萱草叶片响应低温胁迫的转录组分析

doi:10.7525/j.issn.1673-5102.2022.03.012

摘要/Abstract

摘要：

低温胁迫是萱草（Hemerocallis fulva）生长过程中经常会遭遇的一种非生物胁迫。比较了萱草叶片在低温处理（10、5、0 ℃）下转录组与对照（15 ℃）数据的差异，共筛选出差异表达基因2 457个，其中上调基因1 253个，下调基因1 204个。差异表达基因主要富集在细胞过程、代谢过程和催化活性等49个GO过程，代谢途径、次生代谢产物的生物合成、植物激素信号转导等42条KEGG代谢途径中。其中参与植物激素信号转导通路的差异表达基因发生了不同程度的变化，GH3.10基因上调至对照组的13.624倍，IAA1基因下调0.120倍；参与可溶性糖合成通路的差异基因发生了0.076~28.114倍不同程度的变化。随后对3个低温处理组共有的29个差异表达基因进行热图和网络调控分析，基于基因在网络调控中的位置，对ABCF5、OFPs和SWEETs等基因在冷应答的作用进行了分析。本研究结果为进一步挖掘萱草低温响应的关键基因及耐寒萱草种质开发、分子育种提供了一定的理论支撑。

关键词: 萱草, 低温胁迫, 转录组, 激素, 可溶性糖

Abstract:

Low temperature is a kind of abiotic stress in the growth process of Hemerocallis fulva. The transcriptome data of Hemerocallis fulva leaves under cold treatment groups （10， 5， 0 ℃） and control group （15℃） was compared， and a total of 2 457 DEGs were screened， including 1 253 up-regulated genes and 1 204 down-regulated genes. The results showed that differentially expressed genes（DEGs） were mainly concentrated in 49 GO processes such as cellular process， metabolic process and catalytic activity， and 42 KEGG metabolic pathways， including metabolic pathways， biosynthesis of secondary metabolites and plant hormone signal transduction. Among them， the DEGs involved in plant hormone signal transduction pathway changed in varying degrees， GH3.10 gene was increased to 13.624 times higher than the control group， IAA1 gene was down-regulated to 0.120 times； the DEGs involved in soluble sugar synthesis pathway also changed by 0.076-28.114 times. Then， 29 DEGs shared by treatment groups were analyzed by heat map and gene regulation networks. Based on the position of genes in gene regulation networks， the roles of ABCF5， OFPs and SWEETs in response to cold were analyzed respectively. The results of this study provide a theoretical basis for further exploring the key genes of low temperature response， germplasm development and molecular breeding of Hemerocallis fulva.

Key words: Hemerocallis fulva, low temperature stress, transcriptome, hormone, soluble sugar

中图分类号:

S682

黄东梅, 陈颖, 白露, 倪迪安, 徐奕扬, 张志国, 秦巧平. 萱草叶片响应低温胁迫的转录组分析[J]. 植物研究, 2022, 42(3): 424-436.

Dongmei HUANG, Ying CHEN, Lu BAI, Di’an NI, Yiyang XU, Zhiguo ZHANG, Qiaoping QIN. Transcriptome Analysis of Hemerocallis fulva Leaves Respond to Low Temperature Stress[J]. Bulletin of Botanical Research, 2022, 42(3): 424-436.

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基因 Gene ID	正向序列 Sense primes（5′—3′）	反向序列 Antisense primers（3′—5′）
内参UBQ	AACGTGAAGGCCAAGATAC	AGACGGAGCACCAGGTGGA
DN3322_c0_g1	TATCTCGTCCATCCTGTC	TCCATCGGTCTTTCTATT
DN56313_c2_g3	TAGCGATGAGGATAGAATA	CCCGGCTTTGGACTGGAA
DN61425_c1_g1	AACTGCGGCTCGGTTGAG	CCCACGGCTTTGATTTCG
DN60592_c1_g2	TTGTATCGGTTTAGGTGA	AACATTGGTTTACGCTTG
DN56941_c1_g4	TGAGGAGCAAAACCAAAA	CCTCCCCGAAGCCACAAC
DN62545_c4_g4	AAATACGTGGCAAATCGT	CAACACCCAAGAGCAAAA
DN60404_c3_g2	GGGGAATGTGATATGACTG	CGCAACTTGTAGGGAGAT
DN48576_c0_g1	GGAGGAAGCACAAGCAGC	CAGCAAGCAAGTCGGATG
DN59631_c1_g1	GGCAGAAACTGAAACTAA	CATTCGGACCATACAAGG
DN60001_c3_g1	CTGCATAAAGGCCATAGT	CTTCACCCAATACCAATC
DN487206_c0_g1	CTCACAACGGTTCATCCA	AAGAGGGAGTAGGGAATG
DN61874_c2_g2	GCTGACATCTCGTGGTTC	CAGCCTTGCCACAAATAA

Group	Raw_reads	Clean_reads	Clean_bases	Error /%	Q20 /%	Q30 /%	GC /%
T₀	46 929 604	46 376 031	6.44GB	0.02	98.24	94.57	45.47
T₁	42 571 478	42，120 296	5.86GB	0.02	98.18	94.45	45.17
T₂	41 846 630	41 392 792	5.74GB	0.02	98.29	94.68	45.35
T₃	44 823 304	44 378 448	6.16GB	0.02	98.30	94.73	45.88

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