阴阳离子表面活性剂对无花果叶中有效成分提取工艺优化及初步功能评价

doi:10.7525/j.issn.1673-5102.2020.05.019

植物研究 ›› 2020, Vol. 40 ›› Issue (5): 789-794.doi: 10.7525/j.issn.1673-5102.2020.05.019

阴阳离子表面活性剂对无花果叶中有效成分提取工艺优化及初步功能评价

孟瑶^1,2,3, 余莲^1,2,3, 秦祥宇^1,2,3, 刘畅^1,2,3, 王泽霖^1,2,3, 赵春建^1,2,3, 付玉杰^1,2,3

1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
2. 东北林业大学林业生物制剂教育部工程研究中心, 哈尔滨 150040;
3. 东北林业大学化学化工与资源利用学院, 哈尔滨 150040

收稿日期:2020-04-07 出版日期:2020-09-05 发布日期:2020-07-10
通讯作者: 付玉杰,E-mail:yujie-fu@163.com E-mail:yujie-fu@163.com
作者简介:孟瑶(1996-),女,硕士研究生,主要从事天然产物的提取分离研究。
基金资助:
“十三五”国家重点研发计划项目（2016YFD0600805）；黑龙江省应用技术研究与开发项目（GX18B003）；111创新引智项目（B20088）

Optimization of the ActiveCompoundsExtraction Process from Fig Leaves by Anionic and Cationic Surfactants and Preliminary Functional Evaluation

MENG Yao^1,2,3, YU Lian^1,2,3, QIN Xiang-Yu^1,2,3, LIU Chang^1,2,3, WANG Ze-Lin^1,2,3, ZHAO Chun-Jian^1,2,3, FU Yu-Jie^1,2,3

1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
2. Engineering Research Center of Forestry Biological Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040;
3. Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040

Received:2020-04-07 Online:2020-09-05 Published:2020-07-10
Supported by:
National Key R&D Program of China(2016YFD0600805);Heilongjiang Province Applied Technology Research and Development Project(GX18B003);Innovative and intelligent Projects 111(B20088)

摘要/Abstract

摘要： 对阴阳离子复配型表面活性剂联合微波辅助提取无花果叶中有效活性物质的实验条件进行了设计和工艺参数的优化，最终的结果如下：1%（w/v）阴阳离子复配型表面活性剂，提取温度40℃，提取时间10 min，液固比30：1 mL·g^-1。在上述优化的条件下补骨脂素及佛手柑内酯的平均提取率分别为15.37和3.59 mg·g^-1。通过将利用表面活性剂辅助提取与常规水溶液提取获得的无花果叶粗提物进行对比发现，加入了表面活性剂的溶液抗氧化能力有明显增强，同时无花果叶主要物质的降解也得到显著的抑制，对植物中天然有效目标产物的绿色高效提取以及功能评价具有十分重要的理论指导意义。

关键词: 无花果叶, 表面活性剂, 微波辅助提取, 单因素实验

Abstract: The experimental conditions for formulated surfactant combined with microwave-assisted extraction of effective bioactive substances in fig leaves were designed and the process parameters were optimized. The final results were as follows:1%(w/v) formulated surfactant(sodium dodecyl sulfate(SDS):dodecyl trimethyl ammonium bromide(DTAB)), extraction temperature of 40℃, extraction time of 10 min, liquid-solid ratio of 30:1 mL·g^-1. Under the above optimized conditions, the average extraction yields of psoralen and bergapten were 15.37 and 3.59 mg·g^-1, respectively. The antioxidant activity of crude fig extract obtained by surfactant-assisted extraction was significantly enhanced as compared with that by conventional aqueous extraction. Meanwhile, the degradation rate of the main substance in fig leaveswas also significantly inhibited. This study provides the necessary scientific basis for the rational and effective development and utilization of fig leaf resources. Also, it is of great theoretical significance for the green and efficient extraction of natural and effective target products and functional evaluation in plants.

Key words: fig leaf, surfactant, microwave-assisted extraction, single factor experiment

中图分类号:

S663.3

孟瑶, 余莲, 秦祥宇, 刘畅, 王泽霖, 赵春建, 付玉杰. 阴阳离子表面活性剂对无花果叶中有效成分提取工艺优化及初步功能评价[J]. 植物研究, 2020, 40(5): 789-794.

MENG Yao, YU Lian, QIN Xiang-Yu, LIU Chang, WANG Ze-Lin, ZHAO Chun-Jian, FU Yu-Jie. Optimization of the ActiveCompoundsExtraction Process from Fig Leaves by Anionic and Cationic Surfactants and Preliminary Functional Evaluation[J]. Bulletin of Botanical Research, 2020, 40(5): 789-794.

参考文献

[1] Cheng J,Yi X M,Wang Y H,et al.Phenolics from the roots of hairy fig(Ficus hirta Vahl.) exert prominent anti-inflammatory activity[J].Journal of Functional Foods,2017,31:79-88.
[2] Conforti F,Menichini G,Zanfini L,et al.Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma[J].Cell Proliferation,2012,45(3):279-285.
[3] Liu F F,Yang Z S,Zheng X,et al.Nematicidal coumarin from Ficus carica L.[J].Journal of Asia-Pacific Entomology,2011,14(1):79-81.
[4] Pérezc,Domíngueze,Canal J R,et al.Hypoglycaemic activity of an aqueous extract from Ficus carica(fig tree) leaves in streptozotocin diabetic rats[J].Pharmaceutical Biology,2000,38(3):181-186.
[5] Canal J R,Torres M D,Romero A,et al.A chloroform extract obtained from a decoction of Ficus carica leaves improves the cholesterolaemic status of rats with streptootocin-includede diabetes[J].Acta Physiologica Hungarica,2000,87(1):71-76.
[6] Kitajima S,Aoki W,Shibata D,et al.Comparative multi-omics analysis reveals diverse latex-based defense strategies against pests among latex-producing organs of the fig tree(Ficus carica)[J].Planta,2018,247(6):1423-1438.
[7] Qin L Z,Chen H Z.Enhancement of flavonoids extraction from fig leaf using steam explosion[J].Industrial Crops and Products,2015,69:1-6.
[8] 沙坤,张泽俊.微波辅助提取无花果叶中补骨脂素工艺的研究[J].食品科技,2010,35(8):244-246.Sha K,Zhang Z J.Study on extraction of psoralen from Ficus carica leaves by microwave-assisted method[J].Food Science and Technology,2010,35(8):244-246.
[9] Takahashi T,Okiura A,Kohno M.Phenylpropanoid composition in fig(Ficus carica L.) leaves[J].Journal of Natural Medicines,2017,71(4):770-775.
[10] Veberic R,Colari M,Stampar F.Phenolic acids and flavonoids of fig fruit(Ficus carica L.) in the northern Mediterranean region[J].Food Chemistry,2008,106(1):153-157.
[11] 崔鹏,陈志冉,史雷革.微波提取无花果叶中的总黄酮及其体外抗氧化活性研究[J].食品研究与开发,2018,39(1):41-45.Cui P,Chen Z R,Shi L G.Microwave extraction and free radical scavenging activity of total flavonoids from Ficus carica leaves[J].Food Research and Development,2018,39(1):41-45.
[12] Khoddami A,Wilkes M A,Roberts T H.Techniques for analysis of plant phenolic compounds[J].Molecules,2013,18(2):2328-2375.
[13] Sharma S,Kori S,Parmar A.Surfactant mediated extraction of total phenolic contents(TPC) and antioxidants from fruits juices[J].Food Chemistry,2015,185:284-288.
[14] 李富兰,颜杰.生物表面活性剂的特性及其在环境污染治理中的应用[J].安徽农业科学,2011,39(11):6651-6652.Li F L,Yan J.Advances in application of biosurfactants on environmental pollution control[J].Journal of Anhui Agricultural Sciences,2011,39(11):6651-6652.
[15] 尹凯,郭晓锋,陶彭均,等.生物表面活性剂的合成及应用研究[J].精细与专用化学品,2011,19(2):43-46.Yin K,Guo X F,Tao P J,et al.Study on synthesis and application of the biosurfactant[J].Fine and Specialty Chemicals,2011,19(2):43-46.

阴阳离子表面活性剂对无花果叶中有效成分提取工艺优化及初步功能评价

Optimization of the ActiveCompoundsExtraction Process from Fig Leaves by Anionic and Cationic Surfactants and Preliminary Functional Evaluation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	杨建航, 赵修华. 胶束介导联合超声—微波辅助法提取喜树叶中喜树碱及羟基喜树碱[J]. 植物研究, 2020, 40(6): 951-960.
[2]	朱亚伟, 王立涛, 安娟艳, 吕慕洁, 孙建航, 郭娜, 付玉杰. 响应面优化酶解—微波辅助提取桑叶多糖的工艺研究[J]. 植物研究, 2020, 40(4): 635-640.
[3]	祖述冲. 松籽油的干式酶法提取工艺优化与理化性质分析[J]. 植物研究, 2020, 40(2): 308-313.
[4]	李占君, 刘运伟, 张忠林, 张志环, 马珂, 杨逢建. 塔拉多糖的脱色工艺化研究[J]. 植物研究, 2018, 38(3): 475-480.
[5]	杨明非, 苏雯, 寇萍, 王希清, 郭娜, 臧玉萍, 付玉杰. 高速匀质-微波辅助提取红松籽油工艺及其品质评价[J]. 植物研究, 2017, 37(5): 789-796.
[6]	王化1,2；周丽萍1,2；李梦莎1；朱良玉1,2；穆立蔷2；倪红伟1*. 微波辅助提取暴马丁香中紫丁香苷和橄榄苦苷的工艺研究[J]. 植物研究, 2016, 36(1): 141-145.
[7]	马飞跃;段开放;王希清;秦明聪;李吉;付玉杰*. 离子液体微波辅助提取降香檀叶中鹰嘴豆芽素A和染料木素[J]. 植物研究, 2013, 33(4): 494-498.
[8]	彭霄;段明慧;潘有智;张东阳;段喜华;*. 微波辅助提取木豆根中染料木素工艺[J]. 植物研究, 2012, 32(6): 756-759.