CO2浓度升高对苦草(Vallisneria natans)叶绿素荧光特性的影响

doi:10.7525/j.issn.1673-5102.2017.01.007

植物研究 ›› 2017, Vol. 37 ›› Issue (1): 45-51.doi: 10.7525/j.issn.1673-5102.2017.01.007

CO₂浓度升高对苦草(Vallisneria natans)叶绿素荧光特性的影响

韩燕青^1,2, 刘鑫², 胡维平², 张平究¹, 邓建才², 成泽霖³

1. 安徽师范大学国土资源与旅游学院, 芜湖 241000;
2. 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;
3. 上海海洋大学水产与生命学院, 上海 201306

收稿日期:2016-09-29 出版日期:2017-01-15 发布日期:2017-03-06
通讯作者: 邓建才,E-mail:jcdeng@niglas.ac.cn E-mail:jcdeng@niglas.ac.cn
作者简介:韩燕青(1991-),男,硕士研究生,主要从事水生植物生态学方面研究。
基金资助:
国家自然科学基金（41271213，41230853）；国家水体污染控制与治理科技重大专项（2014ZX07101-011）；中国科学院重点部署项目（KZZD-EW-10）

Effects of CO₂ Enrichment on Chlorophyll Fluorescence Characteristics of Vallisneria natans

HAN Yan-Qing^1,2, LIU Xin², HU Wei-Ping², ZHANG Ping-Jiu¹, DENG Jian-Cai², CHENG Ze-Lin³

1. College of Territorial Resources and Tourism, Anhui Normal University, Wuhu 241000;
2. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008;
3. College of Fisheries and life science, Shanghai Ocean University, Shanghai 201306

Received:2016-09-29 Online:2017-01-15 Published:2017-03-06
Supported by:
This research is supported by National Natural Science Foundation(41271213, 41230853);State Water Pollution Control and Management Technology Major Projects(2014ZX07101-011);Chinese Academy of Sciences Key Deployment Project(KZZD-EW-10)

摘要/Abstract

摘要： 为了探究大气CO₂升高对沉水植物光合生理的影响，利用便携式植物效率分析仪（Handy PEA），在无损的情况下测定不同CO₂浓度处理下的苦草（Vallisneria natans）叶绿素荧光诱导曲线，并采用JIP-test分析方法分析数据，研究CO₂浓度对苦草叶片叶绿素荧光特性的影响。结果表明在实验进行60 d后，与对照相比，高CO₂浓度处理下的苦草叶片PSⅡ反应中心受体侧荧光参数V_j、M_o显著升高，S_m、ψ_o、φ_Eo显著降低，叶片电子传递能力减弱；K相相对可变荧光W_k显著提高，PSⅡ反应中心供体侧放氧复合体OEC受到伤害；ABS/RC、DI_o/RC、TR_o/RC、DI_o/CS_o显著升高，ET_o/RC、RE_o/RC、ET_o/CS_o、RE_o/CS_o显著降低，苦草叶片用于热耗散的能量显著增加，导致用于电子传递及传递到电子链末端的能量显著减少；性能参数F_v/F_m、PI_abs显著降低，苦草叶片PSⅡ潜在活性和光合作用原初反应过程受到抑制。以上结果表明，在长期高CO₂浓度处理下，苦草叶片光合机构功能受到抑制，PSⅡ反应中心活性降低，光合功能下调，发生光适应现象。

关键词: 苦草, CO₂浓度升高, 光系统Ⅱ, 叶绿素荧光

Abstract: To explore the effects of elevated atmospheric CO₂ on photosynthetic physiology of submerged plant, we studied the effects of high concentrations of CO₂ on chlorophyll fluorescence characteristics of Vallisneria natans by measuring the rapid fluorescence induction kinetics curves(OJIP) of the leaves with Plant Efficiency Analyzer and analyzing with JIP-test. The V_j, M_o of V.natans were significantly increased and S_m, ψ_o and φ_Eo were significantly reduced after 60 days, and the electron transfer diminished capacity of V.natans was decreased compared with the control; the donor side of PSⅡ reaction center was hurt because the Wk was increased; the specific activity parameters ABS/RC, DI_o/RC, TR_o/RC, DI_o/CS_o were significantly increased, ET_o/RC, RE_o/RC, ET_o/CS_o, RE_o/CS_o significantly decreased, and the energy for heat dissipation significant increased lead to the energy of electron transfer and electron transfer chain end were significantly decreased. Compared with the control, the performance parameters F_v/F_m and PI_abs were also decreased. Therefore, the long-term high concentrations of CO₂ suppressed photosynthetic apparatus state, decreased PSⅡreaction center activity and down regulated photosynthetic function of V.natans, and the V.natans appeared light acclimation.

Key words: Vallisneria natans, CO₂ enrichment, PSⅡ, chlorophyll fluorescence

中图分类号:

Q945.11

韩燕青, 刘鑫, 胡维平, 张平究, 邓建才, 成泽霖. CO₂浓度升高对苦草(Vallisneria natans)叶绿素荧光特性的影响[J]. 植物研究, 2017, 37(1): 45-51.

HAN Yan-Qing, LIU Xin, HU Wei-Ping, ZHANG Ping-Jiu, DENG Jian-Cai, CHENG Ze-Lin. Effects of CO₂ Enrichment on Chlorophyll Fluorescence Characteristics of Vallisneria natans[J]. Bulletin of Botanical Research, 2017, 37(1): 45-51.

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CO₂浓度升高对苦草(Vallisneria natans)叶绿素荧光特性的影响

Effects of CO₂ Enrichment on Chlorophyll Fluorescence Characteristics of Vallisneria natans

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