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植物研究 ›› 2001, Vol. 21 ›› Issue (1): 113-115.

• 论文 • 上一篇    下一篇

高效液相色谱法测定红景天甙含量方法的研究

王洋1, 张璞2, 于涛1, 王化田1, 阎秀峰1   

  1. 1. 东北林业大学森林植物生态学开放研究实验室, 哈尔滨 150040;
    2. 沈阳药科大学基础部有机教研室, 沈阳 110015
  • 收稿日期:2000-05-18 出版日期:2001-03-15 发布日期:2016-06-14
  • 作者简介:王洋(1971-), 女, 博士, 从事植物化学研究。
  • 基金资助:
    国家自然科学基金资助项目(No.39700013)

STUDY ON THE METHOD FOR DETERMINATION OF SALIDROSIDE BY HPLC

WANG Yang1, ZHANG Pu2, YU Tao1, WANG Hua-tian1, YAN Xiu-feng1   

  1. 1. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040;
    2. Division of Organic Chemistry, Basic Department, Shenyang Pharmaceutical U
  • Received:2000-05-18 Online:2001-03-15 Published:2016-06-14

摘要: 以高效液相色谱法作为红景天甙含量检测方法, 系统探讨了用超声法从高山红景天根中提取红景天甙用以制备分析样品的方法。确定的最佳提取条件为:以蒸馏水为提取溶剂在60℃下超声提取40 min。

关键词: 高山红景天, 红景天甙, 高效液相色谱法, 超声提取法

Abstract: The determination of salidroside in roots of Rhodiola sachalinensis by HPLC were studied. The extracted conditions of salidroside using ultrasonic extraction method from Rhodiola sachalinensis to prepare assay sample were systematacially discussed. The optimal conditions were distilled water as the solvent, 60℃ as the extracted temperature and 30 min as the extracted time.

Key words: Rhodiola sachalinensis, Salidroside, HPLC, Ultrasonic extraction method