灵武长枣果实发育过程中阿拉伯半乳糖蛋白组织化学分布

doi:10.7525/j.issn.1673-5102.2022.06.021

摘要/Abstract

摘要：

为揭示灵武长枣（Ziziphus jujuba ‘Lingwu Changzao’）果实发育过程中阿拉伯半乳糖蛋白（AGPs）的分布规律，以膨大前期、快速膨大期、着色期和完熟期灵武长枣果实为试验材料，通过组织化学和免疫荧光定位的方法，研究了不同发育时期果实AGPs的分布特征。结果显示：βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原在各时期果实的外果皮及相邻的内部数层排列紧密的中果皮小细胞的细胞壁和细胞内部均有分布。中果皮大型卵圆形薄壁细胞的细胞壁和细胞内在膨大前期均有βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原分布，而在快速膨大期、着色期和完熟期均主要分布于薄壁细胞的细胞壁上，大部分细胞内部无分布；随着果实发育成熟，中果皮薄壁细胞间隙拉大排列更加松散，出现细胞破裂，βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原分布逐渐减少。各时期果实维管束中维管束鞘、木质部、韧皮部、形成层的所有细胞的细胞壁和细胞内部都分布有βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原，维管束数量和大小随果实发育及体积的进一步增大逐渐减少，βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原分布逐渐减少。研究认为，βGlcY和MAC204分别为研究灵武长枣果实AGPs组化定位有效方法和免疫荧光的良好抗体，各时期果实的不同组织βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原荧光强弱存在一定的差异；AGPs可能参与了灵武长枣果实维管束发育过程的形态建成；为果实发育提供保护和营养支持及多糖物质积累。

关键词: 灵武长枣, 果实, AGPs, 免疫组化定位

Abstract:

To reveal the distribution of Arabinogalactan proteins（AGPs）of Ziziphus jujuba ‘Lingwu Changzao’during fruit development， fruits in the early bulking period， rapid enlargement period， coloring period and maturation period were used as materials and the distribution of AGPs were identified by methods of histochemistry and immunofluorescence localization respectively. The results showed the brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and cell interiors of the epicarp and the alongside internal layers of closely arranged mesocarp small cells in different periods fruit. The brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and the inner part of the large ovoid parenchyma cells in the inner mesocarp during the early bulking period， while they were mainly distributed in the parenchyma cell walls during the rapid enlargement period， coloring period and maturation periods， with no distribution in the inner part of most cells. With the ripening of fruits， the mesocarp parenchyma cells became more loosely arranged with elongated intercellular spaces， cell rupture occurred， and the distribution of brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody gradually decreased. The brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and cell interiors of all cells in the vascular bundle sheaths， xylem， phloem， and cambium of vascular bundles in different periods fruit respectively， and the number and size of vascular bundles gradually decreased with fruit development and further increased in size， and the distribution of brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody gradually decreased. The results suggested that βGlcY and MAC204 were respectively effective method for histochemical localization and good antibody for immunofluorescence of Z. jujuba ‘Lingwu Changzao’ fruit， and there were some differences in brown red precipitate formed by βGlcY and the fluorescence intensity of antigen recognized by MAC204 antibody in different tissues of fruits at different periods， AGPs probably participated in morphogenesis during vascular bundles development of Z. jujuba ‘Lingwu Changzao’ fruit， which provided protection and nutritional support and polysaccharide accumulation for fruit development.

Key words: Ziziphus jujuba ‘Lingwu Changzao’, fruit, AGPs, immunohistochemical localization

中图分类号:

S665.1

王静, 章英才, 陶珊珊. 灵武长枣果实发育过程中阿拉伯半乳糖蛋白组织化学分布[J]. 植物研究, 2022, 42(6): 1106-1120.

Jing WANG, Yingcai ZHANG, Shanshan TAO. Histochemical Localization of Arabinogalactan Proteins During Fruit Development from Ziziphus jujuba ‘Lingwu Changzao’[J]. Bulletin of Botanical Research, 2022, 42(6): 1106-1120.

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参考文献 24

1	MAKAROVA E N， SHAKHMATOV E G， BELYY V A.Structural studies of water-extractable pectic polysaccharides and arabinogalactan proteins from Picea abies greenery［J］.Carbohydrate Polymers，2018，195：207-217.
2	马浩力，余礼，梁荣洪，等.高等植物阿拉伯半乳糖蛋白的功能研究［J］.中国科学：生命科学，2015，45（2）：113-123.
	MA H L， YU L， LIANG R H，et al.Functional studies of arabinogalactan proteins in higher plants［J］.Scientia Sinica Vitae，2015，45（2）：113-123.
3	LESZCZUK A， SZCZUKA E.Arabinogalactan proteins：immunolocalization in the developing ovary of a facultative apomict Fragaria x ananassa（Duch.）［J］.Plant Physiology and Biochemistry，2018，123：24-33.
4	秦源，陈丹，赵洁.烟草柱头和花柱中阿拉伯半乳糖蛋白的定位［J］.植物生理与分子生物学学报，2006，32（3）：307-314.
	QIN Y， CHEN D， ZHAO J.The localization of arabinogalactan-proteins in stigma and style of Nicotiana tabacum L.［J］.Journal of Plant Physiology and Molecular Biology，2006，32（3）：307-314.
5	SHAKHMATOV E G， ATUKMAEV K V， MAKAROVA E N.Structural characteristics of pectic polysaccharides and arabinogalactan proteins from Heracleum sosnowskyi Manden［J］.Carbohydrate Polymers，2016，136：1358-1369.
6	LESZCZUK A， SZCZUKA E， ZDUNEK A.Arabinogalactan proteins：distribution during the development of male and female gametophytes［J］.Plant Physiology and Biochemistry，2019，135：9-18.
7	CANNESAN M A， DURAND C， BUREL C，et al.Effect of arabinogalactan proteins from the root caps of pea and Brassica napus on Aphanomyces euteiches zoospore chemotaxis and germination［J］.Plant Physiology，2012，159（4）：1658-1670.
8	JIA Q S， ZHU J， XU X F，et al. Arabidopsis AT-hook protein TEK positively regulates the expression of arabinogalactan proteins for nexine formation［J］.Molecular Plant，2015，8（2）：251-260.
9	LESZCZUK A， CHYLIŃSKA M， ZIĘBA E，et al.Structural network of arabinogalactan proteins（AGPs） and pectins in apple fruit during ripening and senescence processes［J］.Plant Science，2018，275：36-48.
10	REDGWELL R J， CURTI D， WANG J K，et al.Cell wall polysaccharides of Chinese wolfberry（Lycium barbarum）：Part 2.Characterisation of arabinogalactan-proteins［J］.Carbohydrate Polymers，2011，84（3）：1075-1083.
11	BAO H， ZHENG G Q， QI G L，et al.Cellular localization and levels of arabinogalactan proteins in Lycium barbarum’s fruits［J］.Pakistan Journal of Botany，2016，48（5）：1951-1963.
12	田庚元.枸杞子糖缀合物的结构与生物活性研究［J］.世界科学技术-中医药现代化，2003，5（4）：22-30.
	TIAN G Y.Study on structure and bioactivity of glycoconjugate compounds of fructus lycii［J］.World Science and Technology-Modernization of Traditional Chinese Medicine and Materia Medica，2003，5（4）：22-30.
13	章英才，柴雅红，曹金霞.灵武长枣果实多糖中单糖组成分析［J］.干旱地区农业研究，2018，36（2）：144-152.
	ZHANG Y C， CHAI Y H， CAO J X.Monosaccharide composition of polysaccharides in Ziziphus jujuba Mill. cv.Lingwu Changzao fruit［J］.Agricultural Research in the Arid Areas，2018，36（2）：144-152.
14	TRIFUNOVIĆ M， TADIĆ V， PETRIĆ M，et al.Quantification of arabinogalactan proteins during in vitro morphogenesis induced by β-D-glucosyl Yariv reagent in Centaurium erythraea root culture［J］.Acta Physiologiae Plantarum，2014，36（5）：1187-1195.
15	CHAPMAN A， BLERVACQ AS， VASSEUR J，et al.Arabinogalactan-proteins in Cichorium somatic embryogenesis：effect of β-glucosyl Yariv reagent and epitope localisation during embryo development［J］.Planta，2000，211（3）：305-314.
16	LESZCZUK A， SZCZUKA E， LEWTAK K，et al.Effect of low temperature on changes in AGP distribution during development of Bellis perennis ovules and anthers［J］.Cells，2021，10（8）：1880-1893.
17	LESZCZUK A， SZCZUKA E， WYDRYCH J，et al.Changes in arabinogalactan proteins（AGPs） distribution in apple（Malus x domestica） fruit during senescence［J］.Postharvest Biology and Technology，2018，138：99-106.
18	任玉锋，姜牧炎，马文平，等.灵武长枣采后低温贮藏期间细胞壁组分的变化［J］.北方园艺，2018（18）：114-117.
	REN Y F， JIANG M Y， MA W P，et al.Changes of cell wall components in fruit of Zizphus jujube Mill.cv.Lingwu Changzao at low temperature during the postharvest period［J］.Northern Horticulture，2018（18）：114-117.
19	XU C X， TAKÁČ T， BURBACH C，et al.Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana（Musa spp.AAA）［J］.BMC Plant Biology，2011，11（1）：38.
20	ELLIS M， EGELUND J， SCHULTZ C J，et al.Arabinogalactan-proteins：key regulators at the cell surface？［J］.Plant Physiology，2010，153：403-419.
21	YANG J， SARDAR H S， MCGOVERN K R，et al.A lysine-rich arabinogalactan protein in Arabidopsis is essential for plant growth and development，including cell division and expansion［J］.The Plant Journal，2007，49（4）：629-640.
22	MOTOSE H， SUGIYAMA M， FUKUDA H.A proteoglycan mediates inductive interaction during plant vascular development［J］.Nature，2004，429：873-878.
23	LIU C G， MEHDY M C.A nonclassical arabinogalactan protein gene highly expressed in vascular tissues，AGP31，is transcriptionally repressed by methyl jasmonic acid in Arabidopsis ［J］.Plant Physiology，2007，145：863-874.
24	黄月，章英才，苏伟东，等.灵武长枣果实维管束韧皮部及周围细胞的超微结构特征［J］.广西植物，2021，41（1）：133-143.
	HUANG Y， ZHANG Y C， SU W D，et al.Ultrastructural characteristics of Ziziphus jujuba cv.Lingwuchangzao fruit vascular bundles phloem and its surrounding parenchyma cells［J］.Guihaia，2021，41（1）：133-143.

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