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植物研究 ›› 2011, Vol. 31 ›› Issue (3): 306-312.doi: 10.7525/j.issn.1673-5102.2011.03.010

• 论文 • 上一篇    下一篇

‘范妮’亚麻高频率体细胞胚胎发生及组织学观察

郭玲玲;苗书魁;计巧灵*;姜丽   

  1. 新疆大学生命科学与技术学院,乌鲁木齐 830046
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 通讯作者: 计巧灵
  • 基金资助:
     

High Frequency Somatic Embryogenesis and Histological Observation on Linum usitatissimum L. ‘Fany’

GUO Ling-Ling;MIAO Shu-Kui;JI Qiao-Ling*;JIANG Li   

  1. College of Life Science and Technology,Xinjiang University,Urumqi 830046
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Contact: JI Qiao-Ling
  • Supported by:
     

摘要: 以亚麻品种‘范妮’的子叶和下胚轴为外植体,分别接种在不同组合的植物生长调节剂的MB(MS培养基的无机盐加B5培养基的维生素)培养基上培养,结果所有使用的培养基都能诱导出愈伤组织,但只有在附加了0.5~4.0 mg·L-1 2,4-D培养基上才能诱导出胚性愈伤组织。在胚性愈伤组织和体胚诱导中,源于下胚轴的材料均比源于子叶的材料所需的2,4-D浓度低。在培养基中,单独使用2,4-D诱导体胚的效果明显好于使用其它植物生长调节剂的组合,源于子叶和下胚轴的体胚诱导数分别达到了224个/g和265个/g。组织学观察发现有大量正常的球形胚、心形胚、鱼雷胚和少量子叶胚,还有少量畸形胚。本文为‘范妮’与其它麻类的体细胞杂交育种、‘范妮’变异无性系的筛选及相关的植物基因工程和细胞工程研究奠定了一定的工作基础。

关键词: &lsquo, 范妮&rsquo, 亚麻, 胚性愈伤组织, 胚状体, 组织学

Abstract: The cotyledon and hypocotyl of Linum usitatissimum L. ‘Fany’ as explants were respectively inoculated on MB medium (mineral salt of MS with vitamin of B5) with different combinations of plant growth regulators. Callus was induced from all the media. However, embryogenic callus were only obtained on the media with 0.5-4.0 mg·L-1 2,4-D. The 2,4-D concentration needed for embryogenic callus and embryogenesis derived from hypocotyl were both lower than that from cotyledon. The effect of supplementing with 2,4-D only in medium was obviously better than the combinations of any other plant growth regulators in the induction of embryos. The induction number of somatic embryos derived from cotyledon and hypocotyl was 224/g and 265/g, respectively. The histology observation revealed that there were a large number of normal globular embryos, heart-stage embryos, torpedo-stage embryos, a few cotyledon-stage embryos as well as a few abnormal embryos. These results will be a foundation for somatic hybridization between ‘Fany’ and other flax, screening mutant clone of ‘Fany’ as well as relative research on plant gene and cell engineering.

Key words: Linum usitatissimum L. &lsquo, Fany&rsquo, embryogenic callus, embryos, histology

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