皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究

doi:10.7525/j.issn.1673-5102.2017.06.018

植物研究 ›› 2017, Vol. 37 ›› Issue (6): 941-946.doi: 10.7525/j.issn.1673-5102.2017.06.018

皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究

杨明非¹, 张晶³, 苏雯², 王鹏³, 张孙东³, 寇萍³, 焦骄³, 杨雨春⁴, 付玉杰³

1. 北日本制药株式会社产品研发室, 日本富山县中新川郡上市町若杉55号 930-0314;
2. 广东省珠海市斗门区公立医疗卫生机构管理中心, 珠海 519125;
3. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
4. 吉林省林业科学院, 长春 130033

收稿日期:2017-03-15 出版日期:2017-11-15 发布日期:2017-11-25
通讯作者: 付玉杰 E-mail:yujie_fu@163.com
作者简介:杨明非(1989-),男,临床医学硕士,主要从事肿瘤药物研究。
基金资助:
“十三五”国家重点研发计划项目（2016YFD0600805）

The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

YANG Ming-Fei¹, ZHANG Jing³, SU Wen², WANG Peng³, ZHANG Sun-Dong³, KOU Ping³, JIAO Jiao³, YANG Yu-Chun⁴, FU Yu-Jie³

1. Research and Development Department of Kitanihon Pharmaceutical CO., No. 55, Kamiichi, Nakaniikawa District, Toyama, Japan 930-0314;
2. Management Center of Public Medical and Health Institutions in Doumen District, Zhuhai City, Guangdong Province, Zhuhai 519125;
3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
4. Forestry Academy of Jilin Province, Changchun 130033

Received:2017-03-15 Online:2017-11-15 Published:2017-11-25
Supported by:
"13th Five-Year" The National Key Research Projects(2016YFD0600805)

摘要/Abstract

摘要： 针对单一活性成分的减肥降脂效果较差，且容易出现耐药性等问题，本文筛选并优化了皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比，为皮诺敛酸降脂产品的开发提供基础数据。通过油酸诱导HepG2细胞成脂建立体外非酒精性脂肪肝模型。采用油红O染色法确定了皮诺敛酸/左旋肉碱降低HepG2细胞中脂滴的最佳浓度配比。实验结果表明0.5 mmol·L^-1油酸诱导HepG2细胞脂肪变性效果最佳，皮诺敛酸（PLA）最佳降脂浓度为6.25 μmol·L^-1，左旋肉碱（LC）最佳降脂浓度为250 μmol·L^-1，皮诺敛酸/左旋肉碱（PLA/LC）最佳降脂浓度配比为1：40（μmol·L^-1/μmol·L^-1）。皮诺敛酸/左旋肉碱复配物可以显著降低HepG2细胞脂质，具有用量少，协同作用效果突出的优点，因此皮诺敛酸/左旋肉碱复配物在开发降脂减肥产品中具有良好的应用前景。

关键词: 皮诺敛酸, 左旋肉碱, 复配, 降脂, 非酒精性脂肪肝

Abstract: As the single active ingredient is poor and prone to drug resistance on effects of lose weight and lipid-lowering, so the study focuses on screening and optimizing the optimal concentration ratio of pinoleic acid/L-carnitine to reduce the lipid of HepG2 cell, which provides the basic data for the development of pinoleic acid lipid-lowering products. Oleic acid induced HepG2 cell adipogenesis, which was used to simulate the model of nonalcoholic fatty liver in vitro. The optimal concentration in combination of pinoleic acid/L-Carnitine decrease lipid was determinated by oil red O staining. The results show that the optimum concentration of oleic acid-induced HepG2 cell steatosis is 0.5 mmol·L^-1.The optimal lipolysis concentration of pinoleic acid is 6.25 μmol·L^-1. The optimal lipid metabolism concentration of L-Carnitine is 250 μmol·L^-1. The best lipid-lowering ratio of pinoleic acid/L-carnitine(PLA/LC) is 1:40(μmol·L^-1/μmol·L^-1). The characteristics of pinoleic acid and L-Carnitine complexes decreased the doses and improved synergistic effect, Therefore, Combination of pinoleic acid/L-Carnitine has a potential prospectin in the development of lipid-lowering diet products.

Key words: pinoleic acid, L-Carnitine, complexes, lipid-lowering, nonalcoholic fatty liver disease

中图分类号:

Q547

杨明非, 张晶, 苏雯, 王鹏, 张孙东, 寇萍, 焦骄, 杨雨春, 付玉杰. 皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究[J]. 植物研究, 2017, 37(6): 941-946.

YANG Ming-Fei, ZHANG Jing, SU Wen, WANG Peng, ZHANG Sun-Dong, KOU Ping, JIAO Jiao, YANG Yu-Chun, FU Yu-Jie. The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2[J]. Bulletin of Botanical Research, 2017, 37(6): 941-946.

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皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究

The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

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