained with B5 medium adding 2,4-dichlorophenoxyacetic acid(2,4-D; 1.0 mg·L-1), kinetin(KT;0.5 mg·L-1) and GA3(1.0 mg·L-1. This resulted in callusformation in 13.7%±3.1% of seeds. Addition of 2,4-D(0.5 mg·L-1) and KT(0.25 mg·L-1) yielded highest haustorium organogenesis from calluses. Some primary haustorium branched to form adventitious roots of 3-4 cm in length. Subsequently, the tip of each adventitious root formed a nascent primary haustorium, which was then branched out into adventitious roots. The role of auxins(2,4-D in this study) in the formation of primary haustorium and adventitious roots from seed callus in C.songaricum was also discussed.

Key words: medicinal plant, HIFs, histology, host-parasite relationship, cultivation

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