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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (3): 298-303.doi: 10.7525/j.issn.1673-5102.2005.03.013

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Protein changes induced by cadmium stress in three radish cultivar seedlings: a comparison using two-dimensional gel electrophoresis

FAN Bao-Li, WANG Zhen-Ying, PENG Yong-Kang   

  1. Department of Biology, Tianjin Normal University, Tianjin 300074
  • Received:2005-01-17 Online:2005-09-15 Published:2016-06-14
  • Supported by:
    Supported by foundation for university key science and technology by the ministry of education of China(02010)

Abstract: The germination rate was decreased to 92% when seeds were treated at 0.5 mmol·L-1 Cd2+ and was progressively decreased with higher Cd2+. 83% and 67% germination rates were observed respectively at 1 mmol·L-1 and 5 mmol·L-1 Cd2+ treatment in XWY, but the lower germination rate was observed with 5 mmol·L-1 Cd2+ treatment in XYB. The germination rate was decreased to 83% only at 0.05 mmol·L-1 Cd2+ and only 58% germination rate was observed when seeds were treated at 1 mmol·L-1 and 5 mmol·L-1 Cd2+ in WQ. The effects of Cd2+ on seedling growth were observed in three radish cultivars. Seedling growth damage was much more serious with higher Cd2+ concentration. WQ is the most sensitive to Cd2+ treatment according to the experiment results which were obtained by testing germination rate and seedling growth in three radish cultivars. 5 protein spots were disappeared and 15 new protein spots were induced in XWY seedling which were treated by 0.1 mmol·L-1 Cd2+, 2 protein spots were disappeared and 1 protein spot was decreased in content, 13 new protein spots were induced in XYB, 12 new protein spots were induced in WQ. A close positive relationship was observed between protein spots change and inhibited seedling growth in the three radish cultivars which were treated by 0.1 mmol·L-1Cd2+. It may be important for understanding biochemical mechanism of Cd2+ damage.

Key words: germination rate, two dimensional gel electrophoresis, protein spot, cadmium, Raphanus sativus L.