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植物研究 ›› 2022, Vol. 42 ›› Issue (3): 446-454.doi: 10.7525/j.issn.1673-5102.2022.03.014

• 分子生物学 • 上一篇    下一篇

苦马豆叶绿体基因组结构及其特征分析

苏丹丹1,2, 刘玉萍1,2, 刘涛1,2, 郑长远1,2, 张雨1,2, 王亚男1,2, 秦娜1,2, 苏旭1,2,3()   

  1. 1.青海师范大学生命科学学院,西宁 810008
    2.青海师范大学青海省青藏高原药用动植物资源重点实验室,西宁 810008
    3.高原科学与可持续发展研究院,西宁 810016
  • 收稿日期:2021-04-28 出版日期:2022-05-20 发布日期:2022-05-20
  • 通讯作者: 苏旭 E-mail:xusu8527972@126.com
  • 作者简介:苏丹丹(1998—),女,硕士研究生,主要从事高山植物系统与进化研究。
  • 基金资助:
    科技基础资源调查专项项目(2017FY100202-02);祁连山国家公园青海研究中心开放课题;青海省青藏高原药用动植物资源重点实验室项目(2020-ZJ-Y40)

Structure of Chloroplast Genome and its Characteristics of Sphaerophysa salsula

Dandan SU1,2, Yuping LIU1,2, Tao LIU1,2, Changyuan ZHENG1,2, Yu ZHANG1,2, Yanan WANG1,2, Na QIN1,2, Xu SU1,2,3()   

  1. 1.School of Life Science,Qinghai Normal University,Xining 810008
    2.Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province,Qinghai Normal University,Xining 810008
    3.Academy of Plateau Science and Sustainability,Qinghai Normal University,Xining 810016
  • Received:2021-04-28 Online:2022-05-20 Published:2022-05-20
  • Contact: Xu SU E-mail:xusu8527972@126.com
  • About author:SU Dandan(1998—),female,postgraduate,mainly engaged in the system and evolution of alpine plants.
  • Supported by:
    Special Project for Scientific-Technological Basic Resource Investigation(2017FY100202-02);Open Project from Qinghai Research Center of Qilian Mountain National Park;Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibet Plateau in Qinghai Province(2020-ZJ-Y40)

摘要:

利用Illumina高通量测序平台对苦马豆(Sphaerophysa salsula)进行测序和组装,获得完整的苦马豆叶绿体基因组序列。组装结果表明,苦马豆叶绿体基因组全长123 327 bp,存在IR区丢失,不具有四分体结构;注释结果显示,苦马豆叶绿体基因组共编码108个基因,其中包含74个蛋白编码基因、4个rRNA基因和30个tRNA基因;叶绿体基因组序列上共检测到99个SSR位点,包含75个单核苷酸、17个二核苷酸和7个三核苷酸重复单元;系统发育分析显示,苦马豆和骆驼刺为姊妹群,亲缘关系最近。这为今后苦马豆遗传多样性、群体遗传结构和物种形成机制研究奠定了基础。

关键词: 苦马豆, 叶绿体基因组, 简单重复序列, 密码子偏好性, 系统发育

Abstract:

The whole chloroplast genome of Sphaerophysa salsula was sequenced and assembled based on Illumina high-throughput sequencing platform. The assembly result indicated that the whole chloroplast genome of S. salsula had a total length of 123 327 bp, with a loss of IR region. The chloroplast genome encoded a total of 108 genes, including 74 protein-coding genes, 4 rRNA genes and 30 tRNA genes. and 99 SSR loci were found, and among them the number of mononucleotide, dinucleotide and trinucleotide repeat motifs was 75, 17 and 7, respectively. The phylogenetic analysis results showed that S. salsula and Alhagi sparsifolia were sister species, which had the closest genetic relationship. The results would lay the foundation for the genetic diversity, population genetic structure and speciation mechanism of S. salsula in the future.

Key words: Sphaerophysa salsula, chloroplast genome, simple sequence repeats, codon preference, phylogeny

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