关键词: 光萼荷属, SSR, 正交实验设计, 反应体系优化, 指纹图谱

Abstract: The SSR reaction system was established by orthogonal design and the SSR fingerprints were constructed for some ornamental bromeliads in Aechmea in this study. The results showed that the optimal SSR reaction system of Aechmea was a total of 10 μL containing 1×PCR buffer, Mg²⁺ 2.0 mmol·L^-1, dNTPs 200 μmol·L^-1, primers 2.5 μmol·L^-1, template DNA 90 ng and Taq DNA polymerase 1.5 U. Three informative and polymorphic SSR primers, namely M1, M3 and M4, were screened out from 6 SSR primers based on the established SSR reaction system. Three molecular fingerprints were preliminarily constructed for 15 samples of Aechmea species by these three SSR primers, respectively. The establishment of SSR fingerprint proved the SSR reaction system was stable and reliable, and could be further used in germplasm identification of Aechmea.

Key words: Aechmea, SSR, orthogonal experiment design, optimization of reaction system, fingerprint